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Q8UJ85

- MASZ_AGRT5

UniProt

Q8UJ85 - MASZ_AGRT5

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Protein

Malate synthase G

Gene

glcB

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei282 – 2821Acetyl-CoAUniRule annotation
Binding sitei319 – 3191Acetyl-CoAUniRule annotation
Active sitei346 – 3461Proton acceptorUniRule annotation
Binding sitei346 – 3461GlyoxylateUniRule annotation
Metal bindingi438 – 4381MagnesiumUniRule annotation
Binding sitei438 – 4381GlyoxylateUniRule annotation
Metal bindingi466 – 4661MagnesiumUniRule annotation
Binding sitei547 – 5471Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei637 – 6371Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Atu0047
ORF Names:AGR_C_78
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 731731Malate synthase GPRO_0000166879Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei623 – 6231Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu0047.

Structurei

3D structure databases

ProteinModelPortaliQ8UJ85.
SMRiQ8UJ85. Positions 18-730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1332Acetyl-CoA bindingUniRule annotation
Regioni463 – 4664Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UJ85-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSEYKEAHV SRTDKFGLSI DDRLYAFLTD EVLPGTGLDS ETFFEGFSAI
60 70 80 90 100
VHELSPKNRE LLAKRDALQE KIDGWYRENG APSDFDAYEA FLKEIGYLLP
110 120 130 140 150
EGPGFKVETN NVDPEIAVVA GPQLVVPVMN ARYALNAANA RWGSLYDALY
160 170 180 190 200
GTDAISDADG AEKGRGYNPK RGDKVIAWAR NFLDESAPLE TGSWSDVTGF
210 220 230 240 250
NIADGLLQLA IGAATTGLKD AVQFKGFSGE AAKPATILLG KNGLHTEIVI
260 270 280 290 300
DPSTEIGKSD RAGISDVILE SALTTIMDCE DSVAAVDAED KVLVYGNWLG
310 320 330 340 350
LMRGDLTEAV SKGGNTFTRR LNPDRYYTAP DGSALTLPGR SLMLVRNVGH
360 370 380 390 400
LMTNPAILDR DGRDVPEGIM DAVVTALIAL YDVGPSGRRQ NSRAGSMYVV
410 420 430 440 450
KPKMHGPEEV AFANEIFARV ENLVGMAPNT MKMGIMDEER RTTVNLKESI
460 470 480 490 500
RAAKDRVVFI NTGFLDRTGD EIHTSMEAGP MIRKGDMKQA AWIAAYENWN
510 520 530 540 550
VDIGLECGLS GHAQIGKGMW AMPDLMAAML EQKIAHPKAG ANTAWVPSPT
560 570 580 590 600
AATLHATHYH KVDVAAVQEG LKSRGRAKLS DILSVPVAPR PNWTPEEIQR
610 620 630 640 650
ELDNNAQGIL GYVVRWVDQG VGCSKVPDIN NIGLMEDRAT LRISAQHMAN
660 670 680 690 700
WLRHGVVTEA QIIKTMKRMA AVVDTQNAGD PAYLPMASDF DGSVAFQAAV
710 720 730
ELVLKGREQP NGYTEPVLHR RRLELKAKQA G
Length:731
Mass (Da):79,520
Last modified:April 30, 2003 - v2
Checksum:i69F304D5D6F8EFFB
GO

Sequence cautioni

The sequence AAK85871.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK85871.1. Different initiation.
PIRiAH2582.
F97364.
RefSeqiNP_353086.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK85871; AAK85871; Atu0047.
GeneIDi1132085.
KEGGiatu:Atu0047.
PATRICi20809782. VBIAgrTum91616_0047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK85871.1 . Different initiation.
PIRi AH2582.
F97364.
RefSeqi NP_353086.1. NC_003062.2.

3D structure databases

ProteinModelPortali Q8UJ85.
SMRi Q8UJ85. Positions 18-730.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176299.Atu0047.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK85871 ; AAK85871 ; Atu0047 .
GeneIDi 1132085.
KEGGi atu:Atu0047.
PATRICi 20809782. VBIAgrTum91616_0047.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiMASZ_AGRT5
AccessioniPrimary (citable) accession number: Q8UJ85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: October 29, 2014
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3