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Q8UVX0 (PIWL1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Piwi-like protein 1
Gene names
Name:piwil1
Synonyms:ziwi
OrganismDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length858 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a central role during gametogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Has a strong preference for piRNAs with a uridine nucleotide at their 5'-end and binds piRNAs in an opposite direction compared to piwil2/zili. Participates in a piRNA amplification loop with piwil2/zili. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Ref.1 Ref.3 Ref.4

Subcellular location

Cytoplasm. Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Ref.3

Tissue specificity

Expressed exclusively in the adult gonads; expression in the ovary weaker than in the testis (at protein level). During neurogenesis and organogenesis, expression is detected in CNS (midbrain and eye) and fin buds. Starting from 24 hours post-fertilization, expression is found in the genital ridge. Ref.1 Ref.3

Developmental stage

Initially detected during embryonic segmentation which persists for at least 4 weeks post hatching. Ref.1

Domain

The PAZ domain specifically recognizes binds the 2'-O-methylated 3'-end of piRNAs By similarity.

Post-translational modification

Methylated on arginine residues; required for the interaction with Tudor domain-containing protein and subsequent localization to the meiotic nuage, also named P granule By similarity.

Disruption phenotype

Progressive loss of germ cells due to apoptosis during larval development. Ref.3

Sequence similarities

Belongs to the argonaute family. Piwi subfamily.

Contains 1 PAZ domain.

Contains 1 Piwi domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8UVX0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8UVX0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     350-373: DITDGNQVLLVSHVKRLGPSGRPP → VIIRLLFFVIRCSNLKFLYLEIVN
     374-858: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 858858Piwi-like protein 1
PRO_0000194066

Regions

Domain272 – 388117PAZ
Domain552 – 844293Piwi
Region314 – 3163Required for binding 2'-O-methylated 3'-end of piRNAs By similarity
Region537 – 674138RNA-binding By similarity

Sites

Site3781Required for binding 2'-O-methylated 3'-end of piRNAs By similarity

Natural variations

Alternative sequence350 – 37324DITDG…SGRPP → VIIRLLFFVIRCSNLKFLYL EIVN in isoform 2.
VSP_050727
Alternative sequence374 – 858485Missing in isoform 2.
VSP_050728

Experimental info

Mutagenesis6871N → K in hu2410; induces germ cells apoptosis. Ref.3
Mutagenesis6941I → N in fh222; induces germ cells apoptosis. Ref.3
Sequence conflict3221W → R in AAH49495. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 6A12F2E511465777

FASTA85897,451
        10         20         30         40         50         60 
MTGRARARSR GRGRGQEPAA PGAQPPVSQE AAKPVVSTPS EGQLVGRGRQ KPAPGAMSEE 

        70         80         90        100        110        120 
AMLQISAGFQ QVKIGERGGR RRDFHDSGVH TRQLMEHVKE SKTGVSGTAI ELRANFMRLL 

       130        140        150        160        170        180 
SRPMWALYQY HVDYKPPMES RRLRSALLFQ HEETLGKAHT FDGAILFLPN KLRNAETVLC 

       190        200        210        220        230        240 
SETRNGEKVE ITVTLTNELP PSSPVCLQFY NILFRRILRI LNMQQIGRHY YNPDDPFNIP 

       250        260        270        280        290        300 
QHRLTIWPGF MTTILQYESS IMLCSDVSHK VLRSETVLDF MYSLRQQCGD QRFPEACTKE 

       310        320        330        340        350        360 
LVGLIILTKY NNKTYRIDDI AWDHTPNNTF KKGDTEISFK NYFKSQYGLD ITDGNQVLLV 

       370        380        390        400        410        420 
SHVKRLGPSG RPPPGPAMLV PEFCYLTGLT DKMRADFNIM KDLASHTRLS PEQREGRINR 

       430        440        450        460        470        480 
LISNINRNGD VQNELTTWGL SFENKLLSLN GRVLPSERII QGGRAFEYNP WTADWSKEMR 

       490        500        510        520        530        540 
GLPLISCMSL DNWLMFYTRR NADVAQSLLQ TLNKVSGPMG IRMQRAVMIE YEDRQESLLR 

