Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8V736

- POLG_CACV4

UniProt

Q8V736 - POLG_CACV4

Protein

Genome polyprotein

Gene

ORF1

Organism
Canine calicivirus (strain 48) (CaCV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity By similarity.By similarity
    Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation By similarity.By similarity
    Protease-polymerase processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved. Cleaves host translation initiation factor eIF4G1 and eIF4G2 thereby inducing a shutdown of host protein synthesis. This shutdown may not prevent viral mRNA from being translated since viral Vpg replaces the cap. May cleave host polyadenylate-binding protein thereby inhibiting cellular translation. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs By similarity.By similarity

    Catalytic activityi

    NTP + H2O = NDP + phosphate.
    Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei176 – 1772Cleavage; by Pro-PolBy similarity
    Sitei468 – 4692Cleavage; by Pro-PolBy similarity
    Sitei825 – 8262Cleavage; by Pro-PolBy similarity
    Sitei1111 – 11122Cleavage; by Pro-PolBy similarity
    Sitei1232 – 12332Cleavage; by Pro-PolBy similarity
    Active sitei1271 – 12711For protease activityBy similarity
    Active sitei1292 – 12921For protease activityBy similarity
    Active sitei1355 – 13551For protease activityBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi623 – 6308ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cysteine-type endopeptidase activity Source: InterPro
    3. RNA binding Source: InterPro
    4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
    5. RNA helicase activity Source: InterPro

    GO - Biological processi

    1. RNA-protein covalent cross-linking Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro
    3. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

    Keywords - Biological processi

    Host-virus interaction, Viral RNA replication

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Genome polyprotein
    Cleaved into the following 6 chains:
    Alternative name(s):
    p39
    Alternative name(s):
    VPg
    p13
    Protease-polymerase (EC:2.7.7.48, EC:3.4.22.66)
    Short name:
    Pro-Pol
    Gene namesi
    ORF Names:ORF1
    OrganismiCanine calicivirus (strain 48) (CaCV)
    Taxonomic identifieri292348 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridae
    Virus hostiCanis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 19291929Genome polyproteinPRO_0000341984Add
    BLAST
    Chaini1 – 176176Protein p18PRO_0000341985Add
    BLAST
    Chaini177 – 468292Protein p32By similarityPRO_0000036886Add
    BLAST
    Chaini469 – 825357NTPaseBy similarityPRO_0000036887Add
    BLAST
    Chaini826 – 1112287Protein p30By similarityPRO_0000036888Add
    BLAST
    Chaini1113 – 1232120Viral genome-linked proteinBy similarityPRO_0000036889Add
    BLAST
    Chaini1233 – 1879647Protease-polymeraseBy similarityPRO_0000036891Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1137 – 11371O-(5'-phospho-RNA)-tyrosineBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein By similarity.By similarity
    VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.By similarity

    Keywords - PTMi

    Covalent protein-RNA linkage, Phosphoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ8V736.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini597 – 753157SF3 helicasePROSITE-ProRule annotationAdd
    BLAST
    Domaini1256 – 1361106Peptidase C24Add
    BLAST
    Domaini1643 – 1768126RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently By similarity.By similarity

