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Q8V736

- POLG_CACV4

UniProt

Q8V736 - POLG_CACV4

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Protein
Genome polyprotein
Gene
ORF1
Organism
Canine calicivirus (strain 48) (CaCV)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity By similarity.
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.
Protease-polymerase processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved. Cleaves host translation initiation factor eIF4G1 and eIF4G2 thereby inducing a shutdown of host protein synthesis. This shutdown may not prevent viral mRNA from being translated since viral Vpg replaces the cap. May cleave host polyadenylate-binding protein thereby inhibiting cellular translation. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.

Catalytic activityi

NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei176 – 1772Cleavage; by Pro-Pol By similarity
Sitei468 – 4692Cleavage; by Pro-Pol By similarity
Sitei825 – 8262Cleavage; by Pro-Pol By similarity
Sitei1111 – 11122Cleavage; by Pro-Pol By similarity
Sitei1232 – 12332Cleavage; by Pro-Pol By similarity
Active sitei1271 – 12711For protease activity By similarity
Active sitei1292 – 12921For protease activity By similarity
Active sitei1355 – 13551For protease activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi623 – 6308ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: InterPro
  3. RNA helicase activity Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. cysteine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Host-virus interaction, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 6 chains:
Alternative name(s):
p39
Alternative name(s):
VPg
p13
Protease-polymerase (EC:2.7.7.48, EC:3.4.22.66)
Short name:
Pro-Pol
Gene namesi
ORF Names:ORF1
OrganismiCanine calicivirus (strain 48) (CaCV)
Taxonomic identifieri292348 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridae
Virus hostiCanis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19291929Genome polyprotein
PRO_0000341984Add
BLAST
Chaini1 – 176176Protein p18
PRO_0000341985Add
BLAST
Chaini177 – 468292Protein p32 By similarity
PRO_0000036886Add
BLAST
Chaini469 – 825357NTPase By similarity
PRO_0000036887Add
BLAST
Chaini826 – 1112287Protein p30 By similarity
PRO_0000036888Add
BLAST
Chaini1113 – 1232120Viral genome-linked protein By similarity
PRO_0000036889Add
BLAST
Chaini1233 – 1879647Protease-polymerase By similarity
PRO_0000036891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1137 – 11371O-(5'-phospho-RNA)-tyrosine By similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein By similarity.
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ8V736.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini597 – 753157SF3 helicase
Add
BLAST
Domaini1256 – 1361106Peptidase C24
Add
BLAST
Domaini1643 – 1768126RdRp catalytic
Add
BLAST

Domaini

Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently By similarity.

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8V736-1 [UniParc]FASTAAdd to Basket

