Reviewed, UniProtKB/Swiss-Prot Q8UW08 (PA20_LAPHA)

Names and origin

Protein names	Recommended name:     Phospholipase A2     EC=3.1.1.4 Alternative name(s):     Phosphatidylcholine 2-acylhydrolase
Organism	Lapemis hardwickii (Hardwick's sea snake)
Taxonomic identifier	8781 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Hydrophiidae › Lapemis

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted By similarity.
Tissue specificity	Expressed by the venom gland Probable.
Sequence similarities	Belongs to the phospholipase A2 family. Group I subfamily.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 27	6	By similarity		PRO_0000022876
	Chain	28 – 146	119	Phospholipase A2		PRO_0000022877
Sites
	Active site	75	1	By similarity
	Active site	120	1	By similarity
	Metal binding	55	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	57	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	59	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	76	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	38 ↔ 98		By similarity
	Disulfide bond	54 ↔ 145		By similarity
	Disulfide bond	56 ↔ 72		By similarity
	Disulfide bond	71 ↔ 126		By similarity
	Disulfide bond	78 ↔ 119		By similarity
	Disulfide bond	87 ↔ 112		By similarity
	Disulfide bond	105 ↔ 117		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UW08-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: FDD58BF5BD8E38C1	FASTA	146	16,114
	10 20 30 40 50 60 MYPAHLLVLL AVCVSLLGAA SIPPLPLNLV QFSYVITCAN HGRRSSLDYA DYGCYCGAGG 70 80 90 100 110 120 SGTPVDELDR CCQIHDDCYG EAEKQGCYPK MLIYDYYCGS DGPYCRNVKK KCNRMVCDCD 130 140 VAAAKCFARN AYNNANYNID TNKRCK

References

[1]	"A novel PLA2-like cDNA clone from sea snake venom." Wei J., Yang W., Zhao G., Zhong X., Xu A. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF205378 mRNA. Translation: AAL54920.1.
3D structure databases
SMR	Q8UW08. Positions 28-146.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q8UW08.
Enzyme and pathway databases
BRENDA	3.1.1.4. 274608.
Family and domain databases
InterPro	IPR016090. Phospholipase_A2. IPR013090. Phospholipase_A2_AS. IPR001211. Phospholipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA20_LAPHA

Accession

Primary (citable) accession number: Q8UW08

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	March 1, 2002
Last modified:	November 24, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)