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Protein

Spike glycoprotein

Gene

S

Organism
Bovine coronavirus (strain 98TXSF-110-LUN) (BCoV-LUN) (BCV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

S1 attaches the virion to the cell membrane by binding to 9-O-acetylated sialic acid containing proteins, initiating the infection.By similarity
S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processFusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virulence, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Spike glycoprotein
Short name:
S glycoprotein
Alternative name(s):
E2
Peplomer protein
Cleaved into the following 2 chains:
Alternative name(s):
90B
Alternative name(s):
90A
Gene namesi
Name:S
ORF Names:3
OrganismiBovine coronavirus (strain 98TXSF-110-LUN) (BCoV-LUN) (BCV)
Taxonomic identifieri233264 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000008571 Componenti: Genome

Subcellular locationi

Spike protein S2 :
Spike protein S1 :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini18 – 1311ExtracellularSequence analysisAdd BLAST1294
Transmembranei1312 – 1328HelicalSequence analysisAdd BLAST17
Topological domaini1329 – 1363CytoplasmicSequence analysisAdd BLAST35

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000028391318 – 1363Spike glycoproteinBy similarityAdd BLAST1346
ChainiPRO_000028391418 – 768Spike protein S1By similarityAdd BLAST751
ChainiPRO_0000283915769 – 1363Spike protein S2By similarityAdd BLAST595

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi133N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi198N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi359N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi437N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi649N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi676N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi696N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi714N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi739N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi788N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi937N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi1194N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi1224N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi1234N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi1253N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi1267N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi1288N-linked (GlcNAc...) asparagine; by hostSequence analysis1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. The precursor is processed into S1 and S2 by host cell furin or furin-like protease to yield the mature S1 and S2 proteins. The cleavage site between S1 and S2 requires the optimal sequence [KR]-X-[KR]-R. Cleavage is not necessary for virus-cell fusion, and fusion probably occurs at the host plasma membrane (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei768 – 769Cleavage; by hostBy similarity2

Keywords - PTMi

Cleavage on pair of basic residues, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; each monomer consists of a S1 and a S2 subunit. The resulting peplomers protrude from the virus surface as spikes (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8V436.
SMRiQ8V436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1043 – 1086Heptad repeat 1Sequence analysisAdd BLAST44
Regioni1267 – 1297Heptad repeat 2Sequence analysisAdd BLAST31

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1043 – 1087Sequence analysisAdd BLAST45
Coiled coili1269 – 1297Sequence analysisAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1329 – 1346Cys-richAdd BLAST18

Sequence similaritiesi

Belongs to the coronaviruses spike protein family.Curated

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG0900000Z.

Family and domain databases

Gene3Di1.20.5.790. 1 hit.
InterProiView protein in InterPro
IPR002552. Corona_S2.
IPR027400. S_HR2.
IPR032500. Spike_N.
IPR018548. Spike_rcpt-bd.
IPR036326. Spike_rcpt-bd_sf.
PfamiView protein in Pfam
PF01601. Corona_S2. 1 hit.
PF16451. Spike_NTD. 1 hit.
PF09408. Spike_rec_bind. 1 hit.
SUPFAMiSSF143587. SSF143587. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8V436-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLILLISLP TAFAVIGDLK CTTVSINDVD TGVPSISTDT VDVTKGLGTY
60 70 80 90 100
YVLDRVYLNT TLLLNGYYPT SGSTYRNMAL KGTLLLSTLW FKPPFLSDFT
110 120 130 140 150
NGIFAKVKNT KVIKDGVMYS EFPAITIGST FVNTSYSVVV QPHTTILGNK
160 170 180 190 200
LQGFLEISVC QYTMCEYPNT ICNPNLGNRR VELWHWDTGV VSCLYKRNFT
210 220 230 240 250
YDVNADYLYF HFYQEGGTFY AYFTDTGVVT KFLFNVYLGT VLSHYYVMPL
260 270 280 290 300
TCNSALTLEY WVTPLTSKQY LLAFNQDGVI FNAVDCKSDF MSEIKCKTLS
310 320 330 340 350
IAPSTGVYEL NGYTVQPIAD VYRRIPNLPD CNIEAWLNDK SVPSPLNWER
360 370 380 390 400
KTFSNCNFNM SSLMSFIQAD SFTCNNIDAA KIYGMCFSSI TIDKFAIPNG
410 420 430 440 450
RKVDLQLGNL GYLQSFNYRI DTTATSCQLY YNLPAANVSV SRFNPSTWNR
460 470 480 490 500
RFGFTEQSVF KPQPAGVFTD HDVVYAQHCF KAPTNFCPCK LDGSLCVGNG
510 520 530 540 550
PGIDAGYKTS GIGTCPAGTN YLTCHNAAQC DCLCTPDPIT SKATGPYKCP
560 570 580 590 600
QTKYLVGIGE HCSGLAIKSD HCGGNPCTCQ PQAFLGWSVD SCLQGDRCNI
610 620 630 640 650
FANFILHDVN SGTTCSTDLQ KSNTDIILGV CVNYDLYGIT GQGIFVEVNA
660 670 680 690 700
TYYNSWQNLL YDSNGNLYGF RDYLTNRTFM IRSCYSGRVS AAFHANSSEP
710 720 730 740 750
ALLFRNIKCN YVFNNTLSRQ LQPINYFDSY LGCVVNADNS TSSVVQTCDL
760 770 780 790 800
TVGSGYCVDY STKRRSRRSI TTGYRFTNFE PFTVNSVNDS LEPVGGLYEI
810 820 830 840 850
QIPSEFTIGN MEEFIQTSSP KVTIDCSAFV CGDYAACKSQ LVEYGSFCDN
860 870 880 890 900
INAILTEVNE LLDTTQLQVA NSLMNGVTLS TKLKDGVNFN VDDINFSPVL
910 920 930 940 950
GCLGSDCNKV SSRSAIEDLL FSKVKLSDVG FVEAYNNCTG GAEIRDLICV
960 970 980 990 1000
QSYNGIKVLP PLLSENQISG YTLAATSASL FPPWSAAAGV PFYLNVQYRI
1010 1020 1030 1040 1050
NGIGVTMDVL SQNQKLIANA FNNALGAIQE GFDATNSALV KIQAVVNANA
1060 1070 1080 1090 1100
ETLNNLLQQL SNRFGAISSS LQEILSRLDA LEAQAQIDRL INGRLTALNA
1110 1120 1130 1140 1150
YVSQQLSDST LVKFSAAQAM EKVNECVKSQ SSRINFCGNG NHIISLVQNA
1160 1170 1180 1190 1200
PYGLYFIHFS YVPTKYVTAK VSPGLCIAGG RGIAPKSGYF VNVNNTWMFT
1210 1220 1230 1240 1250
GSGYYYPEPI TGNNVVVMST CAVNYTKAPD VMLNISTPNL PDFKEELDQW
1260 1270 1280 1290 1300
FKNQTSVAPD LSLDYINVTF LDLQDEMNRL QEAIKVLNQS YINLKDIGTY
1310 1320 1330 1340 1350
EYYVKWPWYV WLLIGFAGVA MLVLLFFICC CTGCGTSCFK KCGGCCDDYT
1360
GHQELVIKTS HED
Length:1,363
Mass (Da):150,543
Last modified:March 1, 2002 - v1
Checksum:i6824B55A588502D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF391542 Genomic RNA. Translation: AAL57308.1.

Similar proteinsi

Entry informationi

Entry nameiSPIKE_CVBLU
AccessioniPrimary (citable) accession number: Q8V436
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: March 1, 2002
Last modified: October 25, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome