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Protein

Calcium/calmodulin-dependent protein kinase kinase 1

Gene

Camkk1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-dependent protein kinase that belongs to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1, CAMK1D, CAMK1G and CAMK4. Involved in regulating cell apoptosis. Promotes cell survival by phosphorylating AKT1/PKB that inhibits pro-apoptotic BAD/Bcl2-antagonist of cell death.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by Ca2+/calmodulin. Binding of calmodulin may relieve intrasteric autoinhibition. Partially inhibited upon phosphorylation by PRCAKA/PKA (By similarity). May be regulated through phosphorylation by CAMK1 and CAMK4.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei157 – 1571ATPPROSITE-ProRule annotation
Active sitei275 – 2751Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1429ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calmodulin-dependent protein kinase activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-442717. CREB phosphorylation through the activation of CaMKK.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase kinase 1 (EC:2.7.11.17)
Short name:
CaM-KK 1
Short name:
CaM-kinase kinase 1
Short name:
CaMKK 1
Alternative name(s):
CaM-kinase IV kinase
Calcium/calmodulin-dependent protein kinase kinase alpha
Short name:
CaM-KK alpha
Short name:
CaM-kinase kinase alpha
Short name:
CaMKK alpha
Gene namesi
Name:Camkk1
Synonyms:Camkk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1891766. Camkk1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Calcium/calmodulin-dependent protein kinase kinase 1PRO_0000086142Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671PhosphoserineBy similarity
Modified residuei74 – 741PhosphoserineCombined sources
Modified residuei100 – 1001PhosphoserineCombined sources
Modified residuei108 – 1081PhosphothreonineBy similarity
Modified residuei458 – 4581PhosphoserineCombined sources
Modified residuei475 – 4751PhosphoserineBy similarity
Modified residuei492 – 4921PhosphoserineCombined sources

Post-translational modificationi

Appears to be autophosphorylated in a Ca2+/calmodulin-dependent manner. Phosphorylated at multiple sites by PRCAKA/PKA. Phosphorylation of Ser-458 is blocked upon binding to Ca2+/calmodulin. In vitro, phosphorylated by CAMK1 and CAMK4 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VBY2.
MaxQBiQ8VBY2.
PaxDbiQ8VBY2.
PeptideAtlasiQ8VBY2.
PRIDEiQ8VBY2.

PTM databases

iPTMnetiQ8VBY2.
PhosphoSiteiQ8VBY2.

Expressioni

Tissue specificityi

Widely expressed. Differentially expressed in various brain regions.1 Publication

Gene expression databases

BgeeiENSMUSG00000020785.
ExpressionAtlasiQ8VBY2. baseline and differential.
GenevisibleiQ8VBY2. MM.

Interactioni

Subunit structurei

Interacts with CAMK4 and calmodulin.By similarity

Protein-protein interaction databases

MINTiMINT-102795.
STRINGi10090.ENSMUSP00000090613.

Structurei

3D structure databases

ProteinModelPortaliQ8VBY2.
SMRiQ8VBY2. Positions 123-429, 438-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini128 – 409282Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni167 – 18923RP domainAdd
BLAST
Regioni435 – 4406Autoinhibitory domainBy similarity
Regioni438 – 46326Calmodulin-bindingBy similarityAdd
BLAST

Domaini

The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.
The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0585. Eukaryota.
ENOG410YHHF. LUCA.
GeneTreeiENSGT00530000063214.
HOGENOMiHOG000007846.
HOVERGENiHBG052262.
InParanoidiQ8VBY2.
KOiK07359.
OMAiVRLIPSW.
OrthoDBiEOG091G01DB.
PhylomeDBiQ8VBY2.
TreeFamiTF313013.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VBY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGPAVCCQ DPRAELVDRV AAINVAHLEE ADEGPEPARN GVDPPPRARA
60 70 80 90 100
ASVIPGSASR PTPVRPSLSA RKFSLQERPA GSCLGAQVGP YSTGPASHIS
110 120 130 140 150
PRSWRRPTIE SHRVAISDTE DCVQLNQYKL QSEIGKGAYG VVRLAYNESE
160 170 180 190 200
DRHYAMKVLS KKKLLKQYGF PRRPPPRGSQ ATQGGPAKQL LPLERVYQEI
210 220 230 240 250
AILKKLDHVN VVKLIEVLDD PAEDNLYLVF DLLRKGPVME VPCDKPFPEE
260 270 280 290 300
QARLYLRDII LGLEYLHCQK IVHRDIKPSN LLLGDDGHVK IADFGVSNQF
310 320 330 340 350
EGNDAQLSST AGTPAFMAPE AISDSGQSFS GKALDVWATG VTLYCFVYGK
360 370 380 390 400
CPFIDDYILT LHRKIKNEAV VFPEEPEVSE DLKDLILRML DKNPETRIGV
410 420 430 440 450
SDIKLHPWVT KHGEEPLPSE EEHCSVVEVT EEEVKNSVRL IPSWTTVILV
460 470 480 490 500
KSMLRKRSFG NPFEPQARRE ERSMSAPGSL LMKEGCGEGC KSPELPGVQE

