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Q8UII5 (GSHB_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:Atu0310
ORF Names:AGR_C_538
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197450

Regions

Domain127 – 311185ATP-grasp
Nucleotide binding153 – 20957ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8UII5 [UniParc].

Last modified November 8, 2002. Version 2.
Checksum: 47B4904A760794D5

FASTA31936,052
        10         20         30         40         50         60 
MAKIKNVAIQ MDHVSGINIA GDSTFAISLE AQARGYRLFH YTPERLSMRD GKIYATVEQM 

        70         80         90        100        110        120 
ELRDIKGDHF SLSEPERVDL STMDVIHLRQ DPPFDMAYIT STHLLERIHP KTLVVNDPAW 

       130        140        150        160        170        180 
VRNSPEKIFV TEFADLMPKT LITKDVSEIA RFRNEMGDII LKPLYGNGGA GVFHSARDDR 

       190        200        210        220        230        240 
NFSSLLEMFG QMFREPYIAQ EYLPDVRKGD KRILLVDGEP VGAINRVPAE NDARSNMHAG 

       250        260        270        280        290        300 
GRPEPTELTA REQEICRRIG PALRERGFLF VGIDVIGDYM TEINVTSPTG IREVRKFGGA 

       310 
DVASLLWDAI EKKRDAQDF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007869 Genomic DNA. Translation: AAK86126.2.
PIRAF2614.
E97396.
RefSeqNP_353341.2. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UII5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu0310.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK86126; AAK86126; Atu0310.
GeneID1132348.
KEGGatu:Atu0310.
PATRIC20810305. VBIAgrTum91616_0302.

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAPTCFQEI.
OrthoDBEOG6WMHWB.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycAGRO:ATU0310-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_AGRT5
AccessionPrimary (citable) accession number: Q8UII5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: February 19, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways