Q8VBU2 (NDRG2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NDRG2

Alternative name(s):
Antidepressant-related protein ADRG123
N-myc downstream-regulated gene 2 protein
NDRG1-related protein

Gene names

Name:

Ndrg2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor By similarity. May be involved in dendritic cell and neuron differentiation. Ref.5
Subcellular location	Cytoplasm By similarity. Cytoplasm › perinuclear region By similarity. Cell projection › growth cone. Note: In neurons, seems to concentrate at axonal growth cone.
Tissue specificity	Broadly expressed, with highest levels in heart, liver, skeletal muscle and aorta. Ref.1
Induction	Up-regulated by aldosterone in kidney and colon, but not in heart. Down-regulated in cortex by antidepressant treatments. Ref.1 Ref.2
Sequence similarities	Belongs to the NDRG family.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Wnt signaling pathway
Cellular component	Cell projection Cytoplasm
Coding sequence diversity	Alternative splicing
Disease	Tumor suppressor
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Wnt receptor signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara negative regulation of cytokine production Inferred from electronic annotation. Source: Compara negative regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara nervous system development Inferred from electronic annotation. Source: UniProtKB-KW regulation of platelet-derived growth factor production Inferred from electronic annotation. Source: Compara regulation of vascular endothelial growth factor production Inferred from electronic annotation. Source: Compara signal transduction Inferred from direct assay Ref.1. Source: RGD
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara centrosome Inferred from electronic annotation. Source: Compara growth cone Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: Compara perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VBU2-1)

Also known as: Ndrg2L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VBU2-2)

Also known as: Ndrg2S;

