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Q8VC30

- DHAK_MOUSE

UniProt

Q8VC30 - DHAK_MOUSE

Protein

Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)

Gene

Dak

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.By similarity

    Catalytic activityi

    ATP + glycerone = ADP + glycerone phosphate.
    ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.
    FAD = AMP + riboflavin cyclic-4',5'-phosphate.

    Cofactori

    Magnesium.By similarity
    Manganese or cobalt; for FAD-AMP lyase activity.By similarity

    Enzyme regulationi

    Each activity is inhibited by the substrate(s) of the other.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091DihydroxyacetonePROSITE-ProRule annotation
    Binding sitei114 – 1141DihydroxyacetonePROSITE-ProRule annotation
    Active sitei221 – 2211Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation
    Binding sitei486 – 4861ATP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi401 – 4044ATPBy similarity
    Nucleotide bindingi446 – 4472ATPBy similarity
    Nucleotide bindingi494 – 4952ATPBy similarity
    Nucleotide bindingi556 – 5583ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. FAD-AMP lyase (cyclizing) activity Source: UniProtKB-EC
    3. glycerone kinase activity Source: UniProtKB-EC
    4. metal ion binding Source: UniProtKB-KW
    5. protein binding Source: MGI
    6. triokinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycerol metabolic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Lyase, Transferase

    Keywords - Ligandi

    ATP-binding, Cobalt, FAD, Flavoprotein, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
    Including the following 2 domains:
    ATP-dependent dihydroxyacetone kinase (EC:2.7.1.28, EC:2.7.1.29)
    Short name:
    DHA kinase
    Alternative name(s):
    Glycerone kinase
    Triokinase
    Triose kinase
    FAD-AMP lyase (cyclizing) (EC:4.6.1.15)
    Alternative name(s):
    FAD-AMP lyase (cyclic FMN forming)
    FMN cyclase
    Gene namesi
    Name:Dak
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:2385084. Dak.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 578578Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)PRO_0000121526Add
    BLAST

    Proteomic databases

    MaxQBiQ8VC30.
    PaxDbiQ8VC30.
    PRIDEiQ8VC30.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00310669.
    Q8VC30.

    PTM databases

    PhosphoSiteiQ8VC30.

    Expressioni

    Gene expression databases

    BgeeiQ8VC30.
    CleanExiMM_DAK.
    GenevestigatoriQ8VC30.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiQ8VC30. 5 interactions.
    MINTiMINT-1869666.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VC30.
    SMRiQ8VC30. Positions 3-571.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini9 – 336328DhaKPROSITE-ProRule annotationAdd
    BLAST
    Domaini372 – 571200DhaLPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni56 – 594Dihydroxyacetone bindingBy similarity

    Sequence similaritiesi

    Contains 1 DhaK domain.PROSITE-ProRule annotation
    Contains 1 DhaL domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2376.
    GeneTreeiENSGT00390000015415.
    HOGENOMiHOG000234158.
    HOVERGENiHBG079502.
    InParanoidiQ8VC30.
    KOiK00863.
    OMAiDHMTDPS.
    OrthoDBiEOG71K630.
    PhylomeDBiQ8VC30.
    TreeFamiTF313821.

