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Protein

Sodium- and chloride-dependent creatine transporter 1

Gene

Slc6a8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the uptake of creatine. Plays an important role in supplying creatine to the brain via the blood-brain barrier.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_307447. Creatine metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium- and chloride-dependent creatine transporter 1
Short name:
CT1
Short name:
Creatine transporter 1
Alternative name(s):
Solute carrier family 6 member 8
Gene namesi
Name:Slc6a8
Synonyms:Crt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:2147834. Slc6a8.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6060CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei61 – 8121HelicalSequence AnalysisAdd
BLAST
Topological domaini82 – 876ExtracellularSequence Analysis
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Topological domaini109 – 13830CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei139 – 15921HelicalSequence AnalysisAdd
BLAST
Topological domaini160 – 23071ExtracellularSequence AnalysisAdd
BLAST
Transmembranei231 – 25121HelicalSequence AnalysisAdd
BLAST
Topological domaini252 – 26918CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei270 – 29021HelicalSequence AnalysisAdd
BLAST
Topological domaini291 – 30414ExtracellularSequence AnalysisAdd
BLAST
Transmembranei305 – 32521HelicalSequence AnalysisAdd
BLAST
Topological domaini326 – 34116CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei342 – 36221HelicalSequence AnalysisAdd
BLAST
Topological domaini363 – 39432ExtracellularSequence AnalysisAdd
BLAST
Transmembranei395 – 41521HelicalSequence AnalysisAdd
BLAST
Topological domaini416 – 44429CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei445 – 47026HelicalSequence AnalysisAdd
BLAST
Topological domaini471 – 48414ExtracellularSequence AnalysisAdd
BLAST
Transmembranei485 – 50521HelicalSequence AnalysisAdd
BLAST
Topological domaini506 – 52520CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei526 – 54621HelicalSequence AnalysisAdd
BLAST
Topological domaini547 – 56519ExtracellularSequence AnalysisAdd
BLAST
Transmembranei566 – 58621HelicalSequence AnalysisAdd
BLAST
Topological domaini587 – 64054CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 640640Sodium- and chloride-dependent creatine transporter 1PRO_0000214775Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi192 – 1921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi553 – 5531N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ8VBW1.
PRIDEiQ8VBW1.

PTM databases

PhosphoSiteiQ8VBW1.

Expressioni

Gene expression databases

BgeeiQ8VBW1.
ExpressionAtlasiQ8VBW1. baseline and differential.
GenevisibleiQ8VBW1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033752.

Structurei

3D structure databases

ProteinModelPortaliQ8VBW1.
SMRiQ8VBW1. Positions 52-512.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ8VBW1.
KOiK05039.
OMAiSLMPVAP.
OrthoDBiEOG793B71.
PhylomeDBiQ8VBW1.
TreeFamiTF343812.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002984. Na/ntran_symport_creatine.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01199. CRTTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VBW1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKKSAENGI YSVSGDEKKG PLIVSGPDGA PAKGDGPAGL GAPGGRLAVP
60 70 80 90 100
PRETWTRQMD FIMSCVGFAV GLGNVWRFPY LCYKNGGGVF LIPYVLIALV
110 120 130 140 150
GGIPIFFLEI SLGQFMKAGS INVWNICPLF KGLGYASMVI VFYCNTYYIM
160 170 180 190 200
VLAWGFYYLV KSFTTTLPWA TCGHTWNTPD CVEIFRHEDC ANASLANLTC
210 220 230 240 250
DQLADRRSPV IEFWENKVLR LSTGLEVPGA LNWEVTLCLL ACWVLVYFCV
260 270 280 290 300
WKGVKSTGKI VYFTATFPYV VLVVLLVRGV LLPGALDGII YYLKPDWSKL
310 320 330 340 350
GSPQVWIDAG TQIFFSYAIG LGALTALGSY NRFNNNCYKD AIILALINSG
360 370 380 390 400
TSFFAGFVVF SILGFMATEQ GVHISKVAES GPGLAFIAYP RAVTLMPVAP
410 420 430 440 450
LWAALFFFML LLLGLDSQFV GVEGFITGLL DLLPASYYFR FQREISVALC
460 470 480 490 500
CALCFVIDLS MVTDVSGGKG GMYVFQLFDY YSASGTTLLW QAFWECVVVA
510 520 530 540 550
WVYGADRFMD DIACMIGYRP CPWMKWCWSF FTPLVCMGIF IFNIVYYEPL
560 570 580 590 600
VYNNTYVYPW WGEAMGWAFA LSSMLCVPLH LLGCLLRAKG TMAERWQHLT
610 620 630 640
QPIWGLHHLE YRAQDADVRG LTTLTPVSES SKVVVVESVM
Length:640
Mass (Da):70,999
Last modified:March 1, 2002 - v1
Checksum:i67D3AAAC591EEA0B
GO
Isoform 2 (identifier: Q8VBW1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     465-469: Missing.

