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Q8UZL8

- NSP3_ROTRH

UniProt

Q8UZL8 - NSP3_ROTRH

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Protein

Non-structural protein 3

Gene
N/A
Organism
Rotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Involved in translation of viral mRNAs and shutoff of host protein synthesis. It is unclear whether it is required for the translation of viral mRNAs as well. Functions similarly to, and competes with the cellular poly(A)-binding protein PABPC1. Binds the conserved sequence 'GACC' at the 3' end of viral mRNAs and allows circularization of viral mRNAs in translation. Interacts with ZC3H7B/RoXaN and with the eukaryotic translation initiation factor eIF4G, using the same region employed by PABP-C1. NSP3 thus displaces PABPC1 from eIF4G, inhibiting the translation of cellular poly(A) mRNAs. Also responsible for the nuclear relocalization of PABPC1 upon rotavirus infection By similarity.2 Publications

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Translation regulation

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein 3
Short name:
NSP3
Alternative name(s):
NCVP4
Non-structural RNA-binding protein 34
Short name:
NS34
OrganismiRotavirus A (strain Monkey/United States/RRV/1980 G3-P5B[3]-I2-R2-C3-M3-A9-Nx-T3-E3-H6) (RV-A)
Taxonomic identifieri10969 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiMacaca mulatta (Rhesus macaque) [TaxID: 9544]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Non-structural protein 3PRO_0000367817Add
BLAST

Interactioni

Subunit structurei

Homodimer. Interacts (via the coiled-coil region) with host ZC3H7B (via LD motif). Interacts with host EIF4G1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ8UZL8.
SMRiQ8UZL8. Positions 7-163, 206-312.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni4 – 149146RNA-binding By similarityAdd
BLAST
Regioni150 – 20657Dimerization By similarityAdd
BLAST
Regioni170 – 23465Interaction with ZC3H7B By similarityAdd
BLAST
Regioni208 – 313106Interaction with EIF4G1 By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili166 – 23772 Reviewed predictionAdd
BLAST

Sequence similaritiesi

Belongs to the rotavirus A NSP3 family.

Keywords - Domaini

Coiled coil

Family and domain databases

InterProiIPR002873. Rotavirus_NSP3.
[Graphical view]
PfamiPF01665. Rota_NSP3. 1 hit.
[Graphical view]
SUPFAMiSSF69903. SSF69903. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UZL8-1 [UniParc]FASTAAdd to Basket

« Hide

MLKMESTQQM ASSIINSSFE AAVVAATSTL ELMGIQYDYN EVYTRVKSKF    50
DLVMDDSGVK NNLIGKAITI DQALNGKFSS AIRNRNWMTD SRTVAKLDED 100
VNKLRIMLSS KGIDQKMRVL NACFSVKRIP GKSSSIVKCT RLMKDKLERG 150
EVEVDDSFVE EKMEVDTIDW KSRYEQLEKR FESLKHRVNE KYNHWVLKAR 200
KVNENMNSLQ NVISQQQAHI NELQMYNNKL ERDLQSKIGS VVSSIEWYLR 250
SMELSDDVKS DIEQQLNSID QLNPVNAIDD FESILRNLIS DYDRLFIMFK 300
GLLQQCNYTY TYE 313
Length:313
Mass (Da):36,315
Last modified:March 1, 2002 - v1
Checksum:iC07F5AC22D8F5932
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY065842 Genomic RNA. Translation: AAL58536.1.
DQ391186 Genomic RNA. Translation: ABD52871.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY065842 Genomic RNA. Translation: AAL58536.1 .
DQ391186 Genomic RNA. Translation: ABD52871.1 .

3D structure databases

ProteinModelPortali Q8UZL8.
SMRi Q8UZL8. Positions 7-163, 206-312.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR002873. Rotavirus_NSP3.
[Graphical view ]
Pfami PF01665. Rota_NSP3. 1 hit.
[Graphical view ]
SUPFAMi SSF69903. SSF69903. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Rotavirus genome segment 7 (NSP3) is a determinant of extraintestinal spread in the neonatal mouse."
    Mossel E.C., Ramig R.F.
    J. Virol. 76:6502-6509(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Extraintestinal spread and replication of a homologous EC rotavirus strain and a heterologous rhesus rotavirus in BALB/c mice."
    Fenaux M., Cuadras M.A., Feng N., Jaimes M., Greenberg H.B.
    J. Virol. 80:5219-5232(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 2-213.
  3. "Rotavirus protein NSP3 shuts off host cell protein synthesis."
    Padilla-Noriega L., Paniagua O., Guzman-Leon S.
    Virology 298:1-7(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Rotavirus nonstructural protein NSP3 is not required for viral protein synthesis."
    Montero H., Arias C.F., Lopez S.
    J. Virol. 80:9031-9038(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiNSP3_ROTRH
AccessioniPrimary (citable) accession number: Q8UZL8
Secondary accession number(s): Q27PP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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