Adenosylhomocysteinase - Agrobacterium tumefaciens (strain C58 / ATCC 33970)

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Q8UJ99 (SAHH_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 70. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Adenosylhomocysteinase
EC=3.3.1.1

Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	Atu0029
ORF Names:	AGR_C_46

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine By similarity. HAMAP MF_00563
Catalytic activity	S-adenosyl-L-homocysteine + H₂O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 466

466

Adenosylhomocysteinase HAMAP MF_00563

PRO_0000116939

Regions

Nucleotide binding

193 – 195

NAD By similarity

Nucleotide binding

256 – 261

NAD By similarity

Nucleotide binding

335 – 337

NAD By similarity

Sites

Binding site

Substrate By similarity

Binding site

132

Substrate By similarity

Binding site

192

Substrate By similarity

Binding site

222

Substrate By similarity

Binding site

226

Substrate By similarity

Binding site

227

NAD By similarity

Binding site

279

NAD By similarity

Binding site

314

NAD By similarity

Binding site

380

NAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8UJ99 [UniParc].

Last modified June 6, 2002. Version 1.
Checksum: 9A124B3304419F26
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FASTA

466

51,160

        10         20         30         40         50         60 
MSLEKDYIVA DINLAAFGRK ELDIAETEMP GLMSCRKEFG ESKPLKGARI TGSLHMTIQT 

        70         80         90        100        110        120 
GVLIETLKEL GAEIRWASCN IFSTQDHAAA AIAAAGIPVF AVKGESLTEY WEYTDKIFQW 

       130        140        150        160        170        180 
TDGGLSNMIL DDGGDATMYI LLGARAEAGE DVLSNPGSEE EEILFAQINK RLKASPGWFT 

       190        200        210        220        230        240 
KQRDALKGVT EETTTGVHRL YDLAKKGLLP FPAINVNDSV TKSKFDNKYG CKESLVDGIR 

       250        260        270        280        290        300 
RATDVMMAGK VAVVCGYGDV GKGSAASLQG AGARVKVTEI DPICALQAAM DGFEVVRLED 

       310        320        330        340        350        360 
VVSSADIFIT TTGNKDVIRI EHMREMKDMA IVGNIGHFDN EIQVASLRNL KWTNIKPQVD 

       370        380        390        400        410        420 
MIEFPKGNRI ILLSEGRLLN LGNATGHPSF VMSASFTNQV LGQIELFTKP GEYKNEVYVL 

       430        440        450        460 
PKHLDEKVAR LHLEKLGVRL TELSDLQADY IGISKQGPFK AEHYRY

« Hide

References

[1]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
[2]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK85853.1.
PIR	AF2580. D97362.
RefSeq	NP_353068.1. NC_003062.2.
3D structure databases
ProteinModelPortal	Q8UJ99.
SMR	Q8UJ99. Positions 5-466.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UJ99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1132067.
GenomeReviews	Gene locus Atu0029 in contig AE007869_GR.
KEGG	atu:Atu0029.
PATRIC	20809746. VBIAgrTum91616_0029.
Phylogenomic databases
eggNOG	COG0499.
HOGENOM	HBG352029.
OMA	SAQVWVT.
PhylomeDB	Q8UJ99.
ProtClustDB	PRK05476.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU0029-MONOMER.
Family and domain databases
HAMAP	MF_00563. AdoHcyase. [Tree]
InterPro	IPR000043. Adenosylhomocysteinase. IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
KO	K01251.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMART	SM00996. AdoHcyase. 1 hit. SM00997. AdoHcyase_NAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00936. AhcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_AGRT5

Accession

Primary (citable) accession number: Q8UJ99

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 6, 2002
Last modified:	January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents