Reviewed, UniProtKB/Swiss-Prot Q8UJ85 (MASZ_AGRT5)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Atu0047
ORF Names:	AGR_C_78

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	Malate synthase G HAMAP MF_00641		PRO_0000166879
Sites
	Active site	346	1	Proton acceptor By similarity
	Active site	637	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UJ85-1 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 69F304D5D6F8EFFB	FASTA	731	79,520
	10 20 30 40 50 60 MPSEYKEAHV SRTDKFGLSI DDRLYAFLTD EVLPGTGLDS ETFFEGFSAI VHELSPKNRE 70 80 90 100 110 120 LLAKRDALQE KIDGWYRENG APSDFDAYEA FLKEIGYLLP EGPGFKVETN NVDPEIAVVA 130 140 150 160 170 180 GPQLVVPVMN ARYALNAANA RWGSLYDALY GTDAISDADG AEKGRGYNPK RGDKVIAWAR 190 200 210 220 230 240 NFLDESAPLE TGSWSDVTGF NIADGLLQLA IGAATTGLKD AVQFKGFSGE AAKPATILLG 250 260 270 280 290 300 KNGLHTEIVI DPSTEIGKSD RAGISDVILE SALTTIMDCE DSVAAVDAED KVLVYGNWLG 310 320 330 340 350 360 LMRGDLTEAV SKGGNTFTRR LNPDRYYTAP DGSALTLPGR SLMLVRNVGH LMTNPAILDR 370 380 390 400 410 420 DGRDVPEGIM DAVVTALIAL YDVGPSGRRQ NSRAGSMYVV KPKMHGPEEV AFANEIFARV 430 440 450 460 470 480 ENLVGMAPNT MKMGIMDEER RTTVNLKESI RAAKDRVVFI NTGFLDRTGD EIHTSMEAGP 490 500 510 520 530 540 MIRKGDMKQA AWIAAYENWN VDIGLECGLS GHAQIGKGMW AMPDLMAAML EQKIAHPKAG 550 560 570 580 590 600 ANTAWVPSPT AATLHATHYH KVDVAAVQEG LKSRGRAKLS DILSVPVAPR PNWTPEEIQR 610 620 630 640 650 660 ELDNNAQGIL GYVVRWVDQG VGCSKVPDIN NIGLMEDRAT LRISAQHMAN WLRHGVVTEA 670 680 690 700 710 720 QIIKTMKRMA AVVDTQNAGD PAYLPMASDF DGSVAFQAAV ELVLKGREQP NGYTEPVLHR 730 RRLELKAKQA G

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK85871.1. Different initiation.
PIR	AH2582. F97364.
RefSeq	NP_353086.1.
3D structure databases
HSSP	HSSP built from PDB template 1N8I based on UniProtKB Q50596.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UJ85.
Genome annotation databases
GeneID	1132085.
GenomeReviews	Gene locus Atu0047 in contig AE007869_GR.
KEGG	atc:AGR_C_78. atu:Atu0047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UJ85.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_AGRT5

Accession

Primary (citable) accession number: Q8UJ85

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	September 22, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)