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Protein

Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)

Gene

Dak

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.By similarity

Catalytic activityi

ATP + glycerone = ADP + glycerone phosphate.
ATP + D-glyceraldehyde = ADP + D-glyceraldehyde 3-phosphate.
FAD = AMP + riboflavin cyclic-4',5'-phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • Mn2+By similarity, Co2+By similarityNote: Manganese or cobalt are requested for FAD-AMP lyase activity.By similarity

Enzyme regulationi

Each activity is inhibited by the substrate(s) of the other.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091DihydroxyacetonePROSITE-ProRule annotation
Binding sitei114 – 1141DihydroxyacetonePROSITE-ProRule annotation
Active sitei221 – 2211Tele-hemiaminal-histidine intermediatePROSITE-ProRule annotation
Binding sitei486 – 4861ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi401 – 4044ATPBy similarity
Nucleotide bindingi446 – 4472ATPBy similarity
Nucleotide bindingi494 – 4952ATPBy similarity
Nucleotide bindingi556 – 5583ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Lyase, Transferase

Keywords - Ligandi

ATP-binding, Cobalt, FAD, Flavoprotein, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_316033. Fructose catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)
Including the following 2 domains:
ATP-dependent dihydroxyacetone kinase (EC:2.7.1.28, EC:2.7.1.29)
Short name:
DHA kinase
Alternative name(s):
Glycerone kinase
Triokinase
Triose kinase
FAD-AMP lyase (cyclizing) (EC:4.6.1.15)
Alternative name(s):
FAD-AMP lyase (cyclic FMN forming)
FMN cyclase
Gene namesi
Name:Dak
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:2385084. Dak.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase (cyclizing)PRO_0000121526Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei511 – 5111PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8VC30.
PaxDbiQ8VC30.
PRIDEiQ8VC30.

2D gel databases

REPRODUCTION-2DPAGEIPI00310669.
Q8VC30.

PTM databases

PhosphoSiteiQ8VC30.

Expressioni

Gene expression databases

BgeeiQ8VC30.
CleanExiMM_DAK.
GenevisibleiQ8VC30. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ8VC30. 5 interactions.
MINTiMINT-1869666.
STRINGi10090.ENSMUSP00000044556.

Structurei

3D structure databases

ProteinModelPortaliQ8VC30.
SMRiQ8VC30. Positions 3-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 336328DhaKPROSITE-ProRule annotationAdd
BLAST
Domaini372 – 571200DhaLPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni56 – 594Dihydroxyacetone bindingBy similarity

Sequence similaritiesi

Contains 1 DhaK domain.PROSITE-ProRule annotation
Contains 1 DhaL domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2376.
GeneTreeiENSGT00390000015415.
HOGENOMiHOG000234158.
HOVERGENiHBG079502.
InParanoidiQ8VC30.
KOiK00863.
OMAiVEMVIVA.
OrthoDBiEOG71K630.
PhylomeDBiQ8VC30.
TreeFamiTF313821.

