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Q8V736

- POLG_CACV4

UniProt

Q8V736 - POLG_CACV4

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Protein

Genome polyprotein

Gene

ORF1

Organism
Canine calicivirus (strain 48) (CaCV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

NTPase presumably plays a role in replication. Despite having similarities with helicases, does not seem to display any helicase activity (By similarity).By similarity
Viral genome-linked protein is covalently linked to the 5'-end of the positive-strand, negative-strand genomic RNAs and subgenomic RNA. Acts as a genome-linked replication primer. May recruit ribosome to viral RNA thereby promoting viral proteins translation (By similarity).By similarity
Protease-polymerase processes the polyprotein: Pro-Pol is first released by autocleavage, then all other proteins are cleaved. Cleaves host translation initiation factor eIF4G1 and eIF4G2 thereby inducing a shutdown of host protein synthesis. This shutdown may not prevent viral mRNA from being translated since viral Vpg replaces the cap. May cleave host polyadenylate-binding protein thereby inhibiting cellular translation. It is also an RNA-directed RNA polymerase which replicates genomic and antigenomic viral RNA by recognizing specific signals. Transcribes also a subgenomic mRNA by initiating RNA synthesis internally on antigenomic RNA. This sgRNA codes for structural proteins. Catalyzes the covalent attachment VPg with viral RNAs (By similarity).By similarity

Catalytic activityi

NTP + H2O = NDP + phosphate.
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|-Xaa and the P1' position is occupied by Gly-|-Yaa.
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei176 – 1772Cleavage; by Pro-PolBy similarity
Sitei468 – 4692Cleavage; by Pro-PolBy similarity
Sitei825 – 8262Cleavage; by Pro-PolBy similarity
Sitei1111 – 11122Cleavage; by Pro-PolBy similarity
Sitei1232 – 12332Cleavage; by Pro-PolBy similarity
Active sitei1271 – 12711For protease activityBy similarity
Active sitei1292 – 12921For protease activityBy similarity
Active sitei1355 – 13551For protease activityBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi623 – 6308ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. RNA binding Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. RNA helicase activity Source: InterPro

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase

Keywords - Biological processi

Host-virus interaction, Viral RNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

MEROPSiC24.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 6 chains:
Alternative name(s):
p39
Alternative name(s):
VPg
p13
Protease-polymerase (EC:2.7.7.48, EC:3.4.22.66)
Short name:
Pro-Pol
Gene namesi
ORF Names:ORF1
OrganismiCanine calicivirus (strain 48) (CaCV)
Taxonomic identifieri292348 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageCaliciviridae
Virus hostiCanis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19291929Genome polyproteinPRO_0000341984Add
BLAST
Chaini1 – 176176Protein p18PRO_0000341985Add
BLAST
Chaini177 – 468292Protein p32By similarityPRO_0000036886Add
BLAST
Chaini469 – 825357NTPaseBy similarityPRO_0000036887Add
BLAST
Chaini826 – 1112287Protein p30By similarityPRO_0000036888Add
BLAST
Chaini1113 – 1232120Viral genome-linked proteinBy similarityPRO_0000036889Add
BLAST
Chaini1233 – 1879647Protease-polymeraseBy similarityPRO_0000036891Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1137 – 11371O-(5'-phospho-RNA)-tyrosineBy similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. Pro-Pol is first autocatalytically cleaved, then processes the whole polyprotein (By similarity).By similarity
VPg is uridylylated by the polymerase and is covalently attached to the 5'-end of the polyadenylated genomic and subgenomic RNAs. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity

Keywords - PTMi

Covalent protein-RNA linkage, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ8V736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini597 – 753157SF3 helicasePROSITE-ProRule annotationAdd
BLAST
Domaini1256 – 1361106Peptidase C24Add
BLAST
Domaini1643 – 1768126RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Domaini

Protease-polymerase is composed of two domains displaying two different catalytic activity. These activities may act independently (By similarity).By similarity

