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Protein

Triokinase/FMN cyclase

Gene

Tkfc

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response.By similarity

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.
FAD = AMP + riboflavin cyclic-4',5'-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • Mn2+By similarity, Co2+By similarityNote: Manganese or cobalt are requested for FAD-AMP lyase activity.By similarity

Enzyme regulationi

Each activity is inhibited by the substrate(s) of the other.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109DihydroxyacetonePROSITE-ProRule annotation1
Binding sitei114DihydroxyacetonePROSITE-ProRule annotation1
Active sitei221Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation1
Binding sitei486ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi401 – 404ATPBy similarity4
Nucleotide bindingi446 – 447ATPBy similarity2
Nucleotide bindingi494 – 495ATPBy similarity2
Nucleotide bindingi556 – 558ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Lyase, Multifunctional enzyme, Transferase
LigandATP-binding, Cobalt, FAD, Flavoprotein, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-70350 Fructose catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Triokinase/FMN cyclaseImported
Alternative name(s):
Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
Including the following 2 domains:
ATP-dependent dihydroxyacetone kinase (EC:2.7.1.28, EC:2.7.1.29)
Short name:
DHA kinase
Alternative name(s):
Glycerone kinase
Triokinase
Triose kinase
FAD-AMP lyase (cyclizing) (EC:4.6.1.15)
Alternative name(s):
FAD-AMP lyase (cyclic FMN forming)
FMN cyclase
Gene namesi
Name:TkfcImported
Synonyms:DakImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2385084 Tkfc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001215261 – 578Triokinase/FMN cyclaseAdd BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei511PhosphoserineCombined sources1
Modified residuei545PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VC30
MaxQBiQ8VC30
PaxDbiQ8VC30
PeptideAtlasiQ8VC30
PRIDEiQ8VC30

2D gel databases

REPRODUCTION-2DPAGEiIPI00310669
Q8VC30

PTM databases

iPTMnetiQ8VC30
PhosphoSitePlusiQ8VC30
SwissPalmiQ8VC30

Expressioni

Gene expression databases

BgeeiENSMUSG00000034371
CleanExiMM_DAK
GenevisibleiQ8VC30 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with IFIH1 (via the CARD domains), the interaction is inhibited by viral infection (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8VC30, 5 interactors
MINTiQ8VC30
STRINGi10090.ENSMUSP00000044556

Structurei

3D structure databases

ProteinModelPortaliQ8VC30
SMRiQ8VC30
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 336DhaKPROSITE-ProRule annotationAdd BLAST328
Domaini372 – 571DhaLPROSITE-ProRule annotationAdd BLAST200

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 59Dihydroxyacetone bindingBy similarity4

Domaini

DhaK and DhaL domains have differential roles, individually DhaK is inactive and DhaL displays cyclase but not kinase activity.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2426 Eukaryota
COG2376 LUCA
GeneTreeiENSGT00390000015415
HOGENOMiHOG000234158
HOVERGENiHBG079502
InParanoidiQ8VC30
KOiK00863
OMAiAWPNVAK
OrthoDBiEOG091G0P13
PhylomeDBiQ8VC30
TreeFamiTF313821

Family and domain databases

Gene3Di1.25.40.340, 1 hit
InterProiView protein in InterPro
IPR012734 DhaK_ATP
IPR004006 DhaK_dom
IPR004007 DhaL_dom
IPR036117 DhaL_dom_sf
PfamiView protein in Pfam
PF02733 Dak1, 1 hit
PF02734 Dak2, 1 hit
SMARTiView protein in SMART
SM01120 Dak2, 1 hit
SUPFAMiSSF101473 SSF101473, 1 hit
TIGRFAMsiTIGR02361 dak_ATP, 1 hit
PROSITEiView protein in PROSITE
PS51481 DHAK, 1 hit
PS51480 DHAL, 1 hit

Sequencei

Sequence statusi: Complete.

Q8VC30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKKMVNSV EGCADDALAG LVASNPDLQL LQGHRVALRS DLDTLKGRVA
60 70 80 90 100
LLSGGGSGHE PAHAGFIGKG MLTGVIAGSV FASPPVGSIL AAIRAVAQAG
110 120 130 140 150
TVGTLLIVKN YTGDRLNFGL AMEQAKAEGI SVEMVIVEDD SAFTVLKKAG
160 170 180 190 200
RRGLCGTVLI HKVAGALAEE GMGLEEITKR VSVIAKTMGT LGVSLSSCSV
210 220 230 240 250
PGATHTFELA ADEIELGLGI HGEAGVRRIK IAPVDQIVTL MLDHMTNTSN
260 270 280 290 300
IFHVPVRSGS SVVLIVNNLG GLSFLELGII ADAAIRLLEG RGVKVARALV
310 320 330 340 350
GTFMSALEMP GVSLTLMLVD EPVLKLIDAE TTAKAWPHMA KVSVTGRKRI
360 370 380 390 400
RAAPTEPPEA PEATAAGGVT SKQMALVLDR ICTTLIGLEE HLNALDRAAG
410 420 430 440 450
DGDCGSTHSR AAKAIQGWLK EGPSLTSPAQ VLSRLSVLLL ERMGGSSGAL
460 470 480 490 500
YGLFLTAAAQ PLKAKTDLPT WSAAMDAGLE SMQKYGKAAP GDRTMLDSLW
510 520 530 540 550
AAAQEFQAWK SPGASLLPVL TKAVKSAEAA AEATKNMEAG AGRASYISSA
560 570
QLDQPDPGAV AAAAIFRAIL EVLQTQGA
Length:578
Mass (Da):59,691
Last modified:March 1, 2002 - v1
Checksum:i5C6FCABDD6F2EF2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC021917 mRNA Translation: AAH21917.1
CCDSiCCDS37914.1
RefSeqiNP_663471.1, NM_145496.1
XP_006527005.1, XM_006526942.2
XP_006527006.1, XM_006526943.3
XP_006527007.1, XM_006526944.3
UniGeneiMm.374868

Genome annotation databases

EnsembliENSMUST00000037678; ENSMUSP00000044556; ENSMUSG00000034371
GeneIDi225913
KEGGimmu:225913
UCSCiuc008gql.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTKFC_MOUSE
AccessioniPrimary (citable) accession number: Q8VC30
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health