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Protein

Tether containing UBX domain for GLUT4

Gene

Aspscr1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Enhances VCP methylation catalyzed by VCPKMT (By similarity). Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.By similarity1 Publication

GO - Biological processi

  • glucose homeostasis Source: MGI
  • intracellular protein transport Source: MGI
  • regulation of glucose import Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Tether containing UBX domain for GLUT4
Alternative name(s):
Alveolar soft part sarcoma chromosomal region candidate gene 1 protein homolog
Gene namesi
Name:Aspscr1
Synonyms:Tug
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1916188. Aspscr1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic side of plasma membrane Source: MGI
  • cytoplasmic vesicle membrane Source: Reactome
  • cytosol Source: MGI
  • endomembrane system Source: UniProtKB-SubCell
  • endoplasmic reticulum-Golgi intermediate compartment membrane Source: UniProtKB-SubCell
  • extrinsic component of membrane Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • nucleoplasm Source: MGI
  • perinuclear region of cytoplasm Source: MGI
  • plasma membrane Source: MGI
  • vesicle membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 550549Tether containing UBX domain for GLUT4PRO_0000249886Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei193 – 1931PhosphoserineCombined sources
Modified residuei496 – 4961PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8VBT9.
MaxQBiQ8VBT9.
PaxDbiQ8VBT9.
PRIDEiQ8VBT9.

2D gel databases

REPRODUCTION-2DPAGEQ8VBT9.

PTM databases

iPTMnetiQ8VBT9.
PhosphoSiteiQ8VBT9.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

BgeeiQ8VBT9.
ExpressionAtlasiQ8VBT9. baseline and differential.
GenevisibleiQ8VBT9. MM.

Interactioni

Subunit structurei

Interacts with VCP. Interacts with VCPKMT (By similarity). Interacts with GLUT4.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026135.

Structurei

Secondary structure

1
550
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 154Combined sources
Beta strandi21 – 244Combined sources
Helixi32 – 4211Combined sources
Helixi47 – 493Combined sources
Beta strandi51 – 544Combined sources
Beta strandi57 – 626Combined sources
Helixi65 – 684Combined sources
Beta strandi75 – 795Combined sources
Beta strandi81 – 833Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AL3NMR-A1-90[»]
ProteinModelPortaliQ8VBT9.
SMRiQ8VBT9. Positions 10-85.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VBT9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini382 – 45877UBXPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni313 – 37664Interaction with GLUT4Add
BLAST

Sequence similaritiesi

Contains 1 UBX domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2699. Eukaryota.
ENOG410XQQK. LUCA.
GeneTreeiENSGT00840000129867.
HOGENOMiHOG000231241.
HOVERGENiHBG066860.
InParanoidiQ8VBT9.
KOiK15627.
OMAiMIKNLKR.
OrthoDBiEOG7S7SF9.
PhylomeDBiQ8VBT9.
TreeFamiTF320363.

Family and domain databases

InterProiIPR021569. TUG-UBL1.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF11470. TUG-UBL1. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 2 hits.
PROSITEiPS50033. UBX. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VBT9-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAPAGGGGS AVSVLAPNGR RHTVKVTPST VLLQVLEDTC RRQDFNPSEY
60 70 80 90 100
DLKFQRTVLD LSLQWRFANL PNNAKLEMVP VSRSREGPEN IVRIAFQLDD
110 120 130 140 150
GSRLQDAFCS RQTLWELLSH FAQTRERLQQ LGEKTPVCVY MRNEVTGRAA
160 170 180 190 200
LQNTTLQSLG LTGGSATIRF VIKQCDTAGK QESIAVRSKA PGSPVSSLSA
210 220 230 240 250
DQASSSTLLP LNSGEFSRGD LNHEGDANTS GTGLEGGPKP TDAQTKQSTS
260 270 280 290 300
EPASAPFVPF SGGGQRLGGP SASLRPLTSP SANSSKSFSG PGGPSKPKKP
310 320 330 340 350
KPGEEPQQEP EPPVDRDPVV YHPDLEDLLQ PWPAEVPDEF FEVTVDDVRR
360 370 380 390 400
RLAQLKSERK RLEEAPLVTK AFREAQMKEK LERYPKVALR VLFPDRYILQ
410 420 430 440 450
GFFRPSETVG DLRDFVRSHL GNPELSFYLF IAPPKMVLDD HTLTLFQANL
460 470 480 490 500
FPAALVHFGA EEPTGLYLEP GLLEHTVSPS TADVLVARCM SRASGSPPLL
510 520 530 540 550
PAPDPVSLES EPIAEDGALG PPEPIQGTAQ PVKRSLGKVP KWLKLPASKR
Length:550
Mass (Da):59,796
Last modified:March 1, 2002 - v1
Checksum:iCC2EB6F7BD43E113
GO
Isoform 2 (identifier: Q8VBT9-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.

