Q8UJ05 (ADE_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenine deaminase
Short name=ADE
EC=3.5.4.2

Alternative name(s):
Adenine aminohydrolase
Short name=AAH

Gene names

Ordered Locus Names:	Atu0136
ORF Names:	AGR_C_218

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism By similarity. HAMAP MF_01962
Catalytic activity	Adenine + H2O = hypoxanthine + NH3. HAMAP MF_01962
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01962
Sequence similarities	Belongs to the adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	adenine deaminase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Adenine deaminase HAMAP MF_01962		PRO_0000194361
Sites
	Active site	192	1	Proton donor By similarity
	Metal binding	11	1	Zinc; catalytic By similarity
	Metal binding	13	1	Zinc; catalytic By similarity
	Metal binding	189	1	Zinc; catalytic By similarity
	Metal binding	270	1	Zinc; catalytic By similarity
	Binding site	271	1	Substrate By similarity
	Site	213	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UJ05 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 6CF30B967C47513D	FASTA	325	35,127
	10 20 30 40 50 60 MTSHLLKAEI HCHLEGAAPP ALVVKQAEKY GIDTSGFLRD GQYVWSDFAE FIQCYDAVAQ 70 80 90 100 110 120 VFKSDEDYAV LTETYLTELA EANTIYSELI ISPDHGDRIG LGADAYLAGV AEGIRIAKEK 130 140 150 160 170 180 TGIETRIIVT GERHFGPERV IAAAEYAARI RHPLVTGFNM AGEERMGRVA DYARAFDIAR 190 200 210 220 230 240 DAGLGLTIHA GEVCGPESVA DALDLVKPSR IGHGVRAIED AALISRLVET GTVLEVCPGS 250 260 270 280 290 300 NIALSVYPDF ASHPLKALSD AGVRVCISSD DPPFFFTSLA REYALAADAF GFNDAEINRM 310 320 TRTALECAFV DEATRERLLA RLGDA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK85957.1.
PIR	AB2593. D97375.
RefSeq	NP_353172.1. NC_003062.2.
3D structure databases
ProteinModelPortal	Q8UJ05.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UJ05.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1132174.
GenomeReviews	Gene locus Atu0136 in contig AE007869_GR.
KEGG	atu:Atu0136.
PATRIC	20809952. VBIAgrTum91616_0127.
Phylogenomic databases
eggNOG	COG1816.
HOGENOM	HBG630382.
OMA	IVLECCP.
PhylomeDB	Q8UJ05.
ProtClustDB	PRK09358.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU0136-MONOMER.
Family and domain databases
HAMAP	MF_01962. Adenine_deaminase. [Tree]
InterPro	IPR001365. A/AMP_deaminase_dom. IPR006330. A_deaminase. [Graphical view]
KO	K01488.
PANTHER	PTHR11409:SF21. PTHR11409:SF21. 1 hit.
Pfam	PF00962. A_deaminase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01430. Aden_deam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ADE_AGRT5

Accession

Primary (citable) accession number: Q8UJ05

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program