Q8VBW6 (ULA1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NEDD8-activating enzyme E1 regulatory subunit

Alternative name(s):
Amyloid beta precursor protein-binding protein 1, 59 kDa
Short name=APP-BP1
Amyloid protein-binding protein 1

Gene names

Name:	Nae1
Synonyms:	Appbp1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation By similarity.
Enzyme regulation	Binding of TP53BP2 to the regulatory subunit NAE1 decreases neddylation activity By similarity.
Pathway	Protein modification; protein neddylation.
Subunit structure	Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2 By similarity.
Subcellular location	Cell membrane By similarity. Note: Colocalizes with APP in lipid rafts By similarity.
Sequence similarities	Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.

Ontologies

Keywords
Biological process	Apoptosis Cell cycle Ubl conjugation pathway
Cellular component	Cell membrane Membrane
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	NEDD8-activating enzyme E1 regulatory subunit		PRO_0000194953
Regions
	Region	331 – 344	14	Interaction with UBA3 By similarity
Sites
	Site	211	1	Interaction with UBA3 By similarity
Amino acid modifications
	Modified residue	6	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	127	1	V → F in AAH23680. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q8VBW6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 5CFCABFE5FA7AC62	FASTA	534	60,274
	10 20 30 40 50 60 MAQPGKILKE QKYDRQLRLW GDHGQEALES AHVCLINATA TGTEILKNLV LPGIGSFTII 70 80 90 100 110 120 DGNLVSGEDA GNNFFLQKSS IGKNRAQAAM EFLQELNSDV SGSFVEESPE NLLDNDPSFF 130 140 150 160 170 180 CRFTIVVATQ LLESTLLRLA DVLWNSQIPL LICRTYGLVG YMRIIIKEHP VIESHPDNAL 190 200 210 220 230 240 EDLRLDKPFP ELREHLQSYD LDHMEKKDHS HTPWIVIIAK YLAQWYNETN GRIPKSYKEK 250 260 270 280 290 300 EDFRDLIRQG ILKNENGAPE DEENFEEAIK NVNTALNTTQ IPSSIEDIFN DDRCINITKQ 310 320 330 340 350 360 TPTFWILARA LKEFVAKEGQ GNLPVRGTIP DMIADSNKYI KLQNVYREKA KKDAAAVGNH 370 380 390 400 410 420 VAKLLQSVGQ APESISEKEL KLLCSNSAFL RVVRCRSLAE EYGLDTVNKD EIISSMDNPD 430 440 450 460 470 480 NEIVLYLMLR AVDRFHKQHG RYPGVSNYQV EEDIGKLKSC LTGFLQEYGL SVMVKDDYVH 490 500 510 520 530 EFCRYGAAEP HTIAAFLGGA AAQEVIKIIT KQFVIFNNTY IYSGMSQTSA TFQL

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC019163 mRNA. Translation: AAH19163.1. BC021510 mRNA. Translation: AAH21510.1. BC023680 mRNA. Translation: AAH23680.1. BC023897 mRNA. Translation: AAH23897.1. BC027124 mRNA. Translation: AAH27124.1.
IPI	IPI00404981.
RefSeq	NP_659180.1. NM_144931.3.
UniGene	Mm.237670.
3D structure databases
ProteinModelPortal	Q8VBW6.
SMR	Q8VBW6. Positions 9-534.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8VBW6.
PTM databases
PhosphoSite	Q8VBW6.
Proteomic databases
PRIDE	Q8VBW6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034349; ENSMUSP00000034349; ENSMUSG00000031878.
GeneID	234664.
KEGG	mmu:234664.
Organism-specific databases
CTD	8883.
MGI	MGI:2384561. Nae1.
Phylogenomic databases
GeneTree	ENSGT00550000074901.
HOGENOM	HBG736236.
HOVERGEN	HBG079761.
InParanoid	Q8VBW6.
OMA	NGAPEDE.
OrthoDB	EOG46DM2F.
PhylomeDB	Q8VBW6.
Gene expression databases
ArrayExpress	Q8VBW6.
Bgee	Q8VBW6.
CleanEx	MM_NAE1.
Genevestigator	Q8VBW6.
GermOnline	ENSMUSG00000031878. Mus musculus.
Family and domain databases
InterPro	IPR009036. Molybdenum_cofac_synth_MoeB. IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 3 hits.
KO	K04532.
Pfam	PF00899. ThiF. 1 hit. [Graphical view]
SUPFAM	SSF69572. MoeB. 1 hit.
ProtoNet	Search...
Other
NextBio	382253.
SOURCE	Search...

Entry information

Entry name

ULA1_MOUSE

Accession

Primary (citable) accession number: Q8VBW6
Secondary accession number(s): Q8CFL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	March 1, 2002
Last modified:	December 14, 2011
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program