Q8VA42 (RDRP_AQRVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase VP2
EC=2.7.7.48

Gene names

Name:

S2

Organism

Aquareovirus A (isolate Chum salmon/Japan/CSRV/1981) (AQRV-A)

Taxonomic identifier

928295 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Aquareovirus › Aquareovirus A

Protein attributes

Sequence length	1240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-directed RNA polymerase that is involved in transcription and genome replication. Following infection, it catalyzes the synthesis of fully conservative plus strands. After core assembly, which consists in recruitment of one capped plus-strand for each genomic segments and polymerase complexes, the polymerase switches mode and catalyzes the synthesis of complementary minus-strands By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Virion Potential.
Sequence similarities	Belongs to the reoviridae RNA-directed RNA polymerase family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Cellular component	Virion
Ligand	Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological_process	RNA biosynthetic process Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	viral nucleocapsid Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1240	1240	RNA-directed RNA polymerase VP2		PRO_0000404171
Regions
	Domain	516 – 764	249	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	Q8VA42 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7715D9DA5584D08D	FASTA	1,240	137,575
	10 20 30 40 50 60 MPGTYAPVPN VYHLLDHLTF RSSVVIPNSN FVGRIWSDYW ALQDNIVIRV SPEGAKDADY 70 80 90 100 110 120 CHNSNISPIL SPLKTITEYG TLHPTIDKDA SERGYPSARM ASSFFKLASC QARQVKIDPT 130 140 150 160 170 180 RFLEFLLVTA SSPRVPSGVD SDQPNPWLPE TSPALQAIWQ IMQRYKLNGN YYAPALVVNT 190 200 210 220 230 240 GAVWWIPPPG RTNCVTVQFL ITDLINLAVN AFATRLAPEL EMCAVRVYLA AASTPNYAHA 250 260 270 280 290 300 LLDLKSIFPN LSLHSMYREG EFGGKCPRIE WTEPRSSYRF KWIGVTQLYE GLRPLTPSRD 310 320 330 340 350 360 SKALLKMRDS GLEDVAKVII TMRRDHPRHT SDSVRFVRGV LSLISGMYLV RPPTMSVLRE 370 380 390 400 410 420 YSQTPQIEEP IPPDWWTGAV GNLSYFNDKA KGPLAHLYSV WLEAARQVVM DPSTHDPLTQ 430 440 450 460 470 480 AIYKTQFVTP RGGSSAALKQ ALVESKVELP DFSGTGVKRS SKIYQTAQLA HFSFQTLIPT 490 500 510 520 530 540 IMGQVTLGIR NQVQRRARSI MPMSNPQQTV SVPHTLVANY INKHMNRSTT SGSAVQDKVI 550 560 570 580 590 600 PLLLYASTPP RTVINVDIKA CDASITYAAF LAPICGAMHQ GFDLGDPSLP FMNVPSSTQY 610 620 630 640 650 660 DRRNPAAPYN RPVSGLQTMT QHLARLYQAG FSYKVDDPFS SGNSFVFPTT TFPSGSTATS 670 680 690 700 710 720 TEHTANNGAM ADFFLREYVP QHAKSSTLKF IVKDMNIQNN YVCQGDDGML IIPDLGTKRI 730 740 750 760 770 780 SPEDLAELME LLEKYGRGFG WVYDIDSSDS AEYLKLYALF GARIPNISRH PPVGKEYASP 790 800 810 820 830 840 ETGEIWPSLV NIAMGSFYNG VTDCLEWRDW LKFSWAFACF ASRGSFHPKT GPRVDAQYPV 850 860 870 880 890 900 WSFIYMGLPP ILLPGQTPFL TSVYMPAGDQ GIFAILHQWR DYLTARATSD YPPLKRRHPV 910 920 930 940 950 960 WHLADVPSLL SDLGVYRGYW AAQVSRRPEP SPDDADPASV EAMSAALSTY LLKDPVLRDR 970 980 990 1000 1010 1020 VVRGTNAWRR LTDTHPGRLP SRVPSLLDVP TRWIKAGRDA DKPRPSAVAM MMKDIQRAAS 1030 1040 1050 1060 1070 1080 SSRKDFSRLL ELYLHVHVHL GPPVPLAVDP EVPHVAGADI LNDDHWYKVT SLGPIAQSTK 1090 1100 1110 1120 1130 1140 KYFDATLFVG KTVSGLDVEA VDATLLRMSI LGAEPEEYHA FLAGIGMSDA EAHRIASAIS 1150 1160 1170 1180 1190 1200 LADAQIVQLA RTVNLAVPSS WMSLDFDTLI RSHSYPRQPG ISDSSTLVRE RASWINSVLR 1210 1220 1230 1240 LLCATVAMSR VGPVCQATVS SVDGGVNQIV GCLRAWMRDV

References

[1]	"Morphological and biochemical properties of four members of a novel group of reoviruses isolated from aquatic animals." Winton J.R., Lannan C.N., Fryer J.L., Hedrick R.P., Meyers T.R., Plumb J.A., Yamamoto T. J. Gen. Virol. 68:353-364(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Complete genome sequence of the chum salmon virus." Rao S., Carner G.R., Chen W., Winton J.R. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF418295 Genomic RNA. Translation: AAL31497.1.
RefSeq	YP_398630.1. NC_007583.1.
3D structure databases
HSSP	HSSP built from PDB template 1N35 based on UniProtKB P17378.
ProteinModelPortal	Q8VA42.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3773156.
Family and domain databases
InterPro	IPR012915. RdRP_5. IPR007097. RNA-dir_pol_reovirus. [Graphical view]
Pfam	PF07925. RdRP_5. 1 hit. [Graphical view]
PROSITE	PS50523. RDRP_DSRNA_REO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_AQRVA

Accession

Primary (citable) accession number: Q8VA42

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 8, 2011
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program