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Protein

Proteasome subunit beta type-9

Gene

psmb9

Organism
Oryzias latipes (Medaka fish) (Japanese ricefish)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides (By similarity).By similarity

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei18 – 192Cleavage; by autolysisBy similarity
Active sitei19 – 191NucleophileBy similarity

GO - Molecular functioni

  1. threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. immune system process Source: UniProtKB-KW
  2. proteolysis involved in cellular protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Keywords - Biological processi

Immunity

Protein family/group databases

MEROPSiT01.013.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-9 (EC:3.4.25.1)
Alternative name(s):
Low molecular mass protein 2
Gene namesi
Name:psmb9
Synonyms:lmp2
OrganismiOryzias latipes (Medaka fish) (Japanese ricefish)
Taxonomic identifieri8090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataOvalentariaAtherinomorphaeBeloniformesAdrianichthyidaeOryziinaeOryzias
ProteomesiUP000001038: Unplaced

Subcellular locationi

Cytoplasm PROSITE-ProRule annotation. Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
  3. proteasome core complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1818Removed in mature formBy similarityPRO_0000026633Add
BLAST
Chaini19 – 217199Proteasome subunit beta type-9PRO_0000026634Add
BLAST

Post-translational modificationi

Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity.By similarity

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Component of the immunoproteasome, where it displaces the equivalent houskeeping subunit PSMB6 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8UW64.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091079.
HOVERGENiHBG000123.
InParanoidiQ8UW64.
KOiK02741.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8UW64-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGEEAEPQW ISEEVKTGTT IIAIEFNGGV VLGSDSRVSA GDSVVNRVMN
60 70 80 90 100
KLSPLHDKIY CALSGSAADA QTIAEMVNYQ LDVHSLEIDE DPQVRSAATL
110 120 130 140 150
VKNISYKYKE ELSAHLIVAG WDRRDGGQVF ATLGGLLTRQ PFAIGGSGSS
160 170 180 190 200
YVYGFVDAEY RRGMTKEECQ KFVVNTLALA MNRDGSSGGV AYIVTIDEHS
210
TDEKVILGND LPTFFDQ
Length:217
Mass (Da):23,547
Last modified:March 1, 2002 - v1
Checksum:iA9AB1C04E57EB4C9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti5 – 51Missing in strain: HD-RR.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000027 Genomic DNA. Translation: BAB83845.1.
AB073378 Genomic DNA. Translation: BAB84548.1.
RefSeqiNP_001265756.1. NM_001278827.1.
UniGeneiOla.82.

Genome annotation databases

GeneIDi100049434.
KEGGiola:100049434.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000027 Genomic DNA. Translation: BAB83845.1.
AB073378 Genomic DNA. Translation: BAB84548.1.
RefSeqiNP_001265756.1. NM_001278827.1.
UniGeneiOla.82.

3D structure databases

ProteinModelPortaliQ8UW64.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiT01.013.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100049434.
KEGGiola:100049434.

Organism-specific databases

CTDi5698.

Phylogenomic databases

eggNOGiCOG0638.
HOGENOMiHOG000091079.
HOVERGENiHBG000123.
InParanoidiQ8UW64.
KOiK02741.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the MHC class I genomic region of a teleost, the medaka (Oryzias latipes)."
    Matsuo M.Y., Asakawa S., Shimizu N., Kimura H., Nonaka M.
    Immunogenetics 53:930-940(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Hd-rR and HNI.

Entry informationi

Entry nameiPSB9_ORYLA
AccessioniPrimary (citable) accession number: Q8UW64
Secondary accession number(s): Q8UWC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.