Show »
Length:473
Mass (Da):51,346
Checksum:i12A84C6FDA12F24A
GO
Isoform 3 (identifier: Q8VBT9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-77: Missing.
     410-430: Missing.

Show »
Length:452
Mass (Da):48,881
Checksum:iBBD4C0CA283C4436
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7777Missing in isoform 2 and isoform 3. 2 PublicationsVSP_020579Add
BLAST
Alternative sequencei410 – 43021Missing in isoform 3. 1 PublicationVSP_020580Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY349132 mRNA. Translation: AAR01613.1.
AY349133 mRNA. Translation: AAR01614.1.
AK004509 mRNA. Translation: BAB23341.1.
AK146061 mRNA. Translation: BAE26870.1.
AL663030 Genomic DNA. Translation: CAM27105.1.
AL663030 Genomic DNA. Translation: CAM27114.1.
BC019177 mRNA. Translation: AAH19177.1.
BC022115 mRNA. Translation: AAH22115.1.
BC031153 mRNA. Translation: AAH31153.1.
CCDSiCCDS25751.1. [Q8VBT9-1]
CCDS25752.1. [Q8VBT9-2]
CCDS49008.1. [Q8VBT9-3]
RefSeqiNP_001157696.1. NM_001164224.1. [Q8VBT9-3]
NP_081153.1. NM_026877.2. [Q8VBT9-1]
NP_937866.1. NM_198223.2. [Q8VBT9-2]
XP_006534149.1. XM_006534086.2. [Q8VBT9-2]
XP_006534150.1. XM_006534087.2. [Q8VBT9-2]
UniGeneiMm.294020.

Genome annotation databases

EnsembliENSMUST00000026135; ENSMUSP00000026135; ENSMUSG00000025142. [Q8VBT9-1]
ENSMUST00000103016; ENSMUSP00000099305; ENSMUSG00000025142. [Q8VBT9-2]
ENSMUST00000106158; ENSMUSP00000101764; ENSMUSG00000025142. [Q8VBT9-3]
ENSMUST00000106159; ENSMUSP00000101765; ENSMUSG00000025142. [Q8VBT9-2]
ENSMUST00000106160; ENSMUSP00000101766; ENSMUSG00000025142. [Q8VBT9-3]
GeneIDi68938.
KEGGimmu:68938.
UCSCiuc007mua.2. mouse. [Q8VBT9-1]
uc007mub.1. mouse. [Q8VBT9-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY349132 mRNA. Translation: AAR01613.1.
AY349133 mRNA. Translation: AAR01614.1.
AK004509 mRNA. Translation: BAB23341.1.
AK146061 mRNA. Translation: BAE26870.1.
AL663030 Genomic DNA. Translation: CAM27105.1.
AL663030 Genomic DNA. Translation: CAM27114.1.
BC019177 mRNA. Translation: AAH19177.1.
BC022115 mRNA. Translation: AAH22115.1.
BC031153 mRNA. Translation: AAH31153.1.
CCDSiCCDS25751.1. [Q8VBT9-1]
CCDS25752.1. [Q8VBT9-2]
CCDS49008.1. [Q8VBT9-3]
RefSeqiNP_001157696.1. NM_001164224.1. [Q8VBT9-3]
NP_081153.1. NM_026877.2. [Q8VBT9-1]
NP_937866.1. NM_198223.2. [Q8VBT9-2]
XP_006534149.1. XM_006534086.2. [Q8VBT9-2]
XP_006534150.1. XM_006534087.2. [Q8VBT9-2]
UniGeneiMm.294020.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AL3NMR-A1-90[»]
ProteinModelPortaliQ8VBT9.
SMRiQ8VBT9. Positions 10-85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026135.

