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Protein

NEDD8-activating enzyme E1 regulatory subunit

Gene

Nae1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation (By similarity).By similarity

Enzyme regulationi

Binding of TP53BP2 to the regulatory subunit NAE1 decreases neddylation activity.By similarity

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis, Cell cycle, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00885.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-activating enzyme E1 regulatory subunit
Alternative name(s):
Amyloid beta precursor protein-binding protein 1, 59 kDa
Short name:
APP-BP1
Amyloid protein-binding protein 1
Gene namesi
Name:Nae1
Synonyms:Appbp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2384561. Nae1.

Subcellular locationi

  • Cell membrane By similarity

  • Note: Colocalizes with APP in lipid rafts.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 534533NEDD8-activating enzyme E1 regulatory subunitPRO_0000194953Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei6 – 61N6-acetyllysineBy similarity
Modified residuei341 – 3411N6-acetyllysineCombined sources

Post-translational modificationi

Ubiquitinated by TRIP12, leading to its degradation by the proteasome.By similarity

Keywords - PTMi

Acetylation, Ubl conjugation

Proteomic databases

EPDiQ8VBW6.
MaxQBiQ8VBW6.
PaxDbiQ8VBW6.
PRIDEiQ8VBW6.

PTM databases

iPTMnetiQ8VBW6.
PhosphoSiteiQ8VBW6.

Expressioni

Gene expression databases

BgeeiQ8VBW6.
CleanExiMM_NAE1.
ExpressionAtlasiQ8VBW6. baseline and differential.
GenevisibleiQ8VBW6. MM.

Interactioni

Subunit structurei

Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei211 – 2111Interaction with UBA3By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ8VBW6. 1 interaction.
MINTiMINT-4088001.
STRINGi10090.ENSMUSP00000034349.

Structurei

3D structure databases

ProteinModelPortaliQ8VBW6.
SMRiQ8VBW6. Positions 9-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni331 – 34414Interaction with UBA3By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2016. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00550000074901.
HOGENOMiHOG000216537.
HOVERGENiHBG079761.
InParanoidiQ8VBW6.
KOiK04532.
OMAiCRKANIV.
PhylomeDBiQ8VBW6.
TreeFamiTF313972.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR030667. APP-BP1.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
PIRSFiPIRSF039099. APP-BP1. 1 hit.
SUPFAMiSSF69572. SSF69572. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VBW6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQPGKILKE QKYDRQLRLW GDHGQEALES AHVCLINATA TGTEILKNLV
60 70 80 90 100
LPGIGSFTII DGNLVSGEDA GNNFFLQKSS IGKNRAQAAM EFLQELNSDV
110 120 130 140 150
SGSFVEESPE NLLDNDPSFF CRFTIVVATQ LLESTLLRLA DVLWNSQIPL
160 170 180 190 200
LICRTYGLVG YMRIIIKEHP VIESHPDNAL EDLRLDKPFP ELREHLQSYD
210 220 230 240 250
LDHMEKKDHS HTPWIVIIAK YLAQWYNETN GRIPKSYKEK EDFRDLIRQG
260 270 280 290 300
ILKNENGAPE DEENFEEAIK NVNTALNTTQ IPSSIEDIFN DDRCINITKQ
310 320 330 340 350
TPTFWILARA LKEFVAKEGQ GNLPVRGTIP DMIADSNKYI KLQNVYREKA
360 370 380 390 400
KKDAAAVGNH VAKLLQSVGQ APESISEKEL KLLCSNSAFL RVVRCRSLAE
410 420 430 440 450
EYGLDTVNKD EIISSMDNPD NEIVLYLMLR AVDRFHKQHG RYPGVSNYQV
460 470 480 490 500
EEDIGKLKSC LTGFLQEYGL SVMVKDDYVH EFCRYGAAEP HTIAAFLGGA
510 520 530
AAQEVIKIIT KQFVIFNNTY IYSGMSQTSA TFQL
Length:534
Mass (Da):60,274
Last modified:March 1, 2002 - v1
Checksum:i5CFCABFE5FA7AC62
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti127 – 1271V → F in AAH23680 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019163 mRNA. Translation: AAH19163.1.
BC021510 mRNA. Translation: AAH21510.1.
BC023680 mRNA. Translation: AAH23680.1.
BC023897 mRNA. Translation: AAH23897.1.
BC027124 mRNA. Translation: AAH27124.1.
CCDSiCCDS40452.1.
RefSeqiNP_659180.1. NM_144931.3.
UniGeneiMm.237670.

Genome annotation databases

EnsembliENSMUST00000034349; ENSMUSP00000034349; ENSMUSG00000031878.
GeneIDi234664.
KEGGimmu:234664.
UCSCiuc009nas.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019163 mRNA. Translation: AAH19163.1.
BC021510 mRNA. Translation: AAH21510.1.
BC023680 mRNA. Translation: AAH23680.1.
BC023897 mRNA. Translation: AAH23897.1.
BC027124 mRNA. Translation: AAH27124.1.
CCDSiCCDS40452.1.
RefSeqiNP_659180.1. NM_144931.3.
UniGeneiMm.237670.

3D structure databases

ProteinModelPortaliQ8VBW6.
SMRiQ8VBW6. Positions 9-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VBW6. 1 interaction.
MINTiMINT-4088001.
STRINGi10090.ENSMUSP00000034349.

PTM databases

iPTMnetiQ8VBW6.
PhosphoSiteiQ8VBW6.

Proteomic databases

EPDiQ8VBW6.
MaxQBiQ8VBW6.
PaxDbiQ8VBW6.
PRIDEiQ8VBW6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034349; ENSMUSP00000034349; ENSMUSG00000031878.
GeneIDi234664.
KEGGimmu:234664.
UCSCiuc009nas.2. mouse.

Organism-specific databases

CTDi8883.
MGIiMGI:2384561. Nae1.

Phylogenomic databases

eggNOGiKOG2016. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00550000074901.
HOGENOMiHOG000216537.
HOVERGENiHBG079761.
InParanoidiQ8VBW6.
KOiK04532.
OMAiCRKANIV.
PhylomeDBiQ8VBW6.
TreeFamiTF313972.

Enzyme and pathway databases

UniPathwayiUPA00885.

Miscellaneous databases

PROiQ8VBW6.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VBW6.
CleanExiMM_NAE1.
ExpressionAtlasiQ8VBW6. baseline and differential.
GenevisibleiQ8VBW6. MM.

Family and domain databases

Gene3Di3.40.50.720. 3 hits.
InterProiIPR030667. APP-BP1.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
PIRSFiPIRSF039099. APP-BP1. 1 hit.
SUPFAMiSSF69572. SSF69572. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-341, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiULA1_MOUSE
AccessioniPrimary (citable) accession number: Q8VBW6
Secondary accession number(s): Q8CFL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2002
Last modified: June 8, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.