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Q8VBW1 (SC6A8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium- and chloride-dependent creatine transporter 1

Short name=CT1
Short name=Creatine transporter 1
Alternative name(s):
Solute carrier family 6 member 8
Gene names
Name:Slc6a8
Synonyms:Crt
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length640 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for the uptake of creatine. Plays an important role in supplying creatine to the brain via the blood-brain barrier.

Subcellular location

Membrane; Multi-pass membrane protein By similarity.

Sequence similarities

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A8 subfamily. [View classification]

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VBW1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VBW1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     465-469: Missing.
Isoform 3 (identifier: Q8VBW1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     462-469: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 640640Sodium- and chloride-dependent creatine transporter 1
PRO_0000214775

Regions

Topological domain1 – 6060Cytoplasmic Potential
Transmembrane61 – 8121Helical; Potential
Topological domain82 – 876Extracellular Potential
Transmembrane88 – 10821Helical; Potential
Topological domain109 – 13830Cytoplasmic Potential
Transmembrane139 – 15921Helical; Potential
Topological domain160 – 23071Extracellular Potential
Transmembrane231 – 25121Helical; Potential
Topological domain252 – 26918Cytoplasmic Potential
Transmembrane270 – 29021Helical; Potential
Topological domain291 – 30414Extracellular Potential
Transmembrane305 – 32521Helical; Potential
Topological domain326 – 34116Cytoplasmic Potential
Transmembrane342 – 36221Helical; Potential
Topological domain363 – 39432Extracellular Potential
Transmembrane395 – 41521Helical; Potential
Topological domain416 – 44429Cytoplasmic Potential
Transmembrane445 – 47026Helical; Potential
Topological domain471 – 48414Extracellular Potential
Transmembrane485 – 50521Helical; Potential
Topological domain506 – 52520Cytoplasmic Potential
Transmembrane526 – 54621Helical; Potential
Topological domain547 – 56519Extracellular Potential
Transmembrane566 – 58621Helical; Potential
Topological domain587 – 64054Cytoplasmic Potential

Amino acid modifications

Glycosylation1921N-linked (GlcNAc...) Potential
Glycosylation1971N-linked (GlcNAc...) Potential
Glycosylation5531N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence462 – 4698Missing in isoform 3.
VSP_012132
Alternative sequence465 – 4695Missing in isoform 2.
VSP_012133

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 67D3AAAC591EEA0B

FASTA64070,999
        10         20         30         40         50         60 
MAKKSAENGI YSVSGDEKKG PLIVSGPDGA PAKGDGPAGL GAPGGRLAVP PRETWTRQMD 

        70         80         90        100        110        120 
FIMSCVGFAV GLGNVWRFPY LCYKNGGGVF LIPYVLIALV GGIPIFFLEI SLGQFMKAGS 

       130        140        150        160        170        180 
INVWNICPLF KGLGYASMVI VFYCNTYYIM VLAWGFYYLV KSFTTTLPWA TCGHTWNTPD 

       190        200        210        220        230        240 
CVEIFRHEDC ANASLANLTC DQLADRRSPV IEFWENKVLR LSTGLEVPGA LNWEVTLCLL 

       250        260        270        280        290        300 
ACWVLVYFCV WKGVKSTGKI VYFTATFPYV VLVVLLVRGV LLPGALDGII YYLKPDWSKL 

       310        320        330        340        350        360 
GSPQVWIDAG TQIFFSYAIG LGALTALGSY NRFNNNCYKD AIILALINSG TSFFAGFVVF 

       370        380        390        400        410        420 
SILGFMATEQ GVHISKVAES GPGLAFIAYP RAVTLMPVAP LWAALFFFML LLLGLDSQFV 

       430        440        450        460        470        480 
GVEGFITGLL DLLPASYYFR FQREISVALC CALCFVIDLS MVTDVSGGKG GMYVFQLFDY 

       490        500        510        520        530        540 
YSASGTTLLW QAFWECVVVA WVYGADRFMD DIACMIGYRP CPWMKWCWSF FTPLVCMGIF 

       550        560        570        580        590        600 
IFNIVYYEPL VYNNTYVYPW WGEAMGWAFA LSSMLCVPLH LLGCLLRAKG TMAERWQHLT 

       610        620        630        640 
QPIWGLHHLE YRAQDADVRG LTTLTPVSES SKVVVVESVM 

« Hide

Isoform 2 [UniParc].

Checksum: FE76B1A95D2F2149
Show »

FASTA63570,571
Isoform 3 [UniParc].

Checksum: 7369D703B507FF24
Show »

FASTA63270,255

References

« Hide 'large scale' references
[1]"The blood-brain barrier creatine transporter is a major pathway for supplying creatine to the brain."
Ohtsuki S., Tachikawa M., Takanaga H., Shimizu H., Watanabe M., Hosoya K., Terasaki T.
J. Cereb. Blood Flow Metab. 22:1327-1335(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]"Cloning and expression of mouse creatine transporter gene."
Liu Q.-R., Li Q.-F.
Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 58-640 (ISOFORMS 2 AND 3).
Strain: FVB/N.
Tissue: Embryo and Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB077327 mRNA. Translation: BAC11857.1.
AF459435 mRNA. Translation: AAL66354.1.
AF459436 Genomic DNA. Translation: AAL66355.1.
AL805924 Genomic DNA. Translation: CAM21057.1.
BC024444 mRNA. Translation: AAH24444.1.
BC049801 mRNA. Translation: AAH49801.1.
RefSeqNP_001136281.1. NM_001142809.1.
NP_001136282.1. NM_001142810.1.
NP_598748.1. NM_133987.2.
UniGeneMm.274553.

3D structure databases

ProteinModelPortalQ8VBW1.
SMRQ8VBW1. Positions 50-603.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8VBW1.

Proteomic databases

PRIDEQ8VBW1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033752; ENSMUSP00000033752; ENSMUSG00000019558. [Q8VBW1-1]
ENSMUST00000114465; ENSMUSP00000110109; ENSMUSG00000019558. [Q8VBW1-2]
ENSMUST00000114467; ENSMUSP00000110111; ENSMUSG00000019558. [Q8VBW1-2]
GeneID102857.
KEGGmmu:102857.
UCSCuc009tmf.2. mouse. [Q8VBW1-1]
uc009tmg.2. mouse. [Q8VBW1-3]

Organism-specific databases

CTD6535.
MGIMGI:2147834. Slc6a8.

Phylogenomic databases

eggNOGCOG0733.
GeneTreeENSGT00730000110773.
HOGENOMHOG000116406.
HOVERGENHBG071421.
InParanoidQ8VBW1.
KOK05039.
OMAIVFYCNT.
OrthoDBEOG793B71.
PhylomeDBQ8VBW1.
TreeFamTF343812.

Gene expression databases

ArrayExpressQ8VBW1.
BgeeQ8VBW1.
GenevestigatorQ8VBW1.

Family and domain databases

InterProIPR000175. Na/ntran_symport.
IPR002984. Na/ntran_symport_creatine.
[Graphical view]
PANTHERPTHR11616. PTHR11616. 1 hit.
PfamPF00209. SNF. 1 hit.
[Graphical view]
PRINTSPR01199. CRTTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC6A8. mouse.
NextBio355683.
PROQ8VBW1.
SOURCESearch...

Entry information

Entry nameSC6A8_MOUSE
AccessionPrimary (citable) accession number: Q8VBW1
Secondary accession number(s): A2ALM4 expand/collapse secondary AC list , Q80YC9, Q8K4R3, Q8R1L0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot