Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8UN02 (GAG80_MLVMN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycosylated Gag polyprotein

Short name=Pr80gag
Alternative name(s):
Glyco-gag
gp80gag

Cleaved into the following 2 chains:

  1. Nextended-MA-p12
  2. CA-NC
Gene names
Name:gag
OrganismMoloney murine leukemia virus (strain neuropathogenic variant ts1-92b) (MoMLV)
Taxonomic identifier882209 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length626 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Seems to be important for efficient replication in vivo. Ref.3

Subcellular location

Nextended-MA-p12: Host cell membrane; Single-pass type II membrane protein Potential.

CA-NC: Secreted Potential.

Post-translational modification

May be cleaved within the capsid domain Potential.

Sequence similarities

Contains 1 CCHC-type zinc finger.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Pr80gag (identifier: Q8UN02-1)

Also known as: GlycoGag;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by an upstream CUG initiation codon.
Isoform Pr65gag (identifier: P03332-1)

The sequence of this isoform can be found in the external entry P03332.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 626626Glycosylated Gag polyprotein
PRO_0000390850
Chain1 – ?Nextended-MA-p12 PotentialPRO_5000061808
Chain? – 626CA-NCPRO_0000390851

Regions

Transmembrane64 – 8623Helical; Potential
Zinc finger590 – 60718CCHC-type
Coiled coil526 – 56641 Potential
Motif199 – 2024PTAP/PSAP motif
Motif250 – 2534PPXY motif
Motif383 – 3853Cell attachment site Potential
Compositional bias159 – 281123Pro-rich

Amino acid modifications

Modified residue2801Phosphoserine; by host By similarity
Glycosylation601N-linked (GlcNAc...); by host Potential
Glycosylation1131N-linked (GlcNAc...); by host Potential
Glycosylation5051N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
Isoform Pr80gag (GlycoGag) [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 447798AB667622A6

FASTA62670,503
        10         20         30         40         50         60 
MGDVPGTSGA VFVARPESKN PDRFGLFGAP PLEEGYVVLV GDENLKQFPP PSEFLLSVWN 

        70         80         90        100        110        120 
RSRAARLVCC SIVLCCLCLT VFLYLSENMG QTVTTPLSLT LDHWKDVERI AHNQSVDVKK 

       130        140        150        160        170        180 
RRWVTFCSAE WPTFNVGWPR DGTFNRDLIT QVKIKVFSPG PHGHPDQVPY IVTWEALAFD 

       190        200        210        220        230        240 
PPPWAKPFVH PKPPPPLPPS APSLPLEPPL STPSRSSLYP ALTPSLGAKP KPQVLSDSEG 

       250        260        270        280        290        300 
PLIDLLTEDP PPYRDPRPPP SDGDGNSGEA TPAGEAPDPS PMASRLRGRR EPPVADSTTS 

       310        320        330        340        350        360 
QAFPLRTGGN GQLQYWPFSS SDLYNWKNNN PSFSEDPGKL TALIESVLIT HQPTWDDCQQ 

       370        380        390        400        410        420 
LLGTLLTGEE KQRVLLEARK AVRGDDGRPT QLPNEVDAAF PLERPDWEYT TQAGRNHLVQ 

       430        440        450        460        470        480 
YRQLLLAGLQ NAGRSPTNLA KVKGITQGPN ESPSAFLERL KEAYRRYTPY DPEDPGQETN 

       490        500        510        520        530        540 
VAMSFIWQSA PDIGRKLERL EDLKNKTLGD LVREAERIFN KRETPEEREE RIRRETEEKE 

       550        560        570        580        590        600 
ERRRTEDEQK EKERDRRRHR EMSKLLATVV SGQRQDRQGG ERRRSQLDRD QCAYCKEKGH 

       610        620 
WAKDCPKKPR GPRGPRPQTS LLTLDD 

« Hide

Isoform Pr65gag [UniParc].

See P03332.

References

[1]"Sequence analysis of a neuropathogenic variant of Moloney murine leukemia virus ts1: evidence for recombination."
Szurek P.F., Vann J.M., Brooks B.R.
Virus Genes 25:343-344(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"CUG initiation codon used for the synthesis of a cell surface antigen coded by the murine leukemia virus."
Prats A.C., De Billy G., Wang P., Darlix J.L.
J. Mol. Biol. 205:363-372(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE INITIATION.
[3]"Mutation in the glycosylated gag protein of murine leukemia virus results in reduced in vivo infectivity and a novel defect in viral budding or release."
Low A., Datta S., Kuznetsov Y., Jahid S., Kothari N., McPherson A., Fan H.
J. Virol. 81:3685-3692(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF462057 Genomic DNA. Translation: AAL69908.1.
PIRS02769.

3D structure databases

ProteinModelPortalQ8UN02.
SMRQ8UN02. Positions 90-186, 304-435, 440-470, 580-619.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAG80_MLVMN
AccessionPrimary (citable) accession number: Q8UN02
Entry history
Integrated into UniProtKB/Swiss-Prot: January 19, 2010
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families