Sequence similaritiesi

Contains 1 peptidase C24 domain.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR003593. AAA+_ATPase.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8V736-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MASAIALSSS TAQNKITLKS VASRLQQTDD PDIRVWSQSV GFHLQFSNWK
60 70 80 90 100
CANAFCRFVT DAYNLTPYKE CARSITRQLT SLSNYLSAQT GVSVSGTQFL
110 120 130 140 150
LSPSDVEVPV AKTGESVSDI MVPSYSVNGT SMEFDSMAQL AQALTTGFTF
160 170 180 190 200
SVNDAQIGNA PAQTGESVSG TGFIAEACPS CALYDKCPNC TSELINDDGS
210 220 230 240 250
SQSPGDIPHW THHKIASGIV NILSSDMSSM EDDDFANIAA HVKKALGTNS
260 270 280 290 300
HPANNDMSKD QLNWLLNIAE ASLIRKADRT ALPMNAARIA ARRGWREKLF
310 320 330 340 350
NEPADKLYTL LRKSKDSFQK SAIWGILFEK ASNAKHYTEI VFQDIVKLIK
360 370 380 390 400
EECNPSNNFY FKVMAQSFLD HFRMLVIDNP DPVANLPKFI LKLKPLNLKM
410 420 430 440 450
IIENHENTAE GWIVTLTAVA ELYGWLEFAV DLVPKIVSEL YDLLTSATQK
460 470 480 490 500
CFSMVRELLT NLNILKAESF DFTNPFWYAL AALLSYFVTG FLPNNAKCSA
510 520 530 540 550
IKQTLNGATT LVAGITAIQK LAAMFSAWSN ESVVDDLSTK VIGLTEADNP
560 570 580 590 600
TVTQDIDAVT NLQIMAEQLK DQIKLKTLDP TFQPYLPVLR NLMSTTDSVI
610 620 630 640 650
SHCAKRKALA TQRTAPVCII LTGPAGCGKT TLAYAIANRL SAQKPSVLNL
660 670 680 690 700
NIDHHDAYTG NEVCIIDEFD SNPDSKFVEF VVEMVNTNPM LLNCDLIENK
710 720 730 740 750
GKTFSSKYVI MTSNNETPVK PNSTRAPPFY RRVRIIDVTN PGVMSFKYEN
760 770 780 790 800
PGQEVPSYLF SNDFNHLSMS MRGFGAFSKT RVIDPEGRKT CGLEGPPGQR
810 820 830 840 850
VDVDDIVRYM QRMYRENQMN FKSEAGNNRL KTPRFAFVTQ RKHVDTVYKI
860 870 880 890 900
LAAAKTTYNG YYSLTKDSFD VNEGHNIGSS VFVVGDDKEI PHNCKIFRCN
910 920 930 940 950
HLAMFRHPEL AHIEGDNFRA ALGVTMSDQD VTLMFYHIRG KHIQDEVRLD
960 970 980 990 1000
ELPANHHIVT VHSVYDMAWA LNRHLSLTGK WQALKAVYDL YMTPDILPAA
1010 1020 1030 1040 1050
LRHWMDNTKF SSDHVVTQFI VPGGTIILET CNGARMWATS RRLIRAGGIS
1060 1070 1080 1090 1100
NNNGPEGGFR FGSIAPRDIP WSEILREFLN LISLIWSRVK GATIVLTALL
1110 1120 1130 1140 1150
LYMKRYKPRS EAKGKTKGGR GAIRHGGKGI VLSDDEYDEW REFNMEKRMD
1160 1170 1180 1190 1200
MSVDEFLMLK HRAALGSDDT GAIQFRSWWT ARQMRESTGL DHDDVTVIGK
1210 1220 1230 1240 1250
GGVRHEVHRT EIMKAPKQKK KSFAWGEDMY AEGDGKIVNH VNAIVPVTGL
1260 1270 1280 1290 1300
CGEHIGYAVH IGHGKCISLK HVLKTGSYVF NQKPIDVTFD GELAHFQIQQ
1310 1320 1330 1340 1350
PPSSAAPVTF SSKPTRDPWG RSVSTEWKHD TYNTTAGKMY GSICWTATRT
1360 1370 1380 1390 1400
QPGDCGLPYV DRAGQVVGLH AGSGGDSAPG RKIVIPVTKF KLPSNTVLSN
1410 1420 1430 1440 1450
RFWKEEAPTI SYKGLTVQET GVNKAVLKGT NYHVSPAHVD DYQDCTHQPA
1460 1470 1480 1490 1500
NLGAQDERYP VSLTSIVINN LEPYKQPTQG PPTEVLNKAY NMLVQHYEPL
1510 1520 1530 1540 1550
IPKATTHLEM GDAFAALNVK TSCGPYITGR KKDHIDPETG KWDETLRNHI
1560 1570 1580 1590 1600
NARWSLATQG VPIPHEYQLG LKDELRPKDK IAVGKRRLIW GCDVGVAVVA
1610 1620 1630 1640 1650
ASAFKEVSSA IMAMSEFDFI QVGINMDGTA VETLYKRLYT PGTHRYCVDY
1660 1670 1680 1690 1700
SKWDSTQPPN VTRMSLELLR HFTDKSPVVD SAVATLSSPS IAVFGGVSFK
1710 1720 1730 1740 1750
TNGGLPSGMP LTSILNSLNH CLLVGSAIIQ VLESKGVDVN WNIYDTIDLF
1760 1770 1780 1790 1800
TYGDDGVYIV PNFVHSVMPE VFSCLSSYGL KPTRTDKSSA PITEIPLSEP
1810 1820 1830 1840 1850
IEFLKRQFVR NQFGVRALLD RSSLIRQFYY IKGKNTMEWT KPPEQIDLTS
1860 1870 1880 1890 1900
RTAQLQVVML YASQHGREFY KKCLDYYQLA MEYEGIKLDA PTYDEALAKY
1910 1920
NANFNGVEDC DLLPAGYDEH RLDKIVFEN
Length:1,929
Mass (Da):214,806
Last modified:March 1, 2002 - v1
Checksum:i9F8A92FC0657A623
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB070225 Genomic RNA. Translation: BAB83601.1.
AF053720 Genomic RNA. Translation: AAC16445.1.
RefSeqiNP_777373.1. NC_004542.1.

Genome annotation databases

GeneIDi956316.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB070225 Genomic RNA. Translation: BAB83601.1 .
AF053720 Genomic RNA. Translation: AAC16445.1 .
RefSeqi NP_777373.1. NC_004542.1.

3D structure databases

ProteinModelPortali Q8V736.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C24.002.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956316.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR003593. AAA+_ATPase.
IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000317. Peptidase_C24.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF03510. Peptidase_C24. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00916. 2CENDOPTASE.
PR00918. CALICVIRUSNS.
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus."
    Matsuura Y., Tohya Y., Nakamura K., Shimojima M., Roerink F., Mochizuki M., Takase K., Akashi H., Sugimura T.
    Virus Genes 25:67-73(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Organization of the canine calicivirus genome from the RNA polymerase gene to the poly(A) tail."
    Roerink F., Hashimoto M., Tohya Y., Mochizuki M.
    J. Gen. Virol. 80:929-935(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1571-1929.

Entry informationi

Entry nameiPOLG_CACV4
AccessioniPrimary (citable) accession number: Q8V736
Secondary accession number(s): O72119
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3