Reviewed, UniProtKB/Swiss-Prot Q8UIL2 (GUAA_AGRT5)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing]
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase
    GMP synthetase

Gene names

Name:	guaA
Ordered Locus Names:	Atu0281
ORF Names:	AGR_C_480

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_0000140086
Regions
	Domain	9 – 202	194	Glutamine amidotransferase type-1
	Domain	234 – 401	168	GMP-binding
	Nucleotide binding	230 – 236	7	ATP By similarity
Sites
	Active site	86	1	Nucleophile By similarity
	Active site	176	1	By similarity
	Active site	178	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UIL2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 581CD3C3B1F64F34	FASTA	525	57,521
	10 20 30 40 50 60 MTQIAHPDSI LIIDFGSQVT QLIARRIREA GVYCEIHPFQ NAAEAFEKLQ PKGVIFSGGP 70 80 90 100 110 120 ASVTAEGSPR APQAVFDSKV PILGICYGQQ TLCTQLGGVV EGGHAAEFGR ADIDIKKASP 130 140 150 160 170 180 LFEGFWEQGK SYPVWMSHGD RVTKLPEGFE VIATSENAPF AIAADEKRHY YTTMFHPEVV 190 200 210 220 230 240 HTPDGGKLLS NFVHKIVGLK SDWTMAAYRA EMIRKIREQV GTGRVLCALS GGVDSSVAAI 250 260 270 280 290 300 LIHEAIGDQL TCVYVDHGLM RLGESEQVVG MFRDHYNIPL VHVDAADLFL GELSGVSDPE 310 320 330 340 350 360 VKRKTIGRLF IEVFEAEAAK IAADGKGAPK FLAQGTLYPD VIESVSFSGG PSVTIKSHHN 370 380 390 400 410 420 VGGLPERMNM QLVEPLRELF KDEVRALGRE LGLPESFIGR HPFPGPGLAI RCPGAITREK 430 440 450 460 470 480 LDILRKADAI YLDEIRKAGL YDTIWQAFAV LLPVQTVGVM GDYRTYDFVC ALRAVTSVDG 490 500 510 520 MTADFYPYDM NFLGRAATRI INEVRGINRV VYDVTSKPPG TIEWE

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK86096.1.
PIR	AI2610. G97392.
RefSeq	NP_353311.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UIL2.
Genome annotation databases
GeneID	1132319.
GenomeReviews	Gene locus Atu0281 in contig AE007869_GR.
KEGG	atc:AGR_C_480. atu:Atu0281.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UIL2.
OMA	DQVMEMF.
Family and domain databases
HAMAP	MF_00344. [Tree]
InterPro	IPR001317. CarbamoylP_synth_GATase. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR014729. Rossmann-like_a/b/a_fold. IPR018318. tRNA_MeTrfase-like. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF03054. tRNA_Me_trans. 1 hit. [Graphical view]
PRINTS	PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit. TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_AGRT5

Accession

Primary (citable) accession number: Q8UIL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)