Reviewed, UniProtKB/Swiss-Prot Q8UJC5 (AROE_AGRT5)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	Atu0003
ORF Names:	AGR_C_3

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Shikimate dehydrogenase HAMAP MF_00222		PRO_0000135988
Regions
	Nucleotide binding	137 – 141	5	NADP By similarity
Sites
	Active site	76	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8UJC5-1 [UniParc]. Last modified October 19, 2002. Version 2. Checksum: A099CE911F493D3C	FASTA	286	31,237
	10 20 30 40 50 60 MADSRETLTI NAFVVGYPIK HSRSPIIHSY WLKKFGIAGS YTAVEVSPDD FPKFIATLKE 70 80 90 100 110 120 GKPGAAVGGN ATIPHKEAAY RLADHPDALA EELGAANTIW MEEGKLHATN TDGYGFVSNL 130 140 150 160 170 180 DERHPGWDKT QRAVVFGAGG ASRAVIQSLR DRDVAEIHVV NRTVERAREL ADRFGPRVFS 190 200 210 220 230 240 HPQAALQEVM HGAGLFVNTT SLGMNGTEAP HLDFSHLAAN AVVTDIVYVP LKTPILNMAQ 250 260 270 280 EQGIATVDGL GMLLHQAKPG FRRWFGRIPE VDETLRSLII ADMEKH

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK85828.2.
PIR	AD2577. C97359.
RefSeq	NP_353043.2.
3D structure databases
SMR	Q8UJC5. Positions 12-273.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UJC5.
Genome annotation databases
GeneID	1132041.
GenomeReviews	Gene locus Atu0003 in contig AE007869_GR.
KEGG	atc:AGR_C_3. atu:Atu0003.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	ANTIWME.
PhylomeDB	Q8UJC5.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU0003-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_AGRT5

Accession

Primary (citable) accession number: Q8UJC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	February 9, 2010
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)