PTM databases

iPTMnetiQ8VBT9.
PhosphoSiteiQ8VBT9.

2D gel databases

REPRODUCTION-2DPAGEQ8VBT9.

Proteomic databases

EPDiQ8VBT9.
MaxQBiQ8VBT9.
PaxDbiQ8VBT9.
PRIDEiQ8VBT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026135; ENSMUSP00000026135; ENSMUSG00000025142. [Q8VBT9-1]
ENSMUST00000103016; ENSMUSP00000099305; ENSMUSG00000025142. [Q8VBT9-2]
ENSMUST00000106158; ENSMUSP00000101764; ENSMUSG00000025142. [Q8VBT9-3]
ENSMUST00000106159; ENSMUSP00000101765; ENSMUSG00000025142. [Q8VBT9-2]
ENSMUST00000106160; ENSMUSP00000101766; ENSMUSG00000025142. [Q8VBT9-3]
GeneIDi68938.
KEGGimmu:68938.
UCSCiuc007mua.2. mouse. [Q8VBT9-1]
uc007mub.1. mouse. [Q8VBT9-3]

Organism-specific databases

CTDi79058.
MGIiMGI:1916188. Aspscr1.

Phylogenomic databases

eggNOGiKOG2699. Eukaryota.
ENOG410XQQK. LUCA.
GeneTreeiENSGT00840000129867.
HOGENOMiHOG000231241.
HOVERGENiHBG066860.
InParanoidiQ8VBT9.
KOiK15627.
OMAiMIKNLKR.
OrthoDBiEOG7S7SF9.
PhylomeDBiQ8VBT9.
TreeFamiTF320363.

Miscellaneous databases

ChiTaRSiAspscr1. mouse.
EvolutionaryTraceiQ8VBT9.
NextBioi328221.
PROiQ8VBT9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VBT9.
ExpressionAtlasiQ8VBT9. baseline and differential.
GenevisibleiQ8VBT9. MM.

Family and domain databases

InterProiIPR021569. TUG-UBL1.
IPR029071. Ubiquitin-rel_dom.
IPR001012. UBX_dom.
[Graphical view]
PfamiPF11470. TUG-UBL1. 1 hit.
PF00789. UBX. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 2 hits.
PROSITEiPS50033. UBX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Functional cloning of TUG as a regulator of GLUT4 glucose transporter trafficking."
    Bogan J.S., Hendon N., McKee A.E., Tsao T.-S., Lodish H.F.
    Nature 425:727-733(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, INTERACTION WITH GLUT4, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Liver and Muscle.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
    Strain: C57BL/6J.
    Tissue: Embryo and Placenta.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Liver and Mammary tumor.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-193, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. "Solution structure and backbone dynamics of an N-terminal ubiquitin-like domain in the GLUT4-regulating protein, TUG."
    Tettamanzi M.C., Yu C., Bogan J.S., Hodsdon M.E.
    Protein Sci. 15:498-508(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 1-90.

Entry informationi

Entry nameiASPC1_MOUSE
AccessioniPrimary (citable) accession number: Q8VBT9
Secondary accession number(s): A2ABZ7
, A2AC06, Q3UKD1, Q6V7K5, Q9CT64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2006
Last sequence update: March 1, 2002
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.