Q8UKK2 (DNE22_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase 2
EC=2.7.7.7

Gene names

Name:	dnaE2-2
Ordered Locus Names:	Atu5100
ORF Names:	AGR_pAT_bx5

Encoded on

Plasmid AT

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Plasmid Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1085	1085	Error-prone DNA polymerase 2 HAMAP MF_01902		PRO_0000103363

Sequences

	Sequence		Length	Mass (Da)
	Q8UKK2 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 84C00A4E6B85DFB9	FASTA	1,085	121,645
	10 20 30 40 50 60 MSYAELQVTT HFSFLRGASS AQELFETAKA LGIQAIGVVD RNSLAGIVRA LEASRATDIR 70 80 90 100 110 120 LVVGCRLDLT DGMSLLVYPT DRAAYSRLTR LITLGKSRGG KNNCILHWDD VIAYSRGMIG 130 140 150 160 170 180 VLVPDLPDAT CAAQLRRIAE AFGDRAYVSL CLRRRQNDQM RLHEISNLAA RFKVKTVVTN 190 200 210 220 230 240 DVLFHEPGRR QLQDIVTCIR HNTTIDDVGF ERERHADRYL KPPEEMERLF SRYSEALART 250 260 270 280 290 300 LEIVRRCKFS LEELTYQYPE EAIVPGKDAQ ASLEHYVWEC APDRYPEGLP QDVLKTVRHE 310 320 330 340 350 360 LDLIRTMKYA PYFLTVFSIV RFARSQGILC QGRGSAANSA VCYILGITSI DPSTNDLLFE 370 380 390 400 410 420 RFVSQERDEP PDIDVDFEHE RREEVIQWIY KTYTKDKAAL CATVTRYRAK GAIRDVGKAL 430 440 450 460 470 480 GLPEDVIKAL SSGMWSWSEE VPDRNIRELN LNPDDRRLAL TLKLAQQLMG APRHLGQHPG 490 500 510 520 530 540 GFVLTHDRLD DLVPIEPATM KDRQIIEWDK DDVEALKFMK VDVLALGMLT CMAKAFDLIR 550 560 570 580 590 600 EHKGQQLDLS NIEQEDAATY AMIRKADTLG TFQIESRAQM AMLPRLKPRT FYDLVVQVAI 610 620 630 640 650 660 VRPGPIQGDM VHPYLRRREG KEPVEYPTPE LEAVLGKTLG VPLFQESAMR VAMVCAGFTG 670 680 690 700 710 720 GEADQLRKSM ATFKFTGGVS RFKDKLVSGM VKNGYSPEFA EKTFSQLEGF GSYGFPESHA 730 740 750 760 770 780 ASFALIAYAS NYIKCHYPDV FCAALLNSQP MGFYAPAQIV GDAIKHGVEV RPVCVNRSRW 790 800 810 820 830 840 DCTLERIEGS DRHAVRLGFR QVKGLAVADA ARIVAARMNN PFASVDDMWR RSSVPTEALV 850 860 870 880 890 900 QLAEADAFLP SLKLERRDAL WAIKALRDEP LPLFAAAAER EATAIAEQQE PEVALRQMTD 910 920 930 940 950 960 GHNVIEDYSH TGLTLRQHPV AFLRRDLSAR NIIPCAEAMN ARDGRWVYTA GLVLVRQKPG 970 980 990 1000 1010 1020 SAKGVMFITI EDETGPANIV VWPSLFEKRR SVVLGSSMMA INGRIQREGE VVHLVAQQLF 1030 1040 1050 1060 1070 1080 DLSADLVGLA DRDTGFRLPA GRGDEFAHGG GGPDSRDRQK PVVPRDMFTP DLHIDTLKIK SRNFQ

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007872 Genomic DNA. Translation: AAK90476.1.
PIR	AH3171.
RefSeq	NP_396035.1. NC_003064.2.
3D structure databases
ProteinModelPortal	Q8UKK2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UKK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1136873.
GenomeReviews	Gene locus Atu5100 in contig AE007872_GR.
KEGG	atu:Atu5100.
PATRIC	20819397. VBIAgrTum91616_4793.
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	YRAKGAI.
ProtClustDB	CLSK881842.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU5100-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNE22_AGRT5

Accession

Primary (citable) accession number: Q8UKK2
Secondary accession number(s): Q7D3X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program