Q8VC34 (RPAP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative RNA polymerase II subunit B1 CTD phosphatase Rpap2
EC=3.1.3.16

Alternative name(s):
RNA polymerase II-associated protein 2

Gene names

Name:

Rpap2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	614 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protein phosphatase that displays CTD phosphatase activity and regulates transcription of snRNA genes. Recognizes and binds phosphorylated 'Ser-7' of the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and mediates dephosphorylation of 'Ser-5' of the CTD, thereby promoting transcription of snRNA genes By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Subunit structure	Associates with the RNA polymerase II complex. Interacts with transcribing RNA polymerase II phosphorylated on 'Ser-7' on CTD By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Shuttles between the cytoplasm and the nucleus in a CRM1-dependent manner By similarity.
Sequence similarities	Belongs to the RPAP2 family. Contains 1 RTR1-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Protein phosphatase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dephosphorylation of RNA polymerase II C-terminal domain Inferred from sequence or structural similarity. Source: UniProtKB snRNA transcription Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	DNA-directed RNA polymerase II, holoenzyme Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	CTD phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VC34-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VC34-2)

The sequence of this isoform differs from the canonical sequence as follows:
     569-570: LT → IL
     571-614: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q8VC34-3)

The sequence of this isoform differs from the canonical sequence as follows:
     569-614: Missing.

Note: No experimental confirmation available.

Isoform 4 (identifier: Q8VC34-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.
     569-614: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: Q8VC34-5)

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.
     78-78: C → M

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 614	614	Putative RNA polymerase II subunit B1 CTD phosphatase Rpap2		PRO_0000250649
Regions
	Zinc finger	77 – 160	84	RTR1-type
	Coiled coil	41 – 68	28	Potential
Amino acid modifications
	Modified residue	481	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1 – 79	79	Missing in isoform 4.		VSP_020682
	Alternative sequence	1 – 77	77	Missing in isoform 5.		VSP_020683
	Alternative sequence	78	1	C → M in isoform 5.		VSP_020684
	Alternative sequence	569 – 614	46	Missing in isoform 3 and isoform 4.		VSP_020685
	Alternative sequence	569 – 570	2	LT → IL in isoform 2.		VSP_020686
	Alternative sequence	571 – 614	44	Missing in isoform 2.		VSP_020687
Experimental info
	Sequence conflict	201	1	E → D in AAH21895. Ref.2
	Sequence conflict	318	1	K → T in AAH21895. Ref.2
	Sequence conflict	444	1	I → V in BAE26681. Ref.1
	Sequence conflict	524	1	G → V in BAE38864. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: 3029373A246478D7	FASTA	614	68,530
	10 20 30 40 50 60 MADSAVPCSL GPSTRASSTH RDATGTKQTR ALKRGDASKR QAELEAAIQR KVEFERKAVR 70 80 90 100 110 120 IVEQLLEENI TEEFLKECGM FITPAHYSDV VDERSIIKLC GYPLCQKKLG VIPKQKYRIS 130 140 150 160 170 180 TKTNKVYDIT ERKSFCSNFC YRASKFFETQ IPKTPVWVRE EERPPDFQLL KKGQSGSSGE 190 200 210 220 230 240 VVQFFRDAVT AADVDGSGAL EAQCDPASSS SWSERASDEE EQGFVSSLLP GNRPKAVDTR 250 260 270 280 290 300 PQPHTKSSIM RKKAAQNVDS KEGEQTVSEV TEQLDNCRLD SQEKVATCKR PLKKESTQIS 310 320 330 340 350 360 SPGPLCDRFN TSAISEHKHG VSQVTLVGIS KKSAEHFRSK FAKSNPGSGS ASGLVHVRPE 370 380 390 400 410 420 VAKANLLRVL SDTLTEWKTE ETLKFLYGQN HDSVCLKPSS ASEPDEELDE DDISCDPGSC 430 440 450 460 470 480 GPALSQAQNT LDATLPFRGS DTAIKPLPSY ESLKKETEML NLRVREFYRG RCVLNEDTTK 490 500 510 520 530 540 SQDSKESVLQ RDPSFPLIDS SSQNQIRRRI VLEKLSKVLP GLLGPLQITM GDIYTELKNL 550 560 570 580 590 600 IQTFRLSNRN IIHKPVEWTL IAVVLLLLLT PILGIQKHSP KNVVFTQFIA TLLTELHLKF 610 EDLEKLTMIF RTSC
	Isoform 2 [UniParc]. Checksum: FC6E023D8CCD2587	FASTA	570	63,458
	10 20 30 40 50 60 MADSAVPCSL GPSTRASSTH RDATGTKQTR ALKRGDASKR QAELEAAIQR KVEFERKAVR 70 80 90 100 110 120 IVEQLLEENI TEEFLKECGM FITPAHYSDV VDERSIIKLC GYPLCQKKLG VIPKQKYRIS 130 140 150 160 170 180 TKTNKVYDIT ERKSFCSNFC YRASKFFETQ IPKTPVWVRE EERPPDFQLL KKGQSGSSGE 190 200 210 220 230 240 VVQFFRDAVT AADVDGSGAL EAQCDPASSS SWSERASDEE EQGFVSSLLP GNRPKAVDTR 250 260 270 280 290 300 PQPHTKSSIM RKKAAQNVDS KEGEQTVSEV TEQLDNCRLD SQEKVATCKR PLKKESTQIS 310 320 330 340 350 360 SPGPLCDRFN TSAISEHKHG VSQVTLVGIS KKSAEHFRSK FAKSNPGSGS ASGLVHVRPE 370 380 390 400 410 420 VAKANLLRVL SDTLTEWKTE ETLKFLYGQN HDSVCLKPSS ASEPDEELDE DDISCDPGSC 430 440 450 460 470 480 GPALSQAQNT LDATLPFRGS DTAIKPLPSY ESLKKETEML NLRVREFYRG RCVLNEDTTK 490 500 510 520 530 540 SQDSKESVLQ RDPSFPLIDS SSQNQIRRRI VLEKLSKVLP GLLGPLQITM GDIYTELKNL 550 560 570 IQTFRLSNRN IIHKPVEWTL IAVVLLLLIL
	Isoform 3 [UniParc]. Checksum: 423D8CCD2587F3F5	FASTA	568	63,232
	10 20 30 40 50 60 MADSAVPCSL GPSTRASSTH RDATGTKQTR ALKRGDASKR QAELEAAIQR KVEFERKAVR 70 80 90 100 110 120 IVEQLLEENI TEEFLKECGM FITPAHYSDV VDERSIIKLC GYPLCQKKLG VIPKQKYRIS 130 140 150 160 170 180 TKTNKVYDIT ERKSFCSNFC YRASKFFETQ IPKTPVWVRE EERPPDFQLL KKGQSGSSGE 190 200 210 220 230 240 VVQFFRDAVT AADVDGSGAL EAQCDPASSS SWSERASDEE EQGFVSSLLP GNRPKAVDTR 250 260 270 280 290 300 PQPHTKSSIM RKKAAQNVDS KEGEQTVSEV TEQLDNCRLD SQEKVATCKR PLKKESTQIS 310 320 330 340 350 360 SPGPLCDRFN TSAISEHKHG VSQVTLVGIS KKSAEHFRSK FAKSNPGSGS ASGLVHVRPE 370 380 390 400 410 420 VAKANLLRVL SDTLTEWKTE ETLKFLYGQN HDSVCLKPSS ASEPDEELDE DDISCDPGSC 430 440 450 460 470 480 GPALSQAQNT LDATLPFRGS DTAIKPLPSY ESLKKETEML NLRVREFYRG RCVLNEDTTK 490 500 510 520 530 540 SQDSKESVLQ RDPSFPLIDS SSQNQIRRRI VLEKLSKVLP GLLGPLQITM GDIYTELKNL 550 560 IQTFRLSNRN IIHKPVEWTL IAVVLLLL
	Isoform 4 [UniParc]. Checksum: F61E7558CBA75A2F	FASTA	489	54,504
	10 20 30 40 50 60 MFITPAHYSD VVDERSIIKL CGYPLCQKKL GVIPKQKYRI STKTNKVYDI TERKSFCSNF 70 80 90 100 110 120 CYRASKFFET QIPKTPVWVR EEERPPDFQL LKKGQSGSSG EVVQFFRDAV TAADVDGSGA 130 140 150 160 170 180 LEAQCDPASS SSWSERASDE EEQGFVSSLL PGNRPKAVDT RPQPHTKSSI MRKKAAQNVD 190 200 210 220 230 240 SKEGEQTVSE VTEQLDNCRL DSQEKVATCK RPLKKESTQI SSPGPLCDRF NTSAISEHKH 250 260 270 280 290 300 GVSQVTLVGI SKKSAEHFRS KFAKSNPGSG SASGLVHVRP EVAKANLLRV LSDTLTEWKT 310 320 330 340 350 360 EETLKFLYGQ NHDSVCLKPS SASEPDEELD EDDISCDPGS CGPALSQAQN TLDATLPFRG 370 380 390 400 410 420 SDTAIKPLPS YESLKKETEM LNLRVREFYR GRCVLNEDTT KSQDSKESVL QRDPSFPLID 430 440 450 460 470 480 SSSQNQIRRR IVLEKLSKVL PGLLGPLQIT MGDIYTELKN LIQTFRLSNR NIIHKPVEWT LIAVVLLLL
	Isoform 5 [UniParc]. Checksum: 69F5ECA9B2C0AEA4	FASTA	537	59,990
	10 20 30 40 50 60 MGMFITPAHY SDVVDERSII KLCGYPLCQK KLGVIPKQKY RISTKTNKVY DITERKSFCS 70 80 90 100 110 120 NFCYRASKFF ETQIPKTPVW VREEERPPDF QLLKKGQSGS SGEVVQFFRD AVTAADVDGS 130 140 150 160 170 180 GALEAQCDPA SSSSWSERAS DEEEQGFVSS LLPGNRPKAV DTRPQPHTKS SIMRKKAAQN 190 200 210 220 230 240 VDSKEGEQTV SEVTEQLDNC RLDSQEKVAT CKRPLKKEST QISSPGPLCD RFNTSAISEH 250 260 270 280 290 300 KHGVSQVTLV GISKKSAEHF RSKFAKSNPG SGSASGLVHV RPEVAKANLL RVLSDTLTEW 310 320 330 340 350 360 KTEETLKFLY GQNHDSVCLK PSSASEPDEE LDEDDISCDP GSCGPALSQA QNTLDATLPF 370 380 390 400 410 420 RGSDTAIKPL PSYESLKKET EMLNLRVREF YRGRCVLNED TTKSQDSKES VLQRDPSFPL 430 440 450 460 470 480 IDSSSQNQIR RRIVLEKLSK VLPGLLGPLQ ITMGDIYTEL KNLIQTFRLS NRNIIHKPVE 490 500 510 520 530 WTLIAVVLLL LLTPILGIQK HSPKNVVFTQ FIATLLTELH LKFEDLEKLT MIFRTSC

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3; 4 AND 5). Strain: C57BL/6J. Tissue: Diencephalon, Hippocampus, Medulla oblongata and Placenta.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J and FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK034418 mRNA. Translation: BAC28703.1. AK049889 mRNA. Translation: BAC33973.1. AK145830 mRNA. Translation: BAE26681.1. AK161901 mRNA. Translation: BAE36627.1. AK166574 mRNA. Translation: BAE38864.1. BC021895 mRNA. Translation: AAH21895.1.
IPI	IPI00121353. IPI00788458. IPI00788475. IPI00788486. IPI00788499.
RefSeq	NP_001156933.1. NM_001163461.1. NP_001156934.1. NM_001163462.1. NP_659160.2. NM_144911.2.
UniGene	Mm.482420.
3D structure databases
ProteinModelPortal	Q8VC34.
SMR	Q8VC34. Positions 53-171.
ModBase	Search...
PTM databases
PhosphoSite	Q8VC34.
Proteomic databases
PaxDb	Q8VC34.
PRIDE	Q8VC34.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000065422; ENSMUSP00000070209; ENSMUSG00000033773. ENSMUST00000112650; ENSMUSP00000108269; ENSMUSG00000033773. ENSMUST00000112651; ENSMUSP00000108270; ENSMUSG00000033773. ENSMUST00000112654; ENSMUSP00000108273; ENSMUSG00000033773. ENSMUST00000112655; ENSMUSP00000108274; ENSMUSG00000033773.
GeneID	231571.
KEGG	mmu:231571.
UCSC	uc008ymn.2. mouse. uc008ymo.2. mouse. uc008ymp.2. mouse. uc008ymq.2. mouse.
Organism-specific databases
CTD	79871.
MGI	MGI:2141142. Rpap2.
Phylogenomic databases
eggNOG	NOG241465.
GeneTree	ENSGT00390000017965.
HOGENOM	HOG000253960.
HOVERGEN	HBG080953.
InParanoid	Q8VC34.
OMA	ECGKFIT.
Gene expression databases
ArrayExpress	Q8VC34.
Bgee	Q8VC34.
CleanEx	MM_RPAP2.
Genevestigator	Q8VC34.
GermOnline	ENSMUSG00000033773. Mus musculus.
Family and domain databases
InterPro	IPR007308. DUF408. [Graphical view]
Pfam	PF04181. RPAP2_Rtr1. 1 hit. [Graphical view]
PROSITE	PS51479. ZF_RTR1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	RPAP2. mouse.
NextBio	380622.
SOURCE	Search...

Entry information

Entry name

RPAP2_MOUSE

Accession

Primary (citable) accession number: Q8VC34
Secondary accession number(s): Q3TLC8 , Q3TSP8, Q3UKX0, Q8C7M5, Q8CBW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program