ID   Q8UYL3_9POXV            Unreviewed;       167 AA.
AC   Q8UYL3;
DT   01-MAR-2002, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2002, sequence version 1.
DT   27-MAR-2024, entry version 131.
DE   SubName: Full=CrmE protein {ECO:0000313|EMBL:CAC83048.1};
DE   SubName: Full=TNF-alpha-receptor-like protein {ECO:0000313|EMBL:AIZ72951.1};
GN   Name=crmE {ECO:0000313|EMBL:CAC83048.1};
GN   Synonyms=B28R {ECO:0000313|EMBL:AIZ72951.1};
GN   ORFNames=List195 {ECO:0000313|EMBL:ABD52700.1}, m8259R
GN   {ECO:0000313|EMBL:AAW23648.1}, mO259R {ECO:0000313|EMBL:AAW23930.1};
OS   Vaccinia virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10245 {ECO:0000313|EMBL:CAC83048.1};
RN   [1] {ECO:0000313|EMBL:CAC83048.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=USSR {ECO:0000313|EMBL:CAC83048.1};
RX   PubMed=11878931; DOI=10.1006/viro.2001.1236;
RA   Reading P.C., Khanna A., Smith G.L.;
RT   "Vaccinia virus CrmE encodes a soluble and cell surface tumor necrosis
RT   factor receptor that contributes to virus virulence.";
RL   Virology 292:285-298(2002).
RN   [2] {ECO:0000313|Proteomes:UP000171434, ECO:0000313|Proteomes:UP000173158}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LC16m8 {ECO:0000313|EMBL:AAW23648.1}, and LC16mO
RC   {ECO:0000313|EMBL:AAW23930.1};
RX   PubMed=16140764; DOI=10.1128/JVI.79.18.11873-11891.2005;
RA   Morikawa S., Sakiyama T., Hasegawa H., Saijo M., Maeda A., Kurane I.,
RA   Maeno G., Kimura J., Hirama C., Yoshida T., Asahi-Ozaki Y., Sata T.,
RA   Kurata T., Kojima A.;
RT   "An attenuated LC16m8 smallpox vaccine: analysis of full-genome sequence
RT   and induction of immune protection.";
RL   J. Virol. 79:11873-11891(2005).
RN   [3] {ECO:0000313|EMBL:ABD52700.1, ECO:0000313|Proteomes:UP000180811}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Lister {ECO:0000313|EMBL:ABD52700.1};
RX   PubMed=17554021; DOI=10.1099/vir.0.82708-0;
RA   Garcel A., Crance J.M., Drillien R., Garin D., Favier A.L.;
RT   "Genomic sequence of a clonal isolate of the vaccinia virus Lister strain
RT   employed for smallpox vaccination in France and its comparison to other
RT   orthopoxviruses.";
RL   J. Gen. Virol. 88:1906-1916(2007).
RN   [4] {ECO:0007829|PDB:2UWI}
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 22-153, AND DISULFIDE BONDS.
RX   PubMed=17681535; DOI=10.1016/J.JMB.2007.06.082;
RA   Graham S.C., Bahar M.W., Abrescia N.G., Smith G.L., Stuart D.I.,
RA   Grimes J.M.;
RT   "Structure of CrmE, a virus-encoded tumour necrosis factor receptor.";
RL   J. Mol. Biol. 372:660-671(2007).
RN   [5] {ECO:0000313|EMBL:AIZ72951.1, ECO:0000313|Proteomes:UP000136774}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L-IVP {ECO:0000313|EMBL:AIZ72951.1};
RA   Kochneva G.V., Babkina I.N., Lupan T.A., Grazhdantseva A.A., Yudin P.V.,
RA   Sivolobova G.F., Shvalov A.N., Popov E.G., Babkin I.V., Netesov S.V.,
RA   Chumakov P.M.;
RT   "Apoptin enhances the oncolytic activity of vaccinia virus in vitro.";
RL   Mol. Biol. 47:733-742(2013).
RN   [6] {ECO:0000313|EMBL:AIZ72951.1, ECO:0000313|Proteomes:UP000136774}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L-IVP {ECO:0000313|EMBL:AIZ72951.1};
RX   PubMed=25358248;
RA   Kochneva G., Zonov E., Grazhdantseva A., Yunusova A., Sibolobova G.,
RA   Popov E., Taranov O., Netesov S., Chumakov P., Ryabchikova E.;
RT   "Apoptin enhances the oncolytic properties of vaccinia virus and modifies
RT   mechanisms of tumor regression.";
RL   Oncotarget 5:11269-11282(2014).
