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Q8UJ85

- MASZ_AGRT5

UniProt

Q8UJ85 - MASZ_AGRT5

Protein

Malate synthase G

Gene

glcB

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (30 Apr 2003)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei125 – 1251Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei282 – 2821Acetyl-CoAUniRule annotation
    Binding sitei319 – 3191Acetyl-CoAUniRule annotation
    Active sitei346 – 3461Proton acceptorUniRule annotation
    Binding sitei346 – 3461GlyoxylateUniRule annotation
    Metal bindingi438 – 4381MagnesiumUniRule annotation
    Binding sitei438 – 4381GlyoxylateUniRule annotation
    Metal bindingi466 – 4661MagnesiumUniRule annotation
    Binding sitei547 – 5471Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei637 – 6371Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Atu0047
    ORF Names:AGR_C_78
    OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
    Taxonomic identifieri176299 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    ProteomesiUP000000813: Chromosome circular

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 731731Malate synthase GPRO_0000166879Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei623 – 6231Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi176299.Atu0047.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8UJ85.
    SMRiQ8UJ85. Positions 18-730.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni132 – 1332Acetyl-CoA bindingUniRule annotation
    Regioni463 – 4664Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8UJ85-1 [UniParc]FASTAAdd to Basket

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    MPSEYKEAHV SRTDKFGLSI DDRLYAFLTD EVLPGTGLDS ETFFEGFSAI    50
    VHELSPKNRE LLAKRDALQE KIDGWYRENG APSDFDAYEA FLKEIGYLLP 100
    EGPGFKVETN NVDPEIAVVA GPQLVVPVMN ARYALNAANA RWGSLYDALY 150
    GTDAISDADG AEKGRGYNPK RGDKVIAWAR NFLDESAPLE TGSWSDVTGF 200
    NIADGLLQLA IGAATTGLKD AVQFKGFSGE AAKPATILLG KNGLHTEIVI 250
    DPSTEIGKSD RAGISDVILE SALTTIMDCE DSVAAVDAED KVLVYGNWLG 300
    LMRGDLTEAV SKGGNTFTRR LNPDRYYTAP DGSALTLPGR SLMLVRNVGH 350
    LMTNPAILDR DGRDVPEGIM DAVVTALIAL YDVGPSGRRQ NSRAGSMYVV 400
    KPKMHGPEEV AFANEIFARV ENLVGMAPNT MKMGIMDEER RTTVNLKESI 450
    RAAKDRVVFI NTGFLDRTGD EIHTSMEAGP MIRKGDMKQA AWIAAYENWN 500
    VDIGLECGLS GHAQIGKGMW AMPDLMAAML EQKIAHPKAG ANTAWVPSPT 550
    AATLHATHYH KVDVAAVQEG LKSRGRAKLS DILSVPVAPR PNWTPEEIQR 600
    ELDNNAQGIL GYVVRWVDQG VGCSKVPDIN NIGLMEDRAT LRISAQHMAN 650
    WLRHGVVTEA QIIKTMKRMA AVVDTQNAGD PAYLPMASDF DGSVAFQAAV 700
    ELVLKGREQP NGYTEPVLHR RRLELKAKQA G 731
    Length:731
    Mass (Da):79,520
    Last modified:April 30, 2003 - v2
    Checksum:i69F304D5D6F8EFFB
    GO

    Sequence cautioni

    The sequence AAK85871.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007869 Genomic DNA. Translation: AAK85871.1. Different initiation.
    PIRiAH2582.
    F97364.
    RefSeqiNP_353086.1. NC_003062.2.

    Genome annotation databases

    EnsemblBacteriaiAAK85871; AAK85871; Atu0047.
    GeneIDi1132085.
    KEGGiatu:Atu0047.
    PATRICi20809782. VBIAgrTum91616_0047.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007869 Genomic DNA. Translation: AAK85871.1 . Different initiation.
    PIRi AH2582.
    F97364.
    RefSeqi NP_353086.1. NC_003062.2.

    3D structure databases

    ProteinModelPortali Q8UJ85.
    SMRi Q8UJ85. Positions 18-730.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176299.Atu0047.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK85871 ; AAK85871 ; Atu0047 .
    GeneIDi 1132085.
    KEGGi atu:Atu0047.
    PATRICi 20809782. VBIAgrTum91616_0047.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.

    Entry informationi

    Entry nameiMASZ_AGRT5
    AccessioniPrimary (citable) accession number: Q8UJ85
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3