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Q8UZL8 (NSP3_ROTRH) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-structural protein 3

Short name=NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name=NS34
OrganismRotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Taxonomic identifier10969 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostMacaca mulatta (Rhesus macaque) [TaxID: 9544]

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity. Ref.3 Ref.4

Subunit structure

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Sequence similarities

Belongs to the rotavirus A NSP3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Non-structural protein 3
PRO_0000367817

Regions

Region4 – 149146RNA-binding By similarity
Region150 – 20657Dimerization By similarity
Region170 – 23465Interaction with ZC3H7B By similarity
Region208 – 313106Interaction with EIF4G1 By similarity
Coiled coil166 – 23772 Potential

Sequences

Sequence LengthMass (Da)Tools
Q8UZL8 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C07F5AC22D8F5932

FASTA31336,315
        10         20         30         40         50         60 
MLKMESTQQM ASSIINSSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF DLVMDDSGVK 

        70         80         90        100        110        120 
NNLIGKAITI DQALNGKFSS AIRNRNWMTD SRTVAKLDED VNKLRIMLSS KGIDQKMRVL 

       130        140        150        160        170        180 
NACFSVKRIP GKSSSIVKCT RLMKDKLERG EVEVDDSFVE EKMEVDTIDW KSRYEQLEKR 

       190        200        210        220        230        240 
FESLKHRVNE KYNHWVLKAR KVNENMNSLQ NVISQQQAHI NELQMYNNKL ERDLQSKIGS 

       250        260        270        280        290        300 
VVSSIEWYLR SMELSDDVKS DIEQQLNSID QLNPVNAIDD FESILRNLIS DYDRLFIMFK 

       310 
GLLQQCNYTY TYE 

« Hide

References

[1]"Rotavirus genome segment 7 (NSP3) is a determinant of extraintestinal spread in the neonatal mouse."
Mossel E.C., Ramig R.F.
J. Virol. 76:6502-6509(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Extraintestinal spread and replication of a homologous EC rotavirus strain and a heterologous rhesus rotavirus in BALB/c mice."
Fenaux M., Cuadras M.A., Feng N., Jaimes M., Greenberg H.B.
J. Virol. 80:5219-5232(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 2-213.
[3]"Rotavirus protein NSP3 shuts off host cell protein synthesis."
Padilla-Noriega L., Paniagua O., Guzman-Leon S.
Virology 298:1-7(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Rotavirus nonstructural protein NSP3 is not required for viral protein synthesis."
Montero H., Arias C.F., Lopez S.
J. Virol. 80:9031-9038(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY065842 Genomic RNA. Translation: AAL58536.1.
DQ391186 Genomic RNA. Translation: ABD52871.1.

3D structure databases

ProteinModelPortalQ8UZL8.
SMRQ8UZL8. Positions 7-163, 206-312.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMSSF69903. SSF69903. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNSP3_ROTRH
AccessionPrimary (citable) accession number: Q8UZL8
Secondary accession number(s): Q27PP2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2002
Last modified: October 16, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families