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Protein

Putative RNA polymerase II subunit B1 CTD phosphatase Rpap2

Gene

Rpap2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100ZincPROSITE-ProRule annotation1
Metal bindingi105ZincPROSITE-ProRule annotation1
Metal bindingi136ZincPROSITE-ProRule annotation1
Metal bindingi140ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri77 – 160RTR1-typePROSITE-ProRule annotationAdd BLAST84

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative RNA polymerase II subunit B1 CTD phosphatase Rpap2 (EC:3.1.3.16)
Alternative name(s):
RNA polymerase II-associated protein 2
Gene namesi
Name:Rpap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2141142. Rpap2.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Shuttles between the cytoplasm and the nucleus in a CRM1-dependent manner.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002506492 – 614Putative RNA polymerase II subunit B1 CTD phosphatase Rpap2Add BLAST613

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei9PhosphoserineBy similarity1
Modified residuei217PhosphoserineBy similarity1
Modified residuei481PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ8VC34.
PeptideAtlasiQ8VC34.
PRIDEiQ8VC34.

PTM databases

iPTMnetiQ8VC34.
PhosphoSitePlusiQ8VC34.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033773.
CleanExiMM_RPAP2.
ExpressionAtlasiQ8VC34. baseline and differential.
GenevisibleiQ8VC34. MM.

Interactioni

Subunit structurei

Associates with the RNA polymerase II complex. Interacts with transcribing RNA polymerase II phosphorylated on 'Ser-7' on CTD (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070209.

Structurei

3D structure databases

ProteinModelPortaliQ8VC34.
SMRiQ8VC34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili41 – 68Sequence analysisAdd BLAST28

Sequence similaritiesi

Belongs to the RPAP2 family.Curated
Contains 1 RTR1-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri77 – 160RTR1-typePROSITE-ProRule annotationAdd BLAST84

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG4780. Eukaryota.
ENOG410ZZZC. LUCA.
GeneTreeiENSGT00390000017965.
HOGENOMiHOG000253960.
HOVERGENiHBG080953.
InParanoidiQ8VC34.
KOiK20827.
OMAiCYRASKY.
OrthoDBiEOG091G0TLM.
PhylomeDBiQ8VC34.
TreeFamiTF331431.

Family and domain databases

InterProiIPR007308. DUF408.
[Graphical view]
PfamiPF04181. RPAP2_Rtr1. 1 hit.
[Graphical view]
PROSITEiPS51479. ZF_RTR1. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VC34-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADSAVPCSL GPSTRASSTH RDATGTKQTR ALKRGDASKR QAELEAAIQR
60 70 80 90 100
KVEFERKAVR IVEQLLEENI TEEFLKECGM FITPAHYSDV VDERSIIKLC
110 120 130 140 150
GYPLCQKKLG VIPKQKYRIS TKTNKVYDIT ERKSFCSNFC YRASKFFETQ
160 170 180 190 200
IPKTPVWVRE EERPPDFQLL KKGQSGSSGE VVQFFRDAVT AADVDGSGAL
210 220 230 240 250
EAQCDPASSS SWSERASDEE EQGFVSSLLP GNRPKAVDTR PQPHTKSSIM
260 270 280 290 300
RKKAAQNVDS KEGEQTVSEV TEQLDNCRLD SQEKVATCKR PLKKESTQIS
310 320 330 340 350
SPGPLCDRFN TSAISEHKHG VSQVTLVGIS KKSAEHFRSK FAKSNPGSGS
360 370 380 390 400
ASGLVHVRPE VAKANLLRVL SDTLTEWKTE ETLKFLYGQN HDSVCLKPSS
410 420 430 440 450
ASEPDEELDE DDISCDPGSC GPALSQAQNT LDATLPFRGS DTAIKPLPSY
460 470 480 490 500
ESLKKETEML NLRVREFYRG RCVLNEDTTK SQDSKESVLQ RDPSFPLIDS
510 520 530 540 550
SSQNQIRRRI VLEKLSKVLP GLLGPLQITM GDIYTELKNL IQTFRLSNRN
560 570 580 590 600
IIHKPVEWTL IAVVLLLLLT PILGIQKHSP KNVVFTQFIA TLLTELHLKF
610
EDLEKLTMIF RTSC
Length:614
Mass (Da):68,530
Last modified:October 3, 2006 - v2
Checksum:i3029373A246478D7
GO
Isoform 2 (identifier: Q8VC34-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     569-570: LT → IL
     571-614: Missing.

Note: No experimental confirmation available.
Show »
Length:570
Mass (Da):63,458
Checksum:iFC6E023D8CCD2587
GO
Isoform 3 (identifier: Q8VC34-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     569-614: Missing.

Note: No experimental confirmation available.
Show »
Length:568
Mass (Da):63,232
Checksum:i423D8CCD2587F3F5
GO
Isoform 4 (identifier: Q8VC34-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     569-614: Missing.

Note: No experimental confirmation available.
Show »
Length:489
Mass (Da):54,504
Checksum:iF61E7558CBA75A2F
GO
Isoform 5 (identifier: Q8VC34-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.
     78-78: C → M

Note: No experimental confirmation available.
Show »
Length:537
Mass (Da):59,990
Checksum:i69F5ECA9B2C0AEA4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201E → D in AAH21895 (PubMed:15489334).Curated1
Sequence conflicti318K → T in AAH21895 (PubMed:15489334).Curated1
Sequence conflicti444I → V in BAE26681 (PubMed:16141072).Curated1
Sequence conflicti524G → V in BAE38864 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0206821 – 79Missing in isoform 4. 1 PublicationAdd BLAST79
Alternative sequenceiVSP_0206831 – 77Missing in isoform 5. 1 PublicationAdd BLAST77
Alternative sequenceiVSP_02068478C → M in isoform 5. 1 Publication1
Alternative sequenceiVSP_020685569 – 614Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST46
Alternative sequenceiVSP_020686569 – 570LT → IL in isoform 2. 1 Publication2
Alternative sequenceiVSP_020687571 – 614Missing in isoform 2. 1 PublicationAdd BLAST44

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034418 mRNA. Translation: BAC28703.1.
AK049889 mRNA. Translation: BAC33973.1.
AK145830 mRNA. Translation: BAE26681.1.
AK161901 mRNA. Translation: BAE36627.1.
AK166574 mRNA. Translation: BAE38864.1.
BC021895 mRNA. Translation: AAH21895.1.
CCDSiCCDS19506.1. [Q8VC34-1]
CCDS51588.1. [Q8VC34-3]
CCDS51589.1. [Q8VC34-4]
CCDS71630.1. [Q8VC34-2]
CCDS71631.1. [Q8VC34-5]
RefSeqiNP_001156933.1. NM_001163461.2. [Q8VC34-3]
NP_001156934.1. NM_001163462.2. [Q8VC34-4]
NP_001276498.1. NM_001289569.1. [Q8VC34-5]
NP_001276499.1. NM_001289570.1. [Q8VC34-2]
NP_659160.2. NM_144911.3. [Q8VC34-1]
XP_006534963.1. XM_006534900.3. [Q8VC34-5]
UniGeneiMm.482420.