    Sequence similaritiesi

    Contains 1 peptidase C24 domain.Curated
    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
    Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF03510. Peptidase_C24. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view]
    PRINTSiPR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8V736-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASAIALSSS TAQNKITLKS VASRLQQTDD PDIRVWSQSV GFHLQFSNWK     50
    CANAFCRFVT DAYNLTPYKE CARSITRQLT SLSNYLSAQT GVSVSGTQFL 100
    LSPSDVEVPV AKTGESVSDI MVPSYSVNGT SMEFDSMAQL AQALTTGFTF 150
    SVNDAQIGNA PAQTGESVSG TGFIAEACPS CALYDKCPNC TSELINDDGS 200
    SQSPGDIPHW THHKIASGIV NILSSDMSSM EDDDFANIAA HVKKALGTNS 250
    HPANNDMSKD QLNWLLNIAE ASLIRKADRT ALPMNAARIA ARRGWREKLF 300
    NEPADKLYTL LRKSKDSFQK SAIWGILFEK ASNAKHYTEI VFQDIVKLIK 350
    EECNPSNNFY FKVMAQSFLD HFRMLVIDNP DPVANLPKFI LKLKPLNLKM 400
    IIENHENTAE GWIVTLTAVA ELYGWLEFAV DLVPKIVSEL YDLLTSATQK 450
    CFSMVRELLT NLNILKAESF DFTNPFWYAL AALLSYFVTG FLPNNAKCSA 500
    IKQTLNGATT LVAGITAIQK LAAMFSAWSN ESVVDDLSTK VIGLTEADNP 550
    TVTQDIDAVT NLQIMAEQLK DQIKLKTLDP TFQPYLPVLR NLMSTTDSVI 600
    SHCAKRKALA TQRTAPVCII LTGPAGCGKT TLAYAIANRL SAQKPSVLNL 650
    NIDHHDAYTG NEVCIIDEFD SNPDSKFVEF VVEMVNTNPM LLNCDLIENK 700
    GKTFSSKYVI MTSNNETPVK PNSTRAPPFY RRVRIIDVTN PGVMSFKYEN 750
    PGQEVPSYLF SNDFNHLSMS MRGFGAFSKT RVIDPEGRKT CGLEGPPGQR 800
    VDVDDIVRYM QRMYRENQMN FKSEAGNNRL KTPRFAFVTQ RKHVDTVYKI 850
    LAAAKTTYNG YYSLTKDSFD VNEGHNIGSS VFVVGDDKEI PHNCKIFRCN 900
    HLAMFRHPEL AHIEGDNFRA ALGVTMSDQD VTLMFYHIRG KHIQDEVRLD 950
    ELPANHHIVT VHSVYDMAWA LNRHLSLTGK WQALKAVYDL YMTPDILPAA 1000
    LRHWMDNTKF SSDHVVTQFI VPGGTIILET CNGARMWATS RRLIRAGGIS 1050
    NNNGPEGGFR FGSIAPRDIP WSEILREFLN LISLIWSRVK GATIVLTALL 1100
    LYMKRYKPRS EAKGKTKGGR GAIRHGGKGI VLSDDEYDEW REFNMEKRMD 1150
    MSVDEFLMLK HRAALGSDDT GAIQFRSWWT ARQMRESTGL DHDDVTVIGK 1200
    GGVRHEVHRT EIMKAPKQKK KSFAWGEDMY AEGDGKIVNH VNAIVPVTGL 1250
    CGEHIGYAVH IGHGKCISLK HVLKTGSYVF NQKPIDVTFD GELAHFQIQQ 1300
    PPSSAAPVTF SSKPTRDPWG RSVSTEWKHD TYNTTAGKMY GSICWTATRT 1350
    QPGDCGLPYV DRAGQVVGLH AGSGGDSAPG RKIVIPVTKF KLPSNTVLSN 1400
    RFWKEEAPTI SYKGLTVQET GVNKAVLKGT NYHVSPAHVD DYQDCTHQPA 1450
    NLGAQDERYP VSLTSIVINN LEPYKQPTQG PPTEVLNKAY NMLVQHYEPL 1500
    IPKATTHLEM GDAFAALNVK TSCGPYITGR KKDHIDPETG KWDETLRNHI 1550
    NARWSLATQG VPIPHEYQLG LKDELRPKDK IAVGKRRLIW GCDVGVAVVA 1600
    ASAFKEVSSA IMAMSEFDFI QVGINMDGTA VETLYKRLYT PGTHRYCVDY 1650
    SKWDSTQPPN VTRMSLELLR HFTDKSPVVD SAVATLSSPS IAVFGGVSFK 1700
    TNGGLPSGMP LTSILNSLNH CLLVGSAIIQ VLESKGVDVN WNIYDTIDLF 1750
    TYGDDGVYIV PNFVHSVMPE VFSCLSSYGL KPTRTDKSSA PITEIPLSEP 1800
    IEFLKRQFVR NQFGVRALLD RSSLIRQFYY IKGKNTMEWT KPPEQIDLTS 1850
    RTAQLQVVML YASQHGREFY KKCLDYYQLA MEYEGIKLDA PTYDEALAKY 1900
    NANFNGVEDC DLLPAGYDEH RLDKIVFEN 1929
    Length:1,929
    Mass (Da):214,806
    Last modified:March 1, 2002 - v1
    Checksum:i9F8A92FC0657A623
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB070225 Genomic RNA. Translation: BAB83601.1.
    AF053720 Genomic RNA. Translation: AAC16445.1.
    RefSeqiNP_777373.1. NC_004542.1.

    Genome annotation databases

    GeneIDi956316.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB070225 Genomic RNA. Translation: BAB83601.1 .
    AF053720 Genomic RNA. Translation: AAC16445.1 .
    RefSeqi NP_777373.1. NC_004542.1.

    3D structure databases

    ProteinModelPortali Q8V736.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956316.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR004004. Helic/Pol/Pept_Calicivir-typ.
    IPR000605. Helicase_SF3_ssDNA/RNA_vir.
    IPR014759. Helicase_SF3_ssRNA_vir.
    IPR027417. P-loop_NTPase.
    IPR000317. Peptidase_C24.
    IPR001205. RNA-dir_pol_C.
    IPR007094. RNA-dir_pol_PSvirus.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF03510. Peptidase_C24. 1 hit.
    PF00680. RdRP_1. 1 hit.
    PF00910. RNA_helicase. 1 hit.
    [Graphical view ]
    PRINTSi PR00916. 2CENDOPTASE.
    PR00918. CALICVIRUSNS.
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
    PS51218. SF3_HELICASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus."
      Matsuura Y., Tohya Y., Nakamura K., Shimojima M., Roerink F., Mochizuki M., Takase K., Akashi H., Sugimura T.
      Virus Genes 25:67-73(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Organization of the canine calicivirus genome from the RNA polymerase gene to the poly(A) tail."
      Roerink F., Hashimoto M., Tohya Y., Mochizuki M.
      J. Gen. Virol. 80:929-935(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1571-1929.

    Entry informationi

    Entry nameiPOLG_CACV4
    AccessioniPrimary (citable) accession number: Q8V736
    Secondary accession number(s): O72119
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 9, 2004
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3