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Q8UN02

- GAG80_MLVMN

UniProt

Q8UN02 - GAG80_MLVMN

Protein

Glycosylated Gag polyprotein

Gene

gag

Organism
Moloney murine leukemia virus (strain neuropathogenic variant ts1-92b) (MoMLV)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Seems to be important for efficient replication in vivo.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri590 – 60718CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. structural molecule activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. virion assembly Source: InterPro

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycosylated Gag polyprotein
    Short name:
    Pr80gag
    Alternative name(s):
    Glyco-gag
    gp80gag
    Cleaved into the following 2 chains:
    Gene namesi
    Name:gag
    OrganismiMoloney murine leukemia virus (strain neuropathogenic variant ts1-92b) (MoMLV)
    Taxonomic identifieri882209 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral capsid Source: InterPro

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 626CA-NCPRO_0000390851
    Chaini1 – 626626Glycosylated Gag polyproteinPRO_0000390850Add
    BLAST
    Chaini1 – ?Nextended-MA-p12Sequence AnalysisPRO_5000061808

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi60 – 601N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi113 – 1131N-linked (GlcNAc...); by hostSequence Analysis
    Modified residuei280 – 2801Phosphoserine; by hostBy similarity
    Glycosylationi505 – 5051N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    May be cleaved within the capsid domain.Curated

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ8UN02.
    SMRiQ8UN02. Positions 90-186, 304-435, 440-470, 580-619.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei64 – 8623HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili526 – 56641Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi199 – 2024PTAP/PSAP motif
    Motifi250 – 2534PPXY motif
    Motifi383 – 3853Cell attachment siteSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi159 – 281123Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri590 – 60718CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix, Zinc-finger

    Family and domain databases

    Gene3Di1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProiIPR000840. G_retro_matrix_N.
    IPR002079. Gag_p12.
    IPR003036. Gag_P30.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF01140. Gag_MA. 1 hit.
    PF01141. Gag_p12. 1 hit.
    PF02093. Gag_p30. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view]
    SMARTiSM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    PROSITEiPS50158. ZF_CCHC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform Pr80gag (identifier: Q8UN02-1) [UniParc]FASTAAdd to Basket

    Also known as: GlycoGag

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGDVPGTSGA VFVARPESKN PDRFGLFGAP PLEEGYVVLV GDENLKQFPP    50
    PSEFLLSVWN RSRAARLVCC SIVLCCLCLT VFLYLSENMG QTVTTPLSLT 100
    LDHWKDVERI AHNQSVDVKK RRWVTFCSAE WPTFNVGWPR DGTFNRDLIT 150
    QVKIKVFSPG PHGHPDQVPY IVTWEALAFD PPPWAKPFVH PKPPPPLPPS 200
    APSLPLEPPL STPSRSSLYP ALTPSLGAKP KPQVLSDSEG PLIDLLTEDP 250
    PPYRDPRPPP SDGDGNSGEA TPAGEAPDPS PMASRLRGRR EPPVADSTTS 300
    QAFPLRTGGN GQLQYWPFSS SDLYNWKNNN PSFSEDPGKL TALIESVLIT 350
    HQPTWDDCQQ LLGTLLTGEE KQRVLLEARK AVRGDDGRPT QLPNEVDAAF 400
    PLERPDWEYT TQAGRNHLVQ YRQLLLAGLQ NAGRSPTNLA KVKGITQGPN 450
    ESPSAFLERL KEAYRRYTPY DPEDPGQETN VAMSFIWQSA PDIGRKLERL 500
    EDLKNKTLGD LVREAERIFN KRETPEEREE RIRRETEEKE ERRRTEDEQK 550
    EKERDRRRHR EMSKLLATVV SGQRQDRQGG ERRRSQLDRD QCAYCKEKGH 600
    WAKDCPKKPR GPRGPRPQTS LLTLDD 626

    Note: Produced by an upstream CUG initiation codon.

    Length:626
    Mass (Da):70,503
    Last modified:March 1, 2002 - v1
    Checksum:i447798AB667622A6
    GO
    Isoform Pr65gag (identifier: P03332-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P03332.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:538
    Mass (Da):60,858
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF462057 Genomic DNA. Translation: AAL69908.1.
    PIRiS02769.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF462057 Genomic DNA. Translation: AAL69908.1 .
    PIRi S02769.

    3D structure databases

    ProteinModelPortali Q8UN02.
    SMRi Q8UN02. Positions 90-186, 304-435, 440-470, 580-619.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProi IPR000840. G_retro_matrix_N.
    IPR002079. Gag_p12.
    IPR003036. Gag_P30.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF01140. Gag_MA. 1 hit.
    PF01141. Gag_p12. 1 hit.
    PF02093. Gag_p30. 1 hit.
    PF00098. zf-CCHC. 1 hit.
    [Graphical view ]
    SMARTi SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    PROSITEi PS50158. ZF_CCHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of a neuropathogenic variant of Moloney murine leukemia virus ts1: evidence for recombination."
      Szurek P.F., Vann J.M., Brooks B.R.
      Virus Genes 25:343-344(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "CUG initiation codon used for the synthesis of a cell surface antigen coded by the murine leukemia virus."
      Prats A.C., De Billy G., Wang P., Darlix J.L.
      J. Mol. Biol. 205:363-372(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE INITIATION.
    3. "Mutation in the glycosylated gag protein of murine leukemia virus results in reduced in vivo infectivity and a novel defect in viral budding or release."
      Low A., Datta S., Kuznetsov Y., Jahid S., Kothari N., McPherson A., Fan H.
      J. Virol. 81:3685-3692(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiGAG80_MLVMN
    AccessioniPrimary (citable) accession number: Q8UN02
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 19, 2010
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3