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Protein

HMG box transcription factor BBX

Gene

Bbx

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that is necessary for cell cycle progression from G1 to S phase.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi80 – 14869HMG boxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • bone development Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HMG box transcription factor BBX
Alternative name(s):
Bobby sox homolog
HMG box-containing protein 2
Gene namesi
Name:Bbx
Synonyms:Hbp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1917758. Bbx.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 907907HMG box transcription factor BBXPRO_0000232886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421PhosphoserineBy similarity
Modified residuei483 – 4831PhosphoserineBy similarity
Modified residuei701 – 7011PhosphoserineBy similarity
Modified residuei789 – 7891PhosphoserineBy similarity
Modified residuei811 – 8111PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8VBW5.
PRIDEiQ8VBW5.

PTM databases

PhosphoSiteiQ8VBW5.

Expressioni

Gene expression databases

BgeeiQ8VBW5.
CleanExiMM_BBX.
ExpressionAtlasiQ8VBW5. baseline and differential.
GenevisibleiQ8VBW5. MM.

Structurei

Secondary structure

1
907
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi86 – 10116Combined sources
Beta strandi103 – 1053Combined sources
Helixi109 – 11911Combined sources
Helixi123 – 14018Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WZ6NMR-A80-148[»]
ProteinModelPortaliQ8VBW5.
SMRiQ8VBW5. Positions 80-153.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VBW5.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi41 – 5010Poly-Glu
Compositional biasi444 – 54097Lys-richAdd
BLAST
Compositional biasi715 – 7184Poly-Lys

Sequence similaritiesi

Contains 1 HMG box DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG305473.
GeneTreeiENSGT00530000063757.
HOVERGENiHBG079777.
InParanoidiQ8VBW5.
OMAiFQFAEIS.
OrthoDBiEOG77DJ8X.
PhylomeDBiQ8VBW5.
TreeFamiTF106402.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
IPR019102. TF_HMG_box_BBX_DUF2028.
[Graphical view]
PfamiPF09667. DUF2028. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VBW5-1) [UniParc]FASTAAdd to basket

Also known as: BbxA

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKGSNRNKDH STEGEGDGKR PKRKCLQWHP LLAKKLLDFS EEEEEDEEEE
60 70 80 90 100
DIDKVQLLEA DGLEQDVAET EDDESPEQRA RRPMNAFLLF CKRHRSLVRQ
110 120 130 140 150
EHPRLDNRGA TKILADWWAV LDPKEKQKYT DMAKEYKDAF MKANPGYRWC
160 170 180 190 200
PTTNKPVKSP TPTVNPRKKL WAFPPDSSRD LPTPKKAKTE VPQLNFGMAD
210 220 230 240 250
PTQMGGLSML LLAGEHALGT PEASSGTCRP DISESPELRQ KSPLFQFAEI
260 270 280 290 300
SSRTSHPDAP SKQCQASALF QFAEISSSTS QLGGTEPVKR CGNSALFQLA
310 320 330 340 350
EMCLASEGVK MEDTKLIKSK ESDGGRIEEI EKGKEERGTE VEKTTETSFQ
360 370 380 390 400
KEAEFGKSAK GNVRESKDLR DIEQLQMDNV MAIKVEDPKE IRKEPEDDQK
410 420 430 440 450
YSHFPDFSYS ASSKIIISGV PSRKDHMCHP HGIMIIEDPT TLNKPEKIKK
460 470 480 490 500
KKKKNKLDRH GNDKSTPKKT CKKRQSSESD IESVMYTIEA VAKGDWGVDK
510 520 530 540 550
LGETPRKKVR PSSSGKGGIL DAKPPKKKVK SKEKKVSKEK CSDITKESRP
560 570 580 590 600
PDFLSISASK SVPGEVPEGI KAEPLTPTED ALPPSLPGQA KPEDSDCHRK
610 620 630 640 650
TETCGSRKSE RSCKGALYKT LVSEGMLTSL RANVDRGKRS SGKGNSSDHE
660 670 680 690 700
GCWSEESWTF NQSGTSGSKK FKKKLREDSF LGSAKLDEEF EKKFNSLPQY
710 720 730 740 750
SPITFDRKCV STPRKKKKTG NMSSESTKTS KGSGDKWSHK QFFLDAIHPT
760 770 780 790 800
EAIFSEDKST TEPAFKVKNA LSIPNTPEPT TMQEPLVGSQ KRKARKTKIT
810 820 830 840 850
HLVRTADGRV SPAGGTLDDK PKEQLQRSLP KVPGTYCGDN CSHSTVEEPR
860 870 880 890 900
SSTPDMPAVS AFFSLAALAE VAAMENVHRG QRSTPLTHDG QPKEMPQAPV

LISCADQ
Length:907
Mass (Da):100,783
Last modified:April 18, 2006 - v2
Checksum:i53E113D4B3DE4DAC
GO
Isoform 2 (identifier: Q8VBW5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     55-135: Missing.
     223-249: Missing.
     730-730: S → SKGPFQSQKKNLFHKIVSKYKHKKEKPNVPE

Show »
Length:829
Mass (Da):91,878
Checksum:i3685A4E65B4A64EF
GO
Isoform 3 (identifier: Q8VBW5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     732-751: Missing.

