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Protein

Nicotinate phosphoribosyltransferase

Gene

pncB

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.UniRule annotation

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Nicotinate phosphoribosyltransferase (pncB)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes nicotinate D-ribonucleotide from nicotinate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00253; UER00457.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate phosphoribosyltransferaseUniRule annotation (EC:6.3.4.21UniRule annotation)
Short name:
NAPRTaseUniRule annotation
Gene namesi
Name:pncBUniRule annotation
Ordered Locus Names:Atu0213
ORF Names:AGR_C_367
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002058171 – 434Nicotinate phosphoribosyltransferaseAdd BLAST434

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu0213.

Structurei

Secondary structure

1434
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 7Combined sources6
Helixi29 – 41Combined sources13
Beta strandi46 – 54Combined sources9
Turni61 – 63Combined sources3
Helixi66 – 76Combined sources11
Helixi83 – 91Combined sources9
Beta strandi94 – 96Combined sources3
Helixi103 – 110Combined sources8
Beta strandi118 – 121Combined sources4
Beta strandi126 – 130Combined sources5
Helixi134 – 137Combined sources4
Helixi138 – 140Combined sources3
Helixi141 – 157Combined sources17
Helixi162 – 183Combined sources22
Beta strandi191 – 193Combined sources3
Turni196 – 198Combined sources3
Helixi202 – 216Combined sources15
Helixi217 – 219Combined sources3
Beta strandi220 – 225Combined sources6
Helixi226 – 232Combined sources7
Helixi243 – 251Combined sources9
Helixi255 – 272Combined sources18
Helixi275 – 277Combined sources3
Helixi287 – 292Combined sources6
Helixi296 – 300Combined sources5
Beta strandi301 – 304Combined sources4
Helixi311 – 324Combined sources14
Helixi329 – 331Combined sources3
Beta strandi332 – 336Combined sources5
Helixi342 – 352Combined sources11
Turni353 – 355Combined sources3
Beta strandi356 – 362Combined sources7
Helixi364 – 367Combined sources4
Beta strandi386 – 393Combined sources8
Helixi405 – 407Combined sources3
Helixi412 – 422Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YBEX-ray2.50A/B1-434[»]
ProteinModelPortaliQ8UIS9.
SMRiQ8UIS9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8UIS9.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107RH7. Bacteria.
COG1488. LUCA.
HOGENOMiHOG000284929.
KOiK00763.
OMAiKLTMQCA.

Family and domain databases

HAMAPiMF_00570. NAPRTase. 1 hit.
InterProiIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR01514. NAPRTase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UIS9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTDIATRV HNHTWKLDPI VRSLIDTDFY KLLMLQMIWK LYPEVDATFS
60 70 80 90 100
LINRTKTVRL AEEIDEMELR EQLDHARTLR LSKKENIWLA GNTFYGRSQI
110 120 130 140 150
FEPEFLSWLS SYQLPEYELF KRDGQYELNF HGRWMDTTLW EIPALSIINE
160 170 180 190 200
LRSRSAMRSL GYFTLDVLYA RAKAKMWEKV ERLRELPGLR ISDFGTRRRH
210 220 230 240 250
SFLWQRWCVE ALKEGIGPAF TGTSNVLLAM DSDLEAVGTN AHELPMVVAA
260 270 280 290 300
LAQTNEELAA APYQVLKDWN RLYGGNLLIV LPDAFGTAAF LRNAPEWVAD
310 320 330 340 350
WTGFRPDSAP PIEGGEKIIE WWRKMGRDPR TKMLIFSDGL DVDAIVDTYR
360 370 380 390 400
HFEGRVRMSF GWGTNLTNDF AGCAPKTIAS LKPISIVCKV SDANGRPAVK
410 420 430
LSDNPQKATG DPAEVERYLK FFGEEDHKEQ KVLV
Length:434
Mass (Da):49,902
Last modified:July 11, 2002 - v2
Checksum:iABC37DD605AE647F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86031.2.
PIRiAF2602.
F97384.
RefSeqiNP_353246.2. NC_003062.2.
WP_010970734.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK86031; AAK86031; Atu0213.
GeneIDi1132251.
KEGGiatu:Atu0213.
PATRICi20810107. VBIAgrTum91616_0204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86031.2.
PIRiAF2602.
F97384.
RefSeqiNP_353246.2. NC_003062.2.
WP_010970734.1. NC_003062.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YBEX-ray2.50A/B1-434[»]
ProteinModelPortaliQ8UIS9.
SMRiQ8UIS9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu0213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK86031; AAK86031; Atu0213.
GeneIDi1132251.
KEGGiatu:Atu0213.
PATRICi20810107. VBIAgrTum91616_0204.

Phylogenomic databases

eggNOGiENOG4107RH7. Bacteria.
COG1488. LUCA.
HOGENOMiHOG000284929.
KOiK00763.
OMAiKLTMQCA.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00457.

Miscellaneous databases

EvolutionaryTraceiQ8UIS9.

Family and domain databases

HAMAPiMF_00570. NAPRTase. 1 hit.
InterProiIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR01514. NAPRTase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPNCB_AGRFC
AccessioniPrimary (citable) accession number: Q8UIS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 11, 2002
Last modified: November 2, 2016
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.