Reviewed, UniProtKB/Swiss-Prot Q8UIS9 (PNCB_AGRT5)

Names and origin

Protein names

Recommended name:
    Nicotinate phosphoribosyltransferase
      Short name=NAPRTase
    EC=2.4.2.11

Gene names

Name:	pncB
Ordered Locus Names:	Atu0213
ORF Names:	AGR_C_367

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00570
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. HAMAP MF_00570
Subcellular location	Cytoplasm By similarity HAMAP MF_00570.
Sequence similarities	Belongs to the NAPRTase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP nicotinate nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nicotinate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 434

434

Nicotinate phosphoribosyltransferase HAMAP MF_00570

PRO_0000205817

Secondary structure

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434

Helix Strand Turn

Details...

Helix

2 – 7

6

Helix

29 – 41

13

Beta strand

46 – 54

9

Turn

61 – 63

3

Helix

66 – 76

11

Helix

83 – 91

9

Beta strand

94 – 96

3

Helix

103 – 110

8

Beta strand

118 – 121

4

Beta strand

126 – 130

5

Helix

134 – 137

4

Helix

138 – 140

3

Helix

141 – 157

17

Helix

162 – 183

22

Beta strand

191 – 193

3

Turn

196 – 198

3

Helix

202 – 216

15

Helix

217 – 219

3

Beta strand

220 – 225

6

Helix

226 – 232

7

Helix

243 – 251

9

Helix

255 – 272

18

Helix

275 – 277

3

Helix

287 – 292

6

Helix

296 – 300

5

Beta strand

301 – 304

4

Helix

311 – 324

14

Helix

329 – 331

3

Beta strand

332 – 336

5

Helix

342 – 352

11

Turn

353 – 355

3

Beta strand

356 – 362

7

Helix

364 – 367

4

Beta strand

386 – 393

8

Helix

405 – 407

3

Helix

412 – 422

11

Sequences

	Sequence		Length	Mass (Da)
	Q8UIS9-1 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: ABC37DD605AE647F	FASTA	434	49,902
	10 20 30 40 50 60 MTKTDIATRV HNHTWKLDPI VRSLIDTDFY KLLMLQMIWK LYPEVDATFS LINRTKTVRL 70 80 90 100 110 120 AEEIDEMELR EQLDHARTLR LSKKENIWLA GNTFYGRSQI FEPEFLSWLS SYQLPEYELF 130 140 150 160 170 180 KRDGQYELNF HGRWMDTTLW EIPALSIINE LRSRSAMRSL GYFTLDVLYA RAKAKMWEKV 190 200 210 220 230 240 ERLRELPGLR ISDFGTRRRH SFLWQRWCVE ALKEGIGPAF TGTSNVLLAM DSDLEAVGTN 250 260 270 280 290 300 AHELPMVVAA LAQTNEELAA APYQVLKDWN RLYGGNLLIV LPDAFGTAAF LRNAPEWVAD 310 320 330 340 350 360 WTGFRPDSAP PIEGGEKIIE WWRKMGRDPR TKMLIFSDGL DVDAIVDTYR HFEGRVRMSF 370 380 390 400 410 420 GWGTNLTNDF AGCAPKTIAS LKPISIVCKV SDANGRPAVK LSDNPQKATG DPAEVERYLK 430 FFGEEDHKEQ KVLV

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE007869 Genomic DNA. Translation: AAK86031.2.

PIR

AF2602.
F97384.

RefSeq

NP_353246.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YBE	X-ray	2.50	A/B	1-434	[»]

ModBase

Search...

Protein-protein interaction databases

STRING

Q8UIS9.

Genome annotation databases

GeneID

1132251.

GenomeReviews

Gene locus Atu0213 in contig AE007869_GR.

KEGG

atc:AGR_C_367.
atu:Atu0213.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG1488.

HOGENOM

HBG325503.

OMA

GNTFYGK.

PhylomeDB

Q8UIS9.

Enzyme and pathway databases

BioCyc

ATUM176299-1:ATU0213-MONOMER.

Family and domain databases

HAMAP

MF_00570. NAPRTase.
[Tree]

InterPro

IPR006406. Nic_PRibTrfase.
IPR015977. Nic_PRibTrfase-like.
IPR007229. Nic_PRibTrfase-rel.
[Graphical view]

PANTHER

PTHR11098:SF1. NAPRTase. 1 hit.

Pfam

PF04095. NAPRTase. 1 hit.
[Graphical view]

PIRSF

PIRSF000484. NAPRT. 1 hit.

TIGRFAMs

TIGR01514. NAPRTase. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PNCB_AGRT5

Accession

Primary (citable) accession number: Q8UIS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	July 11, 2002
Last modified:	February 9, 2010
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)