Genome annotation databases

EnsembliENSMUST00000065422; ENSMUSP00000070209; ENSMUSG00000033773. [Q8VC34-1]
ENSMUST00000112650; ENSMUSP00000108269; ENSMUSG00000033773. [Q8VC34-4]
ENSMUST00000112651; ENSMUSP00000108270; ENSMUSG00000033773. [Q8VC34-5]
ENSMUST00000112654; ENSMUSP00000108273; ENSMUSG00000033773. [Q8VC34-3]
ENSMUST00000112655; ENSMUSP00000108274; ENSMUSG00000033773. [Q8VC34-2]
GeneIDi231571.
KEGGimmu:231571.
UCSCiuc008ymn.3. mouse. [Q8VC34-1]
uc008ymq.3. mouse. [Q8VC34-5]
uc033iky.1. mouse. [Q8VC34-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK034418 mRNA. Translation: BAC28703.1.
AK049889 mRNA. Translation: BAC33973.1.
AK145830 mRNA. Translation: BAE26681.1.
AK161901 mRNA. Translation: BAE36627.1.
AK166574 mRNA. Translation: BAE38864.1.
BC021895 mRNA. Translation: AAH21895.1.
CCDSiCCDS19506.1. [Q8VC34-1]
CCDS51588.1. [Q8VC34-3]
CCDS51589.1. [Q8VC34-4]
CCDS71630.1. [Q8VC34-2]
CCDS71631.1. [Q8VC34-5]
RefSeqiNP_001156933.1. NM_001163461.2. [Q8VC34-3]
NP_001156934.1. NM_001163462.2. [Q8VC34-4]
NP_001276498.1. NM_001289569.1. [Q8VC34-5]
NP_001276499.1. NM_001289570.1. [Q8VC34-2]
NP_659160.2. NM_144911.3. [Q8VC34-1]
XP_006534963.1. XM_006534900.3. [Q8VC34-5]
UniGeneiMm.482420.

3D structure databases

ProteinModelPortaliQ8VC34.
SMRiQ8VC34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070209.

PTM databases

iPTMnetiQ8VC34.
PhosphoSitePlusiQ8VC34.

Proteomic databases

PaxDbiQ8VC34.
PeptideAtlasiQ8VC34.
PRIDEiQ8VC34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065422; ENSMUSP00000070209; ENSMUSG00000033773. [Q8VC34-1]
ENSMUST00000112650; ENSMUSP00000108269; ENSMUSG00000033773. [Q8VC34-4]
ENSMUST00000112651; ENSMUSP00000108270; ENSMUSG00000033773. [Q8VC34-5]
ENSMUST00000112654; ENSMUSP00000108273; ENSMUSG00000033773. [Q8VC34-3]
ENSMUST00000112655; ENSMUSP00000108274; ENSMUSG00000033773. [Q8VC34-2]
GeneIDi231571.
KEGGimmu:231571.
UCSCiuc008ymn.3. mouse. [Q8VC34-1]
uc008ymq.3. mouse. [Q8VC34-5]
uc033iky.1. mouse. [Q8VC34-2]

Organism-specific databases

CTDi79871.
MGIiMGI:2141142. Rpap2.

Phylogenomic databases

eggNOGiKOG4780. Eukaryota.
ENOG410ZZZC. LUCA.
GeneTreeiENSGT00390000017965.
HOGENOMiHOG000253960.
HOVERGENiHBG080953.
InParanoidiQ8VC34.
KOiK20827.
OMAiCYRASKY.
OrthoDBiEOG091G0TLM.
PhylomeDBiQ8VC34.
TreeFamiTF331431.

Enzyme and pathway databases

ReactomeiR-MMU-6807505. RNA polymerase II transcribes snRNA genes.

Miscellaneous databases

ChiTaRSiRpap2. mouse.
PROiQ8VC34.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033773.
CleanExiMM_RPAP2.
ExpressionAtlasiQ8VC34. baseline and differential.
GenevisibleiQ8VC34. MM.

Family and domain databases

InterProiIPR007308. DUF408.
[Graphical view]
PfamiPF04181. RPAP2_Rtr1. 1 hit.
[Graphical view]
PROSITEiPS51479. ZF_RTR1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPAP2_MOUSE
AccessioniPrimary (citable) accession number: Q8VC34
Secondary accession number(s): Q3TLC8
, Q3TSP8, Q3UKX0, Q8C7M5, Q8CBW8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 3, 2006
Last modified: November 30, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.