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Protein

Nicotinate phosphoribosyltransferase

Gene

pncB

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of beta-nicotinate D-ribonucleotide from nicotinate and 5-phospho-D-ribose 1-phosphate at the expense of ATP.UniRule annotation

Catalytic activityi

Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. nicotinate-nucleotide diphosphorylase (carboxylating) activity Source: InterPro
  3. nicotinate phosphoribosyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD biosynthetic process Source: UniProtKB-HAMAP
  2. nicotinate nucleotide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase, Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00253; UER00457.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinate phosphoribosyltransferaseUniRule annotation (EC:6.3.4.21UniRule annotation)
Short name:
NAPRTaseUniRule annotation
Gene namesi
Name:pncBUniRule annotation
Ordered Locus Names:Atu0213
ORF Names:AGR_C_367
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Nicotinate phosphoribosyltransferasePRO_0000205817Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu0213.

Structurei

Secondary structure

1
434
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 76Combined sources
Helixi29 – 4113Combined sources
Beta strandi46 – 549Combined sources
Turni61 – 633Combined sources
Helixi66 – 7611Combined sources
Helixi83 – 919Combined sources
Beta strandi94 – 963Combined sources
Helixi103 – 1108Combined sources
Beta strandi118 – 1214Combined sources
Beta strandi126 – 1305Combined sources
Helixi134 – 1374Combined sources
Helixi138 – 1403Combined sources
Helixi141 – 15717Combined sources
Helixi162 – 18322Combined sources
Beta strandi191 – 1933Combined sources
Turni196 – 1983Combined sources
Helixi202 – 21615Combined sources
Helixi217 – 2193Combined sources
Beta strandi220 – 2256Combined sources
Helixi226 – 2327Combined sources
Helixi243 – 2519Combined sources
Helixi255 – 27218Combined sources
Helixi275 – 2773Combined sources
Helixi287 – 2926Combined sources
Helixi296 – 3005Combined sources
Beta strandi301 – 3044Combined sources
Helixi311 – 32414Combined sources
Helixi329 – 3313Combined sources
Beta strandi332 – 3365Combined sources
Helixi342 – 35211Combined sources
Turni353 – 3553Combined sources
Beta strandi356 – 3627Combined sources
Helixi364 – 3674Combined sources
Beta strandi386 – 3938Combined sources
Helixi405 – 4073Combined sources
Helixi412 – 42211Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YBEX-ray2.50A/B1-434[»]
ProteinModelPortaliQ8UIS9.
SMRiQ8UIS9. Positions 1-426.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8UIS9.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAPRTase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1488.
HOGENOMiHOG000284929.
KOiK00763.
OMAiKLTMQCA.
OrthoDBiEOG6X10XB.

Family and domain databases

HAMAPiMF_00570. NAPRTase.
InterProiIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR01514. NAPRTase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UIS9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKTDIATRV HNHTWKLDPI VRSLIDTDFY KLLMLQMIWK LYPEVDATFS
60 70 80 90 100
LINRTKTVRL AEEIDEMELR EQLDHARTLR LSKKENIWLA GNTFYGRSQI
110 120 130 140 150
FEPEFLSWLS SYQLPEYELF KRDGQYELNF HGRWMDTTLW EIPALSIINE
160 170 180 190 200
LRSRSAMRSL GYFTLDVLYA RAKAKMWEKV ERLRELPGLR ISDFGTRRRH
210 220 230 240 250
SFLWQRWCVE ALKEGIGPAF TGTSNVLLAM DSDLEAVGTN AHELPMVVAA
260 270 280 290 300
LAQTNEELAA APYQVLKDWN RLYGGNLLIV LPDAFGTAAF LRNAPEWVAD
310 320 330 340 350
WTGFRPDSAP PIEGGEKIIE WWRKMGRDPR TKMLIFSDGL DVDAIVDTYR
360 370 380 390 400
HFEGRVRMSF GWGTNLTNDF AGCAPKTIAS LKPISIVCKV SDANGRPAVK
410 420 430
LSDNPQKATG DPAEVERYLK FFGEEDHKEQ KVLV
Length:434
Mass (Da):49,902
Last modified:July 11, 2002 - v2
Checksum:iABC37DD605AE647F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86031.2.
PIRiAF2602.
F97384.
RefSeqiNP_353246.2. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK86031; AAK86031; Atu0213.
GeneIDi1132251.
KEGGiatu:Atu0213.
PATRICi20810107. VBIAgrTum91616_0204.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86031.2.
PIRiAF2602.
F97384.
RefSeqiNP_353246.2. NC_003062.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YBEX-ray2.50A/B1-434[»]
ProteinModelPortaliQ8UIS9.
SMRiQ8UIS9. Positions 1-426.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu0213.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK86031; AAK86031; Atu0213.
GeneIDi1132251.
KEGGiatu:Atu0213.
PATRICi20810107. VBIAgrTum91616_0204.

Phylogenomic databases

eggNOGiCOG1488.
HOGENOMiHOG000284929.
KOiK00763.
OMAiKLTMQCA.
OrthoDBiEOG6X10XB.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00457.

Miscellaneous databases

EvolutionaryTraceiQ8UIS9.

Family and domain databases

HAMAPiMF_00570. NAPRTase.
InterProiIPR006406. Nic_PRibTrfase.
IPR007229. Nic_PRibTrfase-Fam.
IPR002638. Quinolinate_PRibosylTrfase_C.
[Graphical view]
PANTHERiPTHR11098. PTHR11098. 1 hit.
PfamiPF04095. NAPRTase. 1 hit.
[Graphical view]
PIRSFiPIRSF000484. NAPRT. 1 hit.
SUPFAMiSSF51690. SSF51690. 1 hit.
TIGRFAMsiTIGR01514. NAPRTase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiPNCB_AGRT5
AccessioniPrimary (citable) accession number: Q8UIS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 11, 2002
Last modified: February 4, 2015
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.