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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain RVA/Monkey/United States/RRV/1980/G3P5B[3]) (RV-A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection (By similarity).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain RVA/Monkey/United States/RRV/1980/G3P5B[3]) (RV-A)
Taxonomic identifieri10969 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003678171 – 313Non-structural protein 3Add BLAST313

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8UZL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 149RNA-bindingBy similarityAdd BLAST146
Regioni150 – 206DimerizationBy similarityAdd BLAST57
Regioni170 – 234Interaction with ZC3H7BBy similarityAdd BLAST65
Regioni208 – 313Interaction with EIF4G1By similarityAdd BLAST106

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili166 – 237Sequence analysisAdd BLAST72

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.Curated

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UZL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKMESTQQM ASSIINSSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF
60 70 80 90 100
DLVMDDSGVK NNLIGKAITI DQALNGKFSS AIRNRNWMTD SRTVAKLDED
110 120 130 140 150
VNKLRIMLSS KGIDQKMRVL NACFSVKRIP GKSSSIVKCT RLMKDKLERG
160 170 180 190 200
EVEVDDSFVE EKMEVDTIDW KSRYEQLEKR FESLKHRVNE KYNHWVLKAR
210 220 230 240 250
KVNENMNSLQ NVISQQQAHI NELQMYNNKL ERDLQSKIGS VVSSIEWYLR
260 270 280 290 300
SMELSDDVKS DIEQQLNSID QLNPVNAIDD FESILRNLIS DYDRLFIMFK
310
GLLQQCNYTY TYE
Length:313
Mass (Da):36,315
Last modified:March 1, 2002 - v1
Checksum:iC07F5AC22D8F5932
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065842 Genomic RNA. Translation: AAL58536.1.
DQ391186 Genomic RNA. Translation: ABD52871.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY065842 Genomic RNA. Translation: AAL58536.1.
DQ391186 Genomic RNA. Translation: ABD52871.1.

3D structure databases

ProteinModelPortaliQ8UZL8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNSP3_ROTRH
AccessioniPrimary (citable) accession number: Q8UZL8
Secondary accession number(s): Q27PP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.