       550        560        570        580        590        600 
ALQQNVARET QMVVVILPTN RKDKYDCVKK YLCVDCPTPS QCVVSRTISK PQALMTVATK 

       610        620        630        640        650        660 
IALQMNCKMG GELWSVEIPL RQLMIVGIDC YHDTAAGKRS IGAMVASLNQ GMSRWFSKCV 

       670        680        690        700        710        720 
LQNRGQEIID ALKGSLQGAL KAYLKYNNSL PSRIIVYRDG VGDGMLQSVV DYEVPQIMQS 

       730        740        750        760        770        780 
IKTMGQDYEP KLSVVVVKKR ISSRFFARID GKIANPPPGT VIDTEVTRPE WYDFFIVSQA 

       790        800        810        820        830        840 
VRFGCVAPTH YNVVFDNSGL KPDHMQRLTY KLCHMYYNWQ GIVRVPAPCQ YAHKLAFLVG 

       850 
QSIHKEPNMN LDDFLYYL

« Hide

Isoform 2 [UniParc].

Checksum: F7D6AF6662DCA600
Show »

FASTA37342,687

References

« Hide 'large scale' references
[1]"Ziwi, the zebrafish homologue of the Drosophila piwi: co-localization with vasa at the embryonic genital ridge and gonad-specific expression in the adults."
Tan C.H., Lee T.C., Weeraratne S.D., Korzh V., Lim T.M., Gong Z.
Mech. Dev. 119:S221-S224(2002) [PubMed: 14516689] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Gonad.
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: AB.
[3]"A role for Piwi and piRNAs in germ cell maintenance and transposon silencing in Zebrafish."
Houwing S., Kamminga L.M., Berezikov E., Cronembold D., Girard A., van den Elst H., Filippov D.V., Blaser H., Raz E., Moens C.B., Plasterk R.H.A., Hannon G.J., Draper B.W., Ketting R.F.
Cell 129:69-82(2007) [PubMed: 17418787] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, RNA-BINDING, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASN-687 AND ILE-694.
[4]"Zili is required for germ cell differentiation and meiosis in zebrafish."
Houwing S., Berezikov E., Ketting R.F.
EMBO J. 27:2702-2711(2008) [PubMed: 18833190] [Abstract]
Cited for: FUNCTION, RNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF336369 mRNA. Translation: AAL57170.1.
BC049495 mRNA. Translation: AAH49495.1.
IPIIPI00483910.
IPI00501372.
RefSeqNP_899181.1. NM_183338.1.
UniGeneDr.83448.

3D structure databases

ProteinModelPortalQ8UVX0.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8UVX0.

Proteomic databases

PRIDEQ8UVX0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000061115; ENSDARP00000061114; ENSDARG00000041699.
ENSDART00000138019; ENSDARP00000121147; ENSDARG00000041699.
ENSDART00000145076; ENSDARP00000121108; ENSDARG00000041699.
GeneID368200.
KEGGdre:368200.

Organism-specific databases

CTD9271.
ZFINZDB-GENE-030813-2. piwil1.

Phylogenomic databases

eggNOGfiNOG13841.
GeneTreeENSGT00550000074185.
HOVERGENHBG049411.
InParanoidQ8UVX0.
OMALTIWPGF.
OrthoDBEOG47SSD7.
PhylomeDBQ8UVX0.

Gene expression databases

BgeeQ8UVX0.

Family and domain databases

InterProIPR003100. PAZ.
IPR003165. Piwi.
IPR012337. RNaseH-like_dom.
[Graphical view]
KOK02156.
PfamPF02170. PAZ. 1 hit.
PF02171. Piwi. 1 hit.
[Graphical view]
SMARTSM00949. PAZ. 1 hit.
SM00950. Piwi. 1 hit.
[Graphical view]
SUPFAMSSF101690. PAZ. 1 hit.
SSF53098. RNaseH_fold. 1 hit.
PROSITEPS50821. PAZ. 1 hit.
PS50822. PIWI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePIWL1_DANRE
AccessionPrimary (citable) accession number: Q8UVX0
Secondary accession number(s): Q7ZWB5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: March 1, 2002
Last modified: January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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