DEAAS
Length:505
Mass (Da):55,838
Last modified:March 1, 2002 - v1
Checksum:i179915A25628F9C4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti99 – 1002IS → MC in AAF08348 (PubMed:10560916).Curated
Sequence conflicti198 – 1992QE → HD in AAF08348 (PubMed:10560916).Curated
Sequence conflicti382 – 3821L → S in AAF08348 (PubMed:10560916).Curated
Sequence conflicti417 – 4171L → I in AAF08348 (PubMed:10560916).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117384 mRNA. Translation: AAF08348.1.
AF461706
, AF461702, AF461703, AF461705, AF461704 Genomic DNA. Translation: AAL67849.1.
BC017529 mRNA. Translation: AAH17529.1.
CCDSiCCDS24993.1.
RefSeqiNP_061371.2. NM_018883.2.
XP_006533823.1. XM_006533760.2.
XP_006533824.1. XM_006533761.2.
XP_006536363.1. XM_006536300.2.
XP_006536364.1. XM_006536301.2.
UniGeneiMm.9998.

Genome annotation databases

EnsembliENSMUST00000092937; ENSMUSP00000090613; ENSMUSG00000020785.
GeneIDi55984.
KEGGimmu:55984.
UCSCiuc007jzt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117384 mRNA. Translation: AAF08348.1.
AF461706
, AF461702, AF461703, AF461705, AF461704 Genomic DNA. Translation: AAL67849.1.
BC017529 mRNA. Translation: AAH17529.1.
CCDSiCCDS24993.1.
RefSeqiNP_061371.2. NM_018883.2.
XP_006533823.1. XM_006533760.2.
XP_006533824.1. XM_006533761.2.
XP_006536363.1. XM_006536300.2.
XP_006536364.1. XM_006536301.2.
UniGeneiMm.9998.

3D structure databases

ProteinModelPortaliQ8VBY2.
SMRiQ8VBY2. Positions 123-429, 438-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-102795.
STRINGi10090.ENSMUSP00000090613.

PTM databases

iPTMnetiQ8VBY2.
PhosphoSiteiQ8VBY2.

Proteomic databases

EPDiQ8VBY2.
MaxQBiQ8VBY2.
PaxDbiQ8VBY2.
PeptideAtlasiQ8VBY2.
PRIDEiQ8VBY2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092937; ENSMUSP00000090613; ENSMUSG00000020785.
GeneIDi55984.
KEGGimmu:55984.
UCSCiuc007jzt.1. mouse.

Organism-specific databases

CTDi84254.
MGIiMGI:1891766. Camkk1.

Phylogenomic databases

eggNOGiKOG0585. Eukaryota.
ENOG410YHHF. LUCA.
GeneTreeiENSGT00530000063214.
HOGENOMiHOG000007846.
HOVERGENiHBG052262.
InParanoidiQ8VBY2.
KOiK07359.
OMAiVRLIPSW.
OrthoDBiEOG091G01DB.
PhylomeDBiQ8VBY2.
TreeFamiTF313013.

Enzyme and pathway databases

ReactomeiR-MMU-442717. CREB phosphorylation through the activation of CaMKK.

Miscellaneous databases

PROiQ8VBY2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020785.
ExpressionAtlasiQ8VBY2. baseline and differential.
GenevisibleiQ8VBY2. MM.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKKCC1_MOUSE
AccessioniPrimary (citable) accession number: Q8VBY2
Secondary accession number(s): Q9R054
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2002
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.