Show »
Length:887
Mass (Da):98,500
Checksum:iB1913D47BAE14B1C
GO
Isoform 4 (identifier: Q8VBW5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     733-750: SGDKWSHKQFFLDAIHPT → PFQSQKKNLFHKIVSKYK
     751-907: Missing.

Show »
Length:750
Mass (Da):83,986
Checksum:i5035FF87DBAEAF10
GO

Sequence cautioni

The sequence AAL68987.1 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAL68988.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti269 – 2691L → F in AAL58872 (Ref. 1) Curated
Sequence conflicti269 – 2691L → F in AAL58873 (Ref. 1) Curated
Sequence conflicti269 – 2691L → F in BAB30768 (PubMed:16141072).Curated
Sequence conflicti450 – 4501K → R in BAC34285 (PubMed:16141072).Curated
Sequence conflicti455 – 4551N → T in AAL58872 (Ref. 1) Curated
Sequence conflicti455 – 4551N → T in AAL58873 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti30 – 301P → L in strain: C3H/He. 1 Publication
Natural varianti281 – 2811Q → H in strain: C3H/He. 1 Publication

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei55 – 13581Missing in isoform 2. 1 PublicationVSP_018007Add
BLAST
Alternative sequencei223 – 24927Missing in isoform 2. 1 PublicationVSP_018008Add
BLAST
Alternative sequencei730 – 7301S → SKGPFQSQKKNLFHKIVSKY KHKKEKPNVPE in isoform 2. 1 PublicationVSP_018009
Alternative sequencei732 – 75120Missing in isoform 3. 1 PublicationVSP_018010Add
BLAST
Alternative sequencei733 – 75018SGDKW…AIHPT → PFQSQKKNLFHKIVSKYK in isoform 4. 1 PublicationVSP_018011Add
BLAST
Alternative sequencei751 – 907157Missing in isoform 4. 1 PublicationVSP_018012Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF454943 mRNA. Translation: AAL58872.1.
AF454944 mRNA. Translation: AAL58873.1.
AK017487 mRNA. Translation: BAB30768.1.
AK029532 mRNA. Translation: BAC26500.2.
AK029747 mRNA. Translation: BAC26596.1.
AK049516 mRNA. Translation: BAC33788.1.
AK050488 mRNA. Translation: BAC34285.1.
AK157813 mRNA. Translation: BAE34207.1.
CT571273, AC109627 Genomic DNA. Translation: CAX15928.1.
CT571273, AC109627 Genomic DNA. Translation: CAX15929.1.
CT571273, AC109627 Genomic DNA. Translation: CAX15930.1.
CT571273, AC109627 Genomic DNA. Translation: CAX15931.1.
BC057869 mRNA. Translation: AAH57869.1.
BC066821 mRNA. Translation: AAH66821.1.
AF276950 mRNA. Translation: AAL68986.1.
AF276951 mRNA. Translation: AAL68987.1. Sequence problems.
AF276952 mRNA. Translation: AAL68988.1. Different initiation.
CCDSiCCDS37354.1. [Q8VBW5-1]
RefSeqiNP_081720.2. NM_027444.3. [Q8VBW5-1]
XP_006522626.1. XM_006522563.2. [Q8VBW5-3]
UniGeneiMm.28940.

