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Q8UJ85

- MASZ_AGRT5

UniProt

Q8UJ85 - MASZ_AGRT5

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Protein
Malate synthase G
Gene
glcB, Atu0047, AGR_C_78
Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei125 – 1251Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei282 – 2821Acetyl-CoA By similarity
Binding sitei319 – 3191Acetyl-CoA By similarity
Active sitei346 – 3461Proton acceptor By similarity
Binding sitei346 – 3461Glyoxylate By similarity
Metal bindingi438 – 4381Magnesium By similarity
Binding sitei438 – 4381Glyoxylate By similarity
Metal bindingi466 – 4661Magnesium By similarity
Binding sitei547 – 5471Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei637 – 6371Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Ordered Locus Names:Atu0047
ORF Names:AGR_C_78
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 731731Malate synthase GUniRule annotation
PRO_0000166879Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei623 – 6231Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu0047.

Structurei

3D structure databases

ProteinModelPortaliQ8UJ85.
SMRiQ8UJ85. Positions 18-730.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni132 – 1332Acetyl-CoA binding By similarity
Regioni463 – 4664Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UJ85-1 [UniParc]FASTAAdd to Basket

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MPSEYKEAHV SRTDKFGLSI DDRLYAFLTD EVLPGTGLDS ETFFEGFSAI    50
VHELSPKNRE LLAKRDALQE KIDGWYRENG APSDFDAYEA FLKEIGYLLP 100
EGPGFKVETN NVDPEIAVVA GPQLVVPVMN ARYALNAANA RWGSLYDALY 150
GTDAISDADG AEKGRGYNPK RGDKVIAWAR NFLDESAPLE TGSWSDVTGF 200
NIADGLLQLA IGAATTGLKD AVQFKGFSGE AAKPATILLG KNGLHTEIVI 250
DPSTEIGKSD RAGISDVILE SALTTIMDCE DSVAAVDAED KVLVYGNWLG 300
LMRGDLTEAV SKGGNTFTRR LNPDRYYTAP DGSALTLPGR SLMLVRNVGH 350
LMTNPAILDR DGRDVPEGIM DAVVTALIAL YDVGPSGRRQ NSRAGSMYVV 400
KPKMHGPEEV AFANEIFARV ENLVGMAPNT MKMGIMDEER RTTVNLKESI 450
RAAKDRVVFI NTGFLDRTGD EIHTSMEAGP MIRKGDMKQA AWIAAYENWN 500
VDIGLECGLS GHAQIGKGMW AMPDLMAAML EQKIAHPKAG ANTAWVPSPT 550
AATLHATHYH KVDVAAVQEG LKSRGRAKLS DILSVPVAPR PNWTPEEIQR 600
ELDNNAQGIL GYVVRWVDQG VGCSKVPDIN NIGLMEDRAT LRISAQHMAN 650
WLRHGVVTEA QIIKTMKRMA AVVDTQNAGD PAYLPMASDF DGSVAFQAAV 700
ELVLKGREQP NGYTEPVLHR RRLELKAKQA G 731
Length:731
Mass (Da):79,520
Last modified:April 30, 2003 - v2
Checksum:i69F304D5D6F8EFFB
GO

Sequence cautioni

The sequence AAK85871.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK85871.1. Different initiation.
PIRiAH2582.
F97364.
RefSeqiNP_353086.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK85871; AAK85871; Atu0047.
GeneIDi1132085.
KEGGiatu:Atu0047.
PATRICi20809782. VBIAgrTum91616_0047.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK85871.1 . Different initiation.
PIRi AH2582.
F97364.
RefSeqi NP_353086.1. NC_003062.2.

3D structure databases

ProteinModelPortali Q8UJ85.
SMRi Q8UJ85. Positions 18-730.
ModBasei Search...

Protein-protein interaction databases

STRINGi 176299.Atu0047.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK85871 ; AAK85871 ; Atu0047 .
GeneIDi 1132085.
KEGGi atu:Atu0047.
PATRICi 20809782. VBIAgrTum91616_0047.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiMASZ_AGRT5
AccessioniPrimary (citable) accession number: Q8UJ85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 14, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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