    Family and domain databases

    InterProiIPR004006. Dak1.
    IPR012734. DhaK_ATP.
    IPR004007. DhaL_dom.
    [Graphical view]
    PfamiPF02733. Dak1. 1 hit.
    PF02734. Dak2. 1 hit.
    [Graphical view]
    SUPFAMiSSF101473. SSF101473. 1 hit.
    TIGRFAMsiTIGR02361. dak_ATP. 1 hit.
    PROSITEiPS51481. DHAK. 1 hit.
    PS51480. DHAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VC30-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSKKMVNSV EGCADDALAG LVASNPDLQL LQGHRVALRS DLDTLKGRVA    50
    LLSGGGSGHE PAHAGFIGKG MLTGVIAGSV FASPPVGSIL AAIRAVAQAG 100
    TVGTLLIVKN YTGDRLNFGL AMEQAKAEGI SVEMVIVEDD SAFTVLKKAG 150
    RRGLCGTVLI HKVAGALAEE GMGLEEITKR VSVIAKTMGT LGVSLSSCSV 200
    PGATHTFELA ADEIELGLGI HGEAGVRRIK IAPVDQIVTL MLDHMTNTSN 250
    IFHVPVRSGS SVVLIVNNLG GLSFLELGII ADAAIRLLEG RGVKVARALV 300
    GTFMSALEMP GVSLTLMLVD EPVLKLIDAE TTAKAWPHMA KVSVTGRKRI 350
    RAAPTEPPEA PEATAAGGVT SKQMALVLDR ICTTLIGLEE HLNALDRAAG 400
    DGDCGSTHSR AAKAIQGWLK EGPSLTSPAQ VLSRLSVLLL ERMGGSSGAL 450
    YGLFLTAAAQ PLKAKTDLPT WSAAMDAGLE SMQKYGKAAP GDRTMLDSLW 500
    AAAQEFQAWK SPGASLLPVL TKAVKSAEAA AEATKNMEAG AGRASYISSA 550
    QLDQPDPGAV AAAAIFRAIL EVLQTQGA 578
    Length:578
    Mass (Da):59,691
    Last modified:March 1, 2002 - v1
    Checksum:i5C6FCABDD6F2EF2A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC021917 mRNA. Translation: AAH21917.1.
    CCDSiCCDS37914.1.
    RefSeqiNP_663471.1. NM_145496.1.
    XP_006527005.1. XM_006526942.1.
    XP_006527006.1. XM_006526943.1.
    XP_006527007.1. XM_006526944.1.
    UniGeneiMm.374868.

    Genome annotation databases

    EnsembliENSMUST00000037678; ENSMUSP00000044556; ENSMUSG00000034371.
    GeneIDi225913.
    KEGGimmu:225913.
    UCSCiuc008gql.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC021917 mRNA. Translation: AAH21917.1 .
    CCDSi CCDS37914.1.
    RefSeqi NP_663471.1. NM_145496.1.
    XP_006527005.1. XM_006526942.1.
    XP_006527006.1. XM_006526943.1.
    XP_006527007.1. XM_006526944.1.
    UniGenei Mm.374868.

    3D structure databases

    ProteinModelPortali Q8VC30.
    SMRi Q8VC30. Positions 3-571.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8VC30. 5 interactions.
    MINTi MINT-1869666.

    PTM databases

    PhosphoSitei Q8VC30.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00310669.
    Q8VC30.

    Proteomic databases

    MaxQBi Q8VC30.
    PaxDbi Q8VC30.
    PRIDEi Q8VC30.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037678 ; ENSMUSP00000044556 ; ENSMUSG00000034371 .
    GeneIDi 225913.
    KEGGi mmu:225913.
    UCSCi uc008gql.1. mouse.

    Organism-specific databases

    CTDi 26007.
    MGIi MGI:2385084. Dak.

    Phylogenomic databases

    eggNOGi COG2376.
    GeneTreei ENSGT00390000015415.
    HOGENOMi HOG000234158.
    HOVERGENi HBG079502.
    InParanoidi Q8VC30.
    KOi K00863.
    OMAi DHMTDPS.
    OrthoDBi EOG71K630.
    PhylomeDBi Q8VC30.
    TreeFami TF313821.

    Miscellaneous databases

    NextBioi 377881.
    PROi Q8VC30.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8VC30.
    CleanExi MM_DAK.
    Genevestigatori Q8VC30.

    Family and domain databases

    InterProi IPR004006. Dak1.
    IPR012734. DhaK_ATP.
    IPR004007. DhaL_dom.
    [Graphical view ]
    Pfami PF02733. Dak1. 1 hit.
    PF02734. Dak2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101473. SSF101473. 1 hit.
    TIGRFAMsi TIGR02361. dak_ATP. 1 hit.
    PROSITEi PS51481. DHAK. 1 hit.
    PS51480. DHAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Eye.

    Entry informationi

    Entry nameiDHAK_MOUSE
    AccessioniPrimary (citable) accession number: Q8VC30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3