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MASAIALSSS TAQNKITLKS VASRLQQTDD PDIRVWSQSV GFHLQFSNWK     50
CANAFCRFVT DAYNLTPYKE CARSITRQLT SLSNYLSAQT GVSVSGTQFL 100
LSPSDVEVPV AKTGESVSDI MVPSYSVNGT SMEFDSMAQL AQALTTGFTF 150
SVNDAQIGNA PAQTGESVSG TGFIAEACPS CALYDKCPNC TSELINDDGS 200
SQSPGDIPHW THHKIASGIV NILSSDMSSM EDDDFANIAA HVKKALGTNS 250
HPANNDMSKD QLNWLLNIAE ASLIRKADRT ALPMNAARIA ARRGWREKLF 300
NEPADKLYTL LRKSKDSFQK SAIWGILFEK ASNAKHYTEI VFQDIVKLIK 350
EECNPSNNFY FKVMAQSFLD HFRMLVIDNP DPVANLPKFI LKLKPLNLKM 400
IIENHENTAE GWIVTLTAVA ELYGWLEFAV DLVPKIVSEL YDLLTSATQK 450
CFSMVRELLT NLNILKAESF DFTNPFWYAL AALLSYFVTG FLPNNAKCSA 500
IKQTLNGATT LVAGITAIQK LAAMFSAWSN ESVVDDLSTK VIGLTEADNP 550
TVTQDIDAVT NLQIMAEQLK DQIKLKTLDP TFQPYLPVLR NLMSTTDSVI 600
SHCAKRKALA TQRTAPVCII LTGPAGCGKT TLAYAIANRL SAQKPSVLNL 650
NIDHHDAYTG NEVCIIDEFD SNPDSKFVEF VVEMVNTNPM LLNCDLIENK 700
GKTFSSKYVI MTSNNETPVK PNSTRAPPFY RRVRIIDVTN PGVMSFKYEN 750
PGQEVPSYLF SNDFNHLSMS MRGFGAFSKT RVIDPEGRKT CGLEGPPGQR 800
VDVDDIVRYM QRMYRENQMN FKSEAGNNRL KTPRFAFVTQ RKHVDTVYKI 850
LAAAKTTYNG YYSLTKDSFD VNEGHNIGSS VFVVGDDKEI PHNCKIFRCN 900
HLAMFRHPEL AHIEGDNFRA ALGVTMSDQD VTLMFYHIRG KHIQDEVRLD 950
ELPANHHIVT VHSVYDMAWA LNRHLSLTGK WQALKAVYDL YMTPDILPAA 1000
LRHWMDNTKF SSDHVVTQFI VPGGTIILET CNGARMWATS RRLIRAGGIS 1050
NNNGPEGGFR FGSIAPRDIP WSEILREFLN LISLIWSRVK GATIVLTALL 1100
LYMKRYKPRS EAKGKTKGGR GAIRHGGKGI VLSDDEYDEW REFNMEKRMD 1150
MSVDEFLMLK HRAALGSDDT GAIQFRSWWT ARQMRESTGL DHDDVTVIGK 1200
GGVRHEVHRT EIMKAPKQKK KSFAWGEDMY AEGDGKIVNH VNAIVPVTGL 1250
CGEHIGYAVH IGHGKCISLK HVLKTGSYVF NQKPIDVTFD GELAHFQIQQ 1300
PPSSAAPVTF SSKPTRDPWG RSVSTEWKHD TYNTTAGKMY GSICWTATRT 1350
QPGDCGLPYV DRAGQVVGLH AGSGGDSAPG RKIVIPVTKF KLPSNTVLSN 1400
RFWKEEAPTI SYKGLTVQET GVNKAVLKGT NYHVSPAHVD DYQDCTHQPA 1450
NLGAQDERYP VSLTSIVINN LEPYKQPTQG PPTEVLNKAY NMLVQHYEPL 1500
IPKATTHLEM GDAFAALNVK TSCGPYITGR KKDHIDPETG KWDETLRNHI 1550
NARWSLATQG VPIPHEYQLG LKDELRPKDK IAVGKRRLIW GCDVGVAVVA 1600
ASAFKEVSSA IMAMSEFDFI QVGINMDGTA VETLYKRLYT PGTHRYCVDY 1650
SKWDSTQPPN VTRMSLELLR HFTDKSPVVD SAVATLSSPS IAVFGGVSFK 1700
TNGGLPSGMP LTSILNSLNH CLLVGSAIIQ VLESKGVDVN WNIYDTIDLF 1750
TYGDDGVYIV PNFVHSVMPE VFSCLSSYGL KPTRTDKSSA PITEIPLSEP 1800
IEFLKRQFVR NQFGVRALLD RSSLIRQFYY IKGKNTMEWT KPPEQIDLTS 1850
RTAQLQVVML YASQHGREFY KKCLDYYQLA MEYEGIKLDA PTYDEALAKY 1900
NANFNGVEDC DLLPAGYDEH RLDKIVFEN 1929
Length:1,929
Mass (Da):214,806
Last modified:March 1, 2002 - v1
Checksum:i9F8A92FC0657A623
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB070225 Genomic RNA. Translation: BAB83601.1.
AF053720 Genomic RNA. Translation: AAC16445.1.
RefSeqiNP_777373.1. NC_004542.1.

Genome annotation databases

GeneIDi956316.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB070225 Genomic RNA. Translation: BAB83601.1 .
AF053720 Genomic RNA. Translation: AAC16445.1 .
RefSeqi NP_777373.1. NC_004542.1.

3D structure databases

ProteinModelPortali Q8V736.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956316.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR003593. AAA+_ATPase.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus."
    Matsuura Y., Tohya Y., Nakamura K., Shimojima M., Roerink F., Mochizuki M., Takase K., Akashi H., Sugimura T.
    Virus Genes 25:67-73(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Organization of the canine calicivirus genome from the RNA polymerase gene to the poly(A) tail."
    Roerink F., Hashimoto M., Tohya Y., Mochizuki M.
    J. Gen. Virol. 80:929-935(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1571-1929.

Entry informationi

Entry nameiPOLG_CACV4
AccessioniPrimary (citable) accession number: Q8V736
Secondary accession number(s): O72119
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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