Family and domain databases

InterProiIPR004006. Dak1.
IPR012734. DhaK_ATP.
IPR004007. DhaL_dom.
[Graphical view]
PfamiPF02733. Dak1. 1 hit.
PF02734. Dak2. 1 hit.
[Graphical view]
SUPFAMiSSF101473. SSF101473. 1 hit.
TIGRFAMsiTIGR02361. dak_ATP. 1 hit.
PROSITEiPS51481. DHAK. 1 hit.
PS51480. DHAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VC30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKKMVNSV EGCADDALAG LVASNPDLQL LQGHRVALRS DLDTLKGRVA
60 70 80 90 100
LLSGGGSGHE PAHAGFIGKG MLTGVIAGSV FASPPVGSIL AAIRAVAQAG
110 120 130 140 150
TVGTLLIVKN YTGDRLNFGL AMEQAKAEGI SVEMVIVEDD SAFTVLKKAG
160 170 180 190 200
RRGLCGTVLI HKVAGALAEE GMGLEEITKR VSVIAKTMGT LGVSLSSCSV
210 220 230 240 250
PGATHTFELA ADEIELGLGI HGEAGVRRIK IAPVDQIVTL MLDHMTNTSN
260 270 280 290 300
IFHVPVRSGS SVVLIVNNLG GLSFLELGII ADAAIRLLEG RGVKVARALV
310 320 330 340 350
GTFMSALEMP GVSLTLMLVD EPVLKLIDAE TTAKAWPHMA KVSVTGRKRI
360 370 380 390 400
RAAPTEPPEA PEATAAGGVT SKQMALVLDR ICTTLIGLEE HLNALDRAAG
410 420 430 440 450
DGDCGSTHSR AAKAIQGWLK EGPSLTSPAQ VLSRLSVLLL ERMGGSSGAL
460 470 480 490 500
YGLFLTAAAQ PLKAKTDLPT WSAAMDAGLE SMQKYGKAAP GDRTMLDSLW
510 520 530 540 550
AAAQEFQAWK SPGASLLPVL TKAVKSAEAA AEATKNMEAG AGRASYISSA
560 570
QLDQPDPGAV AAAAIFRAIL EVLQTQGA
Length:578
Mass (Da):59,691
Last modified:March 1, 2002 - v1
Checksum:i5C6FCABDD6F2EF2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC021917 mRNA. Translation: AAH21917.1.
CCDSiCCDS37914.1.
RefSeqiNP_663471.1. NM_145496.1.
XP_006527005.1. XM_006526942.2.
XP_006527006.1. XM_006526943.2.
XP_006527007.1. XM_006526944.2.
UniGeneiMm.374868.

Genome annotation databases

EnsembliENSMUST00000037678; ENSMUSP00000044556; ENSMUSG00000034371.
GeneIDi225913.
KEGGimmu:225913.
UCSCiuc008gql.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC021917 mRNA. Translation: AAH21917.1.
CCDSiCCDS37914.1.
RefSeqiNP_663471.1. NM_145496.1.
XP_006527005.1. XM_006526942.2.
XP_006527006.1. XM_006526943.2.
XP_006527007.1. XM_006526944.2.
UniGeneiMm.374868.

3D structure databases

ProteinModelPortaliQ8VC30.
SMRiQ8VC30. Positions 3-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VC30. 5 interactions.
MINTiMINT-1869666.
STRINGi10090.ENSMUSP00000044556.

PTM databases

PhosphoSiteiQ8VC30.

2D gel databases

REPRODUCTION-2DPAGEIPI00310669.
Q8VC30.

Proteomic databases

MaxQBiQ8VC30.
PaxDbiQ8VC30.
PRIDEiQ8VC30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037678; ENSMUSP00000044556; ENSMUSG00000034371.
GeneIDi225913.
KEGGimmu:225913.
UCSCiuc008gql.1. mouse.

Organism-specific databases

CTDi225913.
MGIiMGI:2385084. Dak.

Phylogenomic databases

eggNOGiCOG2376.
GeneTreeiENSGT00390000015415.
HOGENOMiHOG000234158.
HOVERGENiHBG079502.
InParanoidiQ8VC30.
KOiK00863.
OMAiVEMVIVA.
OrthoDBiEOG71K630.
PhylomeDBiQ8VC30.
TreeFamiTF313821.

Enzyme and pathway databases

ReactomeiREACT_316033. Fructose catabolism.

Miscellaneous databases

ChiTaRSiDak. mouse.
NextBioi377881.
PROiQ8VC30.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VC30.
CleanExiMM_DAK.
GenevisibleiQ8VC30. MM.

Family and domain databases

InterProiIPR004006. Dak1.
IPR012734. DhaK_ATP.
IPR004007. DhaL_dom.
[Graphical view]
PfamiPF02733. Dak1. 1 hit.
PF02734. Dak2. 1 hit.
[Graphical view]
SUPFAMiSSF101473. SSF101473. 1 hit.
TIGRFAMsiTIGR02361. dak_ATP. 1 hit.
PROSITEiPS51481. DHAK. 1 hit.
PS51480. DHAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.

Entry informationi

Entry nameiDHAK_MOUSE
AccessioniPrimary (citable) accession number: Q8VC30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.