Q8UN02 (GAG80_MLVMN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycosylated Gag polyprotein
Short name=Pr80gag

Alternative name(s):
Glyco-gag
gp80gag

Cleaved into the following 2 chains:

1. Nextended-MA-p12
2. CA-NC

Gene names

Name:

gag

Organism

Moloney murine leukemia virus (strain neuropathogenic variant ts1-92b) (MoMLV)

Taxonomic identifier

882209 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Murine leukemia virus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	626 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Seems to be important for efficient replication in vivo. Ref.3
Subcellular location	Nextended-MA-p12: Host cell membrane; Single-pass type II membrane protein Potential. CA-NC: Secreted Potential.
Post-translational modification	May be cleaved within the capsid domain Potential.
Sequence similarities	Contains 1 CCHC-type zinc finger.

Ontologies

Keywords
Cellular component	Host cell membrane Host membrane Membrane Secreted
Coding sequence diversity	Alternative initiation
Domain	Coiled coil Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
PTM	Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	virion assembly Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral capsid Inferred from electronic annotation. Source: InterPro
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Pr80gag (identifier: Q8UN02-1)

Also known as: GlycoGag;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by an upstream CUG initiation codon.

Isoform Pr65gag (identifier: P03332-1)

The sequence of this isoform can be found in the external entry P03332.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 626	626	Glycosylated Gag polyprotein		PRO_0000390850
	Chain	1 – ?		Nextended-MA-p12 Potential		PRO_5000061808
	Chain	? – 626		CA-NC		PRO_0000390851
Regions
	Transmembrane	64 – 86	23	Helical; Potential
	Zinc finger	590 – 607	18	CCHC-type
	Coiled coil	526 – 566	41	Potential
	Motif	199 – 202	4	PTAP/PSAP motif
	Motif	250 – 253	4	PPXY motif
	Motif	383 – 385	3	Cell attachment site Potential
	Compositional bias	159 – 281	123	Pro-rich
Amino acid modifications
	Modified residue	280	1	Phosphoserine; by host By similarity
	Glycosylation	60	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	113	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	505	1	N-linked (GlcNAc...); by host Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform Pr80gag (GlycoGag) [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 447798AB667622A6	FASTA	626	70,503
	10 20 30 40 50 60 MGDVPGTSGA VFVARPESKN PDRFGLFGAP PLEEGYVVLV GDENLKQFPP PSEFLLSVWN 70 80 90 100 110 120 RSRAARLVCC SIVLCCLCLT VFLYLSENMG QTVTTPLSLT LDHWKDVERI AHNQSVDVKK 130 140 150 160 170 180 RRWVTFCSAE WPTFNVGWPR DGTFNRDLIT QVKIKVFSPG PHGHPDQVPY IVTWEALAFD 190 200 210 220 230 240 PPPWAKPFVH PKPPPPLPPS APSLPLEPPL STPSRSSLYP ALTPSLGAKP KPQVLSDSEG 250 260 270 280 290 300 PLIDLLTEDP PPYRDPRPPP SDGDGNSGEA TPAGEAPDPS PMASRLRGRR EPPVADSTTS 310 320 330 340 350 360 QAFPLRTGGN GQLQYWPFSS SDLYNWKNNN PSFSEDPGKL TALIESVLIT HQPTWDDCQQ 370 380 390 400 410 420 LLGTLLTGEE KQRVLLEARK AVRGDDGRPT QLPNEVDAAF PLERPDWEYT TQAGRNHLVQ 430 440 450 460 470 480 YRQLLLAGLQ NAGRSPTNLA KVKGITQGPN ESPSAFLERL KEAYRRYTPY DPEDPGQETN 490 500 510 520 530 540 VAMSFIWQSA PDIGRKLERL EDLKNKTLGD LVREAERIFN KRETPEEREE RIRRETEEKE 550 560 570 580 590 600 ERRRTEDEQK EKERDRRRHR EMSKLLATVV SGQRQDRQGG ERRRSQLDRD QCAYCKEKGH 610 620 WAKDCPKKPR GPRGPRPQTS LLTLDD
	Isoform Pr65gag [UniParc]. See P03332.		–	–

References

[1]	"Sequence analysis of a neuropathogenic variant of Moloney murine leukemia virus ts1: evidence for recombination." Szurek P.F., Vann J.M., Brooks B.R. Virus Genes 25:343-344(2002) [PubMed: 12881645] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"CUG initiation codon used for the synthesis of a cell surface antigen coded by the murine leukemia virus." Prats A.C., De Billy G., Wang P., Darlix J.L. J. Mol. Biol. 205:363-372(1989) [PubMed: 2538626] [Abstract] Cited for: ALTERNATIVE INITIATION.
[3]	"Mutation in the glycosylated gag protein of murine leukemia virus results in reduced in vivo infectivity and a novel defect in viral budding or release." Low A., Datta S., Kuznetsov Y., Jahid S., Kothari N., McPherson A., Fan H. J. Virol. 81:3685-3692(2007) [PubMed: 17267509] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF462057 Genomic DNA. Translation: AAL69908.1.
PIR	S02769.
3D structure databases
HSSP	HSSP built from PDB template 1A6B based on UniProtKB P03332.
ProteinModelPortal	Q8UN02.
SMR	Q8UN02. Positions 90-186, 304-435, 440-470, 580-619.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000840. G_retro_matrix_N. IPR002079. Gag_p12. IPR003036. Gag_p30. IPR008919. Retrov_capsid_N. IPR010999. Retrovr_matrix_N. IPR013084. Znf_CCH_retrovir. IPR001878. Znf_CCHC. [Graphical view]
Gene3D	G3DSA:1.10.150.180. G_retro_matrix_N. 1 hit. G3DSA:4.10.60.10. Znf_CCH_retrovir. 1 hit.
Pfam	PF01140. Gag_MA. 1 hit. PF01141. Gag_p12. 1 hit. PF02093. Gag_p30. 1 hit. PF00098. zf-CCHC. 1 hit. [Graphical view]
SMART	SM00343. ZnF_C2HC. 1 hit. [Graphical view]
SUPFAM	SSF47943. Retrov_capsid_N. 1 hit. SSF47836. Retrovir_matrix. 1 hit.
PROSITE	PS50158. ZF_CCHC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAG80_MLVMN

Accession

Primary (citable) accession number: Q8UN02

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	March 1, 2002
Last modified:	July 27, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program