The sequence of this isoform differs from the canonical sequence as follows:
     26-39: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Protein NDRG2		PRO_0000238966
Amino acid modifications
	Modified residue	326	1	Phosphoserine By similarity
	Modified residue	328	1	Phosphoserine By similarity
	Modified residue	330	1	Phosphothreonine By similarity
	Modified residue	332	1	Phosphoserine Ref.4 Ref.6
	Modified residue	334	1	Phosphothreonine By similarity
	Modified residue	335	1	Phosphoserine By similarity
	Modified residue	338	1	Phosphoserine Ref.6
	Modified residue	342	1	Phosphoserine By similarity
	Modified residue	344	1	Phosphoserine By similarity
	Modified residue	346	1	Phosphoserine By similarity
	Modified residue	348	1	Phosphothreonine By similarity
	Modified residue	350	1	Phosphoserine By similarity
	Modified residue	352	1	Phosphoserine By similarity
	Modified residue	353	1	Phosphoserine By similarity
	Modified residue	355	1	Phosphoserine By similarity
	Modified residue	357	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	26 – 39	14	Missing in isoform 2.		VSP_019032
Experimental info
	Sequence conflict	121	1	Q → L in AAL73186. Ref.2
	Sequence conflict	121	1	Q → L in AAL73187. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Ndrg2L) [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C049A1AAF37D115A	FASTA	371	40,779
	10 20 30 40 50 60 MAELQEVQIT EEKPLLPGQT PEAAKEAELA ARILLDQGQT HSVETPYGSV TFTVYGTPKP 70 80 90 100 110 120 KRPAIFTYHD VGLNYKSCFQ PLFQFGDMQE IIQNFVRVHV DAPGMEEGAP VFPLGYQYPS 130 140 150 160 170 180 QDQLADMIPC ILQYLNFSTI IGVGVGAGAY ILSRYALNHP DTVEGLVLIN IDPNAKGWMD 190 200 210 220 230 240 WAAHKLTGLT SSIPEMILGH LFSQEELSGN SELIQKYRSL ITHAPNLENI ELYWNSYNNR 250 260 270 280 290 300 RDLNFERGGE MTLKCPVMLV VGDQAPHEDA VVECNSKLDP TQTSFLKMAD SGGQPQLTQP 310 320 330 340 350 360 GKLTEAFKYF VQGMGYMASS CMTRLSRSRT ASLTSAASID GSRSRSRTLS QSSESGTLPS 370 GPPGHTMEVS C
	Isoform 2 (Ndrg2S) [UniParc]. Checksum: 66792DC7A9DD9485	FASTA	357	39,270
	10 20 30 40 50 60 MAELQEVQIT EEKPLLPGQT PEAAKTHSVE TPYGSVTFTV YGTPKPKRPA IFTYHDVGLN 70 80 90 100 110 120 YKSCFQPLFQ FGDMQEIIQN FVRVHVDAPG MEEGAPVFPL GYQYPSQDQL ADMIPCILQY 130 140 150 160 170 180 LNFSTIIGVG VGAGAYILSR YALNHPDTVE GLVLINIDPN AKGWMDWAAH KLTGLTSSIP 190 200 210 220 230 240 EMILGHLFSQ EELSGNSELI QKYRSLITHA PNLENIELYW NSYNNRRDLN FERGGEMTLK 250 260 270 280 290 300 CPVMLVVGDQ APHEDAVVEC NSKLDPTQTS FLKMADSGGQ PQLTQPGKLT EAFKYFVQGM 310 320 330 340 350 GYMASSCMTR LSRSRTASLT SAASIDGSRS RSRTLSQSSE SGTLPSGPPG HTMEVSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of rat NDRG2 (N-Myc downstream regulated gene 2), a novel early mineralocorticoid-specific induced gene." Boulkroun S., Fay M., Zennaro M.-C., Escoubet B., Jaisser F., Blot-Chabaud M., Farman N., Courtois-Coutry N. J. Biol. Chem. 277:31506-31515(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY, INDUCTION. Strain: Sprague-Dawley. Tissue: Heart.
[2]	"Expression of Ndrg2 in the rat frontal cortex after antidepressant and electroconvulsive treatment." Takahashi K., Yamada M., Ohata H., Momose K., Higuchi T., Honda K., Yamada M. Int. J. Neuropsychopharmacol. 8:381-389(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INDUCTION.
[3]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 62-76 AND 155-176, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.
[4]	"Akt mediates insulin-stimulated phosphorylation of Ndrg2: evidence for cross-talk with protein kinase C theta." Burchfield J.G., Lennard A.J., Narasimhan S., Hughes W.E., Wasinger V.C., Corthals G.L., Okuda T., Kondoh H., Biden T.J., Schmitz-Peiffer C. J. Biol. Chem. 279:18623-18632(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY (ISOFORM 1), PHOSPHORYLATION AT SER-332.
[5]	"Ndrg2 promotes neurite outgrowth of NGF-differentiated PC12 cells." Takahashi K., Yamada M., Ohata H., Honda K., Yamada M. Neurosci. Lett. 388:157-162(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[6]	"Phosphoproteomic analysis of rat liver by high capacity IMAC and LC-MS/MS." Moser K., White F.M. J. Proteome Res. 5:98-104(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-332 AND SER-338, MASS SPECTROMETRY. Strain: Fischer. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ426424 mRNA. Translation: CAD19997.1. AJ426425 mRNA. Translation: CAD19998.1. AJ426426 mRNA. Translation: CAD19999.1. AJ426427 mRNA. Translation: CAD20000.1. AF334105 mRNA. Translation: AAL73186.1. AF334106 mRNA. Translation: AAL73187.1.
IPI	IPI00326606. IPI00382069.
RefSeq	NP_001257791.1. NM_001270862.1. NP_001257792.1. NM_001270863.1. NP_001257793.1. NM_001270864.1. NP_598267.2. NM_133583.2.
UniGene	Rn.3407.
3D structure databases
ProteinModelPortal	Q8VBU2.
SMR	Q8VBU2. Positions 39-313.
ModBase	Search...
PTM databases
PhosphoSite	Q8VBU2.
Proteomic databases
PaxDb	Q8VBU2.
PRIDE	Q8VBU2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000040540; ENSRNOP00000039619; ENSRNOG00000010389. ENSRNOT00000042055; ENSRNOP00000042949; ENSRNOG00000010389.
GeneID	171114.
KEGG	rno:171114.
UCSC	RGD:621874. rat.
Organism-specific databases
CTD	57447.
RGD	621874. Ndrg2.
Phylogenomic databases
eggNOG	NOG310435.
GeneTree	ENSGT00390000001874.
HOGENOM	HOG000230891.
HOVERGEN	HBG052591.
InParanoid	Q8VBU2.
OrthoDB	EOG4FBHTF.
Gene expression databases
Genevestigator	Q8VBU2.
GermOnline	ENSRNOG00000010389. Rattus norvegicus.
Family and domain databases
InterPro	IPR004142. Ndr. [Graphical view]
PANTHER	PTHR11034. PTHR11034. 1 hit.
Pfam	PF03096. Ndr. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	621832.

Entry information

Entry name

NDRG2_RAT

Accession

Primary (citable) accession number: Q8VBU2
Secondary accession number(s): Q8VBW2, Q8VI00, Q8VI01

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program