Genome annotation databases

EnsembliENSMUST00000066037; ENSMUSP00000066384; ENSMUSG00000022641. [Q8VBW5-2]
ENSMUST00000089399; ENSMUSP00000086821; ENSMUSG00000022641. [Q8VBW5-4]
ENSMUST00000089404; ENSMUSP00000086826; ENSMUSG00000022641. [Q8VBW5-3]
ENSMUST00000114488; ENSMUSP00000110132; ENSMUSG00000022641. [Q8VBW5-1]
ENSMUST00000138166; ENSMUSP00000119238; ENSMUSG00000022641. [Q8VBW5-1]
GeneIDi70508.
KEGGimmu:70508.
UCSCiuc007zkn.2. mouse. [Q8VBW5-1]
uc007zkp.2. mouse. [Q8VBW5-3]
uc007zkq.2. mouse. [Q8VBW5-2]
uc007zkr.2. mouse. [Q8VBW5-4]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF454943 mRNA. Translation: AAL58872.1.
AF454944 mRNA. Translation: AAL58873.1.
AK017487 mRNA. Translation: BAB30768.1.
AK029532 mRNA. Translation: BAC26500.2.
AK029747 mRNA. Translation: BAC26596.1.
AK049516 mRNA. Translation: BAC33788.1.
AK050488 mRNA. Translation: BAC34285.1.
AK157813 mRNA. Translation: BAE34207.1.
CT571273, AC109627 Genomic DNA. Translation: CAX15928.1.
CT571273, AC109627 Genomic DNA. Translation: CAX15929.1.
CT571273, AC109627 Genomic DNA. Translation: CAX15930.1.
CT571273, AC109627 Genomic DNA. Translation: CAX15931.1.
BC057869 mRNA. Translation: AAH57869.1.
BC066821 mRNA. Translation: AAH66821.1.
AF276950 mRNA. Translation: AAL68986.1.
AF276951 mRNA. Translation: AAL68987.1. Sequence problems.
AF276952 mRNA. Translation: AAL68988.1. Different initiation.
CCDSiCCDS37354.1. [Q8VBW5-1]
RefSeqiNP_081720.2. NM_027444.3. [Q8VBW5-1]
XP_006522626.1. XM_006522563.2. [Q8VBW5-3]
UniGeneiMm.28940.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WZ6NMR-A80-148[»]
ProteinModelPortaliQ8VBW5.
SMRiQ8VBW5. Positions 80-153.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8VBW5.

Proteomic databases

MaxQBiQ8VBW5.
PRIDEiQ8VBW5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000066037; ENSMUSP00000066384; ENSMUSG00000022641. [Q8VBW5-2]
ENSMUST00000089399; ENSMUSP00000086821; ENSMUSG00000022641. [Q8VBW5-4]
ENSMUST00000089404; ENSMUSP00000086826; ENSMUSG00000022641. [Q8VBW5-3]
ENSMUST00000114488; ENSMUSP00000110132; ENSMUSG00000022641. [Q8VBW5-1]
ENSMUST00000138166; ENSMUSP00000119238; ENSMUSG00000022641. [Q8VBW5-1]
GeneIDi70508.
KEGGimmu:70508.
UCSCiuc007zkn.2. mouse. [Q8VBW5-1]
uc007zkp.2. mouse. [Q8VBW5-3]
uc007zkq.2. mouse. [Q8VBW5-2]
uc007zkr.2. mouse. [Q8VBW5-4]

Organism-specific databases

CTDi56987.
MGIiMGI:1917758. Bbx.

Phylogenomic databases

eggNOGiNOG305473.
GeneTreeiENSGT00530000063757.
HOVERGENiHBG079777.
InParanoidiQ8VBW5.
OMAiFQFAEIS.
OrthoDBiEOG77DJ8X.
PhylomeDBiQ8VBW5.
TreeFamiTF106402.

Miscellaneous databases

EvolutionaryTraceiQ8VBW5.
NextBioi331763.
PROiQ8VBW5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VBW5.
CleanExiMM_BBX.
ExpressionAtlasiQ8VBW5. baseline and differential.
GenevisibleiQ8VBW5. MM.

Family and domain databases

Gene3Di1.10.30.10. 1 hit.
InterProiIPR009071. HMG_box_dom.
IPR019102. TF_HMG_box_BBX_DUF2028.
[Graphical view]
PfamiPF09667. DUF2028. 1 hit.
PF00505. HMG_box. 1 hit.
[Graphical view]
SMARTiSM00398. HMG. 1 hit.
[Graphical view]
SUPFAMiSSF47095. SSF47095. 1 hit.
PROSITEiPS50118. HMG_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "BBX is expressed in developing CNS and in neuronal tumours."
    Lee C.-J., Chan W.-I., Appleby V.J., Orme A.T., Scotting P.J.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
    Strain: C57BL/6J.
    Tissue: Embryo, Pancreas and Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS LEU-30 AND HIS-281.
    Strain: C3H/He and NMRI.
    Tissue: Mammary gland, Mammary tumor and Mesenchymal stem cell.
  5. "HBP2: a new mammalian protein that complements the fission yeast MBF transcription complex."
    Sanchez-Diaz A., Blanco M.A., Jones N., Moreno S.
    Curr. Genet. 40:110-118(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-454, FUNCTION.
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  6. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Solution structure of the HMG-box domain of murine bobby sox homolog."
    RIKEN structural genomics initiative (RSGI)
    Submitted (AUG-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 80-148.

Entry informationi

Entry nameiBBX_MOUSE
AccessioniPrimary (citable) accession number: Q8VBW5
Secondary accession number(s): B8JK46
, B8JK47, B8JK48, B8JK49, Q3TZK1, Q6NXY8, Q6PEU3, Q8BQJ7, Q8C7E0, Q8CDQ0, Q8CDV1, Q8VI48, Q8VI49, Q8VI50, Q9CS94
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: April 18, 2006
Last modified: June 24, 2015
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.