Show »
Length:635
Mass (Da):70,571
Checksum:iFE76B1A95D2F2149
GO
Isoform 3 (identifier: Q8VBW1-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     462-469: Missing.

Note: No experimental confirmation available.
Show »
Length:632
Mass (Da):70,255
Checksum:i7369D703B507FF24
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei462 – 4698Missing in isoform 3. 1 PublicationVSP_012132
Alternative sequencei465 – 4695Missing in isoform 2. 2 PublicationsVSP_012133

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077327 mRNA. Translation: BAC11857.1.
AF459435 mRNA. Translation: AAL66354.1.
AF459436 Genomic DNA. Translation: AAL66355.1.
AL805924 Genomic DNA. Translation: CAM21057.1.
BC024444 mRNA. Translation: AAH24444.1.
BC049801 mRNA. Translation: AAH49801.1.
CCDSiCCDS30208.1. [Q8VBW1-1]
CCDS53098.1. [Q8VBW1-2]
RefSeqiNP_001136281.1. NM_001142809.1. [Q8VBW1-2]
NP_001136282.1. NM_001142810.1. [Q8VBW1-3]
NP_598748.1. NM_133987.2. [Q8VBW1-1]
UniGeneiMm.274553.

Genome annotation databases

EnsembliENSMUST00000033752; ENSMUSP00000033752; ENSMUSG00000019558. [Q8VBW1-1]
GeneIDi102857.
KEGGimmu:102857.
UCSCiuc009tmf.2. mouse. [Q8VBW1-1]
uc009tmg.2. mouse. [Q8VBW1-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB077327 mRNA. Translation: BAC11857.1.
AF459435 mRNA. Translation: AAL66354.1.
AF459436 Genomic DNA. Translation: AAL66355.1.
AL805924 Genomic DNA. Translation: CAM21057.1.
BC024444 mRNA. Translation: AAH24444.1.
BC049801 mRNA. Translation: AAH49801.1.
CCDSiCCDS30208.1. [Q8VBW1-1]
CCDS53098.1. [Q8VBW1-2]
RefSeqiNP_001136281.1. NM_001142809.1. [Q8VBW1-2]
NP_001136282.1. NM_001142810.1. [Q8VBW1-3]
NP_598748.1. NM_133987.2. [Q8VBW1-1]
UniGeneiMm.274553.

3D structure databases

ProteinModelPortaliQ8VBW1.
SMRiQ8VBW1. Positions 52-512.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033752.

PTM databases

PhosphoSiteiQ8VBW1.

Proteomic databases

MaxQBiQ8VBW1.
PRIDEiQ8VBW1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033752; ENSMUSP00000033752; ENSMUSG00000019558. [Q8VBW1-1]
GeneIDi102857.
KEGGimmu:102857.
UCSCiuc009tmf.2. mouse. [Q8VBW1-1]
uc009tmg.2. mouse. [Q8VBW1-3]

Organism-specific databases

CTDi6535.
MGIiMGI:2147834. Slc6a8.

Phylogenomic databases

eggNOGiCOG0733.
GeneTreeiENSGT00760000118857.
HOGENOMiHOG000116406.
HOVERGENiHBG071421.
InParanoidiQ8VBW1.
KOiK05039.
OMAiSLMPVAP.
OrthoDBiEOG793B71.
PhylomeDBiQ8VBW1.
TreeFamiTF343812.

Enzyme and pathway databases

ReactomeiREACT_307447. Creatine metabolism.

Miscellaneous databases

ChiTaRSiSlc6a8. mouse.
NextBioi355683.
PROiQ8VBW1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VBW1.
ExpressionAtlasiQ8VBW1. baseline and differential.
GenevisibleiQ8VBW1. MM.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
IPR002984. Na/ntran_symport_creatine.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 1 hit.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR01199. CRTTRANSPORT.
PR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The blood-brain barrier creatine transporter is a major pathway for supplying creatine to the brain."
    Ohtsuki S., Tachikawa M., Takanaga H., Shimizu H., Watanabe M., Hosoya K., Terasaki T.
    J. Cereb. Blood Flow Metab. 22:1327-1335(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  2. "Cloning and expression of mouse creatine transporter gene."
    Liu Q.-R., Li Q.-F.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE (ISOFORM 1).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 58-640 (ISOFORMS 2 AND 3).
    Strain: FVB/N.
    Tissue: Embryo and Kidney.

Entry informationi

Entry nameiSC6A8_MOUSE
AccessioniPrimary (citable) accession number: Q8VBW1
Secondary accession number(s): A2ALM4
, Q80YC9, Q8K4R3, Q8R1L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.