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Q8V9C4

- NSP5_ROTHJ

UniProt

Q8V9C4 - NSP5_ROTHJ

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Protein
Non-structural protein 5
Gene
N/A
Organism
Rotavirus A (strain Human/Japan/K8/1977 G1-P3A[9]-Ix-Rx-Cx-Mx-A1-Nx-Tx-Ex-H3) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in genome replication. Plays a crucial role, together with NSP2, in the formation of virus factories (viroplasms) which are large inclusions in the cytoplasm where core-like replication intermediates are assembled and RNA replication takes place. May regulate NSP2-RNA interactions during genome replication, since NSP5 competes with RNA for the same binding site on the NSP2 octamer. Binds to either ssRNA or dsRNA with similar affinities. Displays ATPase and autokinase activities By similarity.

Cofactori

Magnesium for ATPase activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Magnesium Reviewed prediction

GO - Molecular functioni

  1. ATPase activity Source: InterPro
  2. RNA binding Source: UniProtKB-KW
  3. magnesium ion binding Source: InterPro
  4. nucleotide binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. viral genome replication Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 5
Short name:
NSP5
Alternative name(s):
NS26
OrganismiRotavirus A (strain Human/Japan/K8/1977 G1-P3A[9]-Ix-Rx-Cx-Mx-A1-Nx-Tx-Ex-H3) (RV-A)
Taxonomic identifieri39012 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]

Subcellular locationi

Host cytoplasm
Note: Found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging By similarity.

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Non-structural protein 5
PRO_0000369500Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671Phosphoserine; by host CK1 By similarity
Modified residuei154 – 1541Phosphoserine; by host By similarity
Modified residuei156 – 1561Phosphoserine; by host By similarity
Modified residuei164 – 1641Phosphoserine; by host By similarity
Modified residuei166 – 1661Phosphoserine; by host By similarity

Post-translational modificationi

O-glycosylated By similarity.
Hyperphosphorylated on serine residues, when in dimeric form. Ser-67 phosphorylation by CK1 is required for the hyperphosphorylation of NSP5 dimer. Impaired phosphorylation is associated with a profound morphological change in virus factories and a moderate decrease in virus replication By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer. Interacts with VP1. Interacts with VP2. Interacts with NSP2; this interaction leads to up-regulation of NSP5 hyperphosphorylation and formation of virus factories. Interacts with NSP6 By similarity.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4848Interaction with VP1 By similarity
Add
BLAST
Regioni189 – 19810Homodimerization and interaction with NSP6 By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 101100Ser-rich
Add
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP5 family.

Family and domain databases

InterProiIPR002512. Rotavirus_A/C_NSP5.
[Graphical view]
PfamiPF01525. Rota_NS26. 1 hit.
[Graphical view]
PIRSFiPIRSF004006. Rota_NS26. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8V9C4-1 [UniParc]FASTAAdd to Basket

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MSLSIDVTSL PSISSSIYKN ESSSTTSTLS GKSIGRSEQY ISPDAEAFSK    50
YMLSKSPEDI GPSDSASNDP LTSFSNRSNA VKTNADAGVS MDSSTQSRPS 100
SNVGCDQVDF SFNKGIKVSA NLDSSVSIST NVKKEKSKND HRSRKHYPKI 150
EAESDSDDYV LDDSDSDDGK CKNCKYKRKY FALRMRMKRV AMQLIEDL 198
Length:198
Mass (Da):21,721
Last modified:March 1, 2002 - v1
Checksum:i78053A892E043018
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB008655 mRNA. Translation: BAB83812.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB008655 mRNA. Translation: BAB83812.1 .

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002512. Rotavirus_A/C_NSP5.
[Graphical view ]
Pfami PF01525. Rota_NS26. 1 hit.
[Graphical view ]
PIRSFi PIRSF004006. Rota_NS26. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Serological and genomic characterization of human rotaviruses detected in China."
    Wu H., Taniguchi K., Urasawa T., Urasawa S.
    J. Med. Virol. 55:168-176(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNSP5_ROTHJ
AccessioniPrimary (citable) accession number: Q8V9C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: March 1, 2002
Last modified: April 3, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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