Reviewed, UniProtKB/Swiss-Prot Q8VBX1 (LIPE_RAT)

Names and origin

Protein names

Recommended name:
    Endothelial lipase
    EC=3.1.1.3
Alternative name(s):
    Endothelial-derived lipase
      Short name=EDL

Gene names

Name:

Lipg

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subunit structure	Homodimer. Interacts with apolipoprotein C-2 By similarity.
Subcellular location	Secreted By similarity.
Miscellaneous	It is termed endothelial lipase due to the fact that it is synthesized in endothelial cells, a characteristic that distinguishes it from other members of the family. However, this protein is also expressed in other cell types.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Heparin-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	cell proliferation Inferred from direct assay. Source: RGD lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to nutrient Inferred from expression pattern. Source: RGD
Cellular component	extracellular space Inferred from direct assay. Source: RGD
Molecular function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW lipoprotein lipase activity Inferred from electronic annotation. Source: InterPro triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 493	470	Endothelial lipase		PRO_0000043409
Regions
	Domain	349 – 484	136	PLAT
	Region	327 – 339	13	Heparin-binding By similarity
Sites
	Active site	171	1	Nucleophile By similarity
	Active site	195	1	Charge relay system By similarity
	Active site	276	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	395	1	N-linked (GlcNAc...) Potential
	Disulfide bond	66 ↔ 79		By similarity
	Disulfide bond	254 ↔ 274		By similarity
	Disulfide bond	299 ↔ 318		By similarity
	Disulfide bond	310 ↔ 313		By similarity
	Disulfide bond	465 ↔ 485		By similarity
Experimental info
	Sequence conflict	275 – 277	3	EHE → KHK in AAK14774. Ref.2
	Sequence conflict	275 – 277	3	EHE → KHK in AAK14775. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8VBX1-1 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 11F8E39B2E2FCBC5	FASTA	493	55,924
	10 20 30 40 50 60 MRDPVFLLGF WSLYCCFPAG SLTTLRPQGS LRDEHHKPTG VPVTITTKPS VTFNIRTSKD 70 80 90 100 110 120 PEHEGCNLSL GDSKLLENCG FNMTAKTFFI IHGWTMSGMF ESWLHKLVSA LQTREKEANV 130 140 150 160 170 180 VVVDWLPLAH QLYIDAVSNT RVVGRRVAGM LNWLQEKGEF SLGDVHLIGY SLGAHVAGYA 190 200 210 220 230 240 GNFVKGTVGR ITGLDPAGPM FEGVDINRRL SPDDADFVDV LHTYTLSFGL SIGIRMPVGH 250 260 270 280 290 300 IDIYPNGGDF QPGCGFNDVM GSFAYGTISE MVKCEHERAV HLFVDSLVNQ DKPSFAFQCT 310 320 330 340 350 360 DPNRFKRGIC LSCRKNRCNN IGYNAKKMRK KRNSKMYLKT RAGMPFRVYH YQLKVHMFSY 370 380 390 400 410 420 KNSGDIQPDL YITLYGSNAD SQNLPLEIVE KIELNATNTF LVYTEEYLGD LFKIRLTWEG 430 440 450 460 470 480 VSSSWYNLWN EFRSYLSQPS SPSRELHIRR IRVKSGETQR KVAFCVQDPM KNSISPGQEL 490 WFYKCQNDCR VKN

References

[1]	"Increased expression of endothelial lipase in rat models of hypertension." Shimokawa Y., Hirata K., Ishida T., Kojima Y., Inoue N., Quertermous T., Yokoyama M. Cardiovasc. Res. 66:594-600(2005) [PubMed: 15914124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Aortic smooth muscle.
[2]	"Sequencing and chromosomal assignment of the rat endothelial-derived lipase gene (Lipg)." Bonne A.C.M., den Bieman M.G., van Lith H., van Zutphen B.F.M. DNA Seq. 12:285-287(2001) [PubMed: 11924532] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 214-293. Strain: BN-Lx/Cub and SHR/OlaIpcv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY916123 mRNA. Translation: AAX11354.1. AY027561 Genomic DNA. Translation: AAK14774.1. AY027562 Genomic DNA. Translation: AAK14775.1.
IPI	IPI00555274.
RefSeq	NP_001012759.1.
UniGene	Rn.199051
3D structure databases
HSSP	HSSP built from PDB template 1LPB based on UniProtKB P16233.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8VBX1.
Genome annotation databases
Ensembl	ENSRNOT00000025257; ENSRNOP00000025257; ENSRNOG00000018694; Rattus norvegicus. [Genome view]
GeneID	291437.
KEGG	rno:291437.
NMPDR	fig|10116.3.peg.14388.
UCSC	NM_001012741. rat.
Organism-specific databases
CTD	291437.
RGD	1310740. Lipg.
Phylogenomic databases
HOVERGEN	Q8VBX1.
OMA	SQSWYNL.
Enzyme and pathway databases
BRENDA	3.1.1.3. 248.
Gene expression databases
ArrayExpress	Q8VBX1.
Genevestigator	Q8VBX1.
GermOnline	ENSRNOG00000018694. Rattus norvegicus.
Family and domain databases
InterPro	IPR000734. Lipase. IPR013818. Lipase_N. IPR008262. Lipase_Ser_AS. IPR002330. Lipo_Lipase. IPR001024. LipOase_LH2. IPR016272. Lipoprotein_lipase_LIPH. [Graphical view]
PANTHER	PTHR11610. Lipase. 1 hit.
Pfam	PF00151. Lipase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PIRSF	PIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTS	PR00822. LIPOLIPASE. PR00821. TAGLIPASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	632583.

Entry information

Entry name

LIPE_RAT

Accession

Primary (citable) accession number: Q8VBX1
Secondary accession number(s): Q5D216

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	December 6, 2005
Last modified:	November 3, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)