RN   [7] {ECO:0000313|EMBL:AIZ72951.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=L-IVP {ECO:0000313|EMBL:AIZ72951.1};
RA   Lo Raquel;
RL   Submitted (DEC-2014) to the EMBL/GenBank/DDBJ databases.
RN   [8] {ECO:0000313|EMBL:QEG58999.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KVAC103 {ECO:0000313|EMBL:QEG58999.1};
RA   Kim Y.-J.;
RT   "Development of an attenuated Small pox vaccine candidate, KVAC103 vaccinia
RT   virus.";
RL   Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- INTERACTION:
CC       Q8UYL3; P01375: TNF; Xeno; NbExp=3; IntAct=EBI-7539950, EBI-359977;
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|PROSITE-ProRule:PRU00206}.
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DR   EMBL; AY678275; AAW23648.1; -; Genomic_DNA.
DR   EMBL; AY678277; AAW23930.1; -; Genomic_DNA.
DR   EMBL; DQ121394; ABD52700.1; -; Genomic_DNA.
DR   EMBL; KP233807; AIZ72951.1; -; Genomic_DNA.
DR   EMBL; AJ315962; CAC83048.1; -; Genomic_DNA.
DR   EMBL; MK336431; QEG58999.1; -; Genomic_DNA.
DR   PDB; 2UWI; X-ray; 2.00 A; A/B=22-153.
DR   PDBsum; 2UWI; -.
DR   SMR; Q8UYL3; -.
DR   IntAct; Q8UYL3; 1.
DR   MINT; Q8UYL3; -.
DR   Proteomes; UP000136774; Genome.
DR   Proteomes; UP000171434; Genome.
DR   Proteomes; UP000173158; Genome.
DR   Proteomes; UP000180811; Genome.
DR   Proteomes; UP000439479; Genome.
DR   CDD; cd15839; TNFRSF_viral; 1.
DR   Gene3D; 2.10.50.10; Tumor Necrosis Factor Receptor, subunit A, domain 2; 2.
DR   InterPro; IPR001368; TNFR/NGFR_Cys_rich_reg.
DR   InterPro; IPR034059; TNFRSF_N_viral.
DR   PANTHER; PTHR47386; TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 1B; 1.
DR   PANTHER; PTHR47386:SF1; TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 1B; 1.
DR   Pfam; PF00020; TNFR_c6; 1.
DR   SMART; SM00208; TNFR; 3.
DR   SUPFAM; SSF57586; TNF receptor-like; 2.
DR   PROSITE; PS00652; TNFR_NGFR_1; 2.
DR   PROSITE; PS50050; TNFR_NGFR_2; 2.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:2UWI};
KW   Disulfide bond {ECO:0000256|PROSITE-ProRule:PRU00206};
KW   Receptor {ECO:0000313|EMBL:AIZ72951.1}.
FT   DOMAIN          21..58
FT                   /note="TNFR-Cys"
FT                   /evidence="ECO:0000259|PROSITE:PS50050"
FT   REPEAT          21..58
FT                   /note="TNFR-Cys"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00206"
FT   DOMAIN          60..101
FT                   /note="TNFR-Cys"
FT                   /evidence="ECO:0000259|PROSITE:PS50050"
FT   REPEAT          60..101
FT                   /note="TNFR-Cys"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00206"
FT   DISULFID        22..36
FT                   /evidence="ECO:0007829|PDB:2UWI"
FT   DISULFID        37..50
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00206,
FT                   ECO:0007829|PDB:2UWI"
FT   DISULFID        40..58
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00206,
FT                   ECO:0007829|PDB:2UWI"
FT   DISULFID        61..76
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00206,
FT                   ECO:0007829|PDB:2UWI"
FT   DISULFID        79..93
FT                   /evidence="ECO:0007829|PDB:2UWI"
FT   DISULFID        83..101
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00206,
FT                   ECO:0007829|PDB:2UWI"
FT   DISULFID        103..121
FT                   /evidence="ECO:0007829|PDB:2UWI"
FT   DISULFID        109..118
FT                   /evidence="ECO:0007829|PDB:2UWI"
FT   DISULFID        127..144
FT                   /evidence="ECO:0007829|PDB:2UWI"
SQ   SEQUENCE   167 AA;  18510 MW;  3B4A0D4A27FB797D CRC64;
     MTKVIIILGF LIINTNSLSM KCEQGVSYYN SQELKCCKLC KPGTYSDHRC DKYSDTICGH
     CPSDTFTSIY NRSPWCHSCR GPCGTNRVEV TPCTPTTNRI CHCDSNSYCL LKASDGNCVT
     CAPKTKCGRG YGKKGEDEMG NTICKKCRKG TYSDIVSDSD QCKPMTR
//