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Q8UIF1

- GLND_AGRT5

UniProt

Q8UIF1 - GLND_AGRT5

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciAGRO:ATU0346-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:Atu0346
    ORF Names:AGR_C_606
    OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
    Taxonomic identifieri176299 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    ProteomesiUP000000813: Chromosome circular

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 942942Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192714Add
    BLAST

    Proteomic databases

    PRIDEiQ8UIF1.

    Interactioni

    Protein-protein interaction databases

    STRINGi176299.Atu0346.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8UIF1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini523 – 60684HDUniRule annotationAdd
    BLAST
    Domaini730 – 81182ACT 1UniRule annotationAdd
    BLAST
    Domaini841 – 92080ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 373373UridylyltransferaseAdd
    BLAST
    Regioni374 – 729356Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261779.
    KOiK00990.
    OMAiLYCLWDM.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8UIF1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIKDLDFSD ILDVSALKRD CDVIFKEDSK RIADIKSDLL PIFRRASTEG    50
    REKARELLKA DGSGIDCARR ISWLQDRLIE TLYDLACQHV YPKDAPQIAV 100
    AAVGGYGRGT LAPGSDIDLL FLLPAKNTPD MHKAVEFVLY LLWDLGFKVG 150
    HATRTVDECI RLSKSDMTIR TAILEVRAIC GRKSLTDDLE KRFESEVVTG 200
    TGPEFIAAKL AERDQRHRKA GDTRYLVEPN VKEGKGGLRD LHTLFWIAKY 250
    YYHVRDTADL VKLGVLSRSE LKLFEKADDF LWAVRCQMHF ITGKAEERLS 300
    FDIQREIADA LNYQPRPGLS AVERFMKHYF LVAKDVGDLT RIVCAALEDR 350
    QAKDVPGLSG VLSRFAHRVR KIPGSVEFVE DRGRIALARP DVFKNDPVNL 400
    IRLFHIADIN NLELHPDALR VVTRSLSLIN DELRENEEAN RLFLSILTSR 450
    RDPALTLRRM NEAGVLGKFI PEFGKIVAMM QFNMYHHYTV DEHLIRSVGV 500
    LSEVDKGTAV DAHPLANQLM PGVEEREALY VAVLLHDVAK GRQEDHSIAG 550
    ARVARKLCQR FRLTGKQTET VVWLIEQHLL MSMVAQTRDL HDRKTITDFA 600
    DKVQSMERLK MLLILTVCDI RAVGPGVWNG WKGQLLRSLY YETELLLSGG 650
    FSEVSRKERA QIARQALYDA LEDWGQKARR KYTKLHYEPY LLTVALEDQV 700
    RHTRFMREAD KQEKALSTMV RTHSFHAITE ITVLAPDHPR LLSIITGACA 750
    AAGANIADAQ IFTTSDGRAL DTILINREFP IDEDETRRGA NVGKLIEEVL 800
    SGKQRLPEMI ATRTKSRRKK SAFTIPPSVT ISNGLSNKFT VIEVECLDRP 850
    GLLADMTAVI ADLSLDIHSA RITTFGEKVI DTFYVTDLFG QKVTNDNRQA 900
    SIAQRLKAVM SEQEDELRDR MPNGIIAHPD VAALPAARTA KA 942
    Length:942
    Mass (Da):106,204
    Last modified:June 1, 2002 - v1
    Checksum:iB0EBDF337C7CE945
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007869 Genomic DNA. Translation: AAK86163.2.
    PIRiAB2619.
    B97401.
    RefSeqiNP_353378.2. NC_003062.2.

    Genome annotation databases

    EnsemblBacteriaiAAK86163; AAK86163; Atu0346.
    GeneIDi1132384.
    KEGGiatu:Atu0346.
    PATRICi20810375. VBIAgrTum91616_0337.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007869 Genomic DNA. Translation: AAK86163.2 .
    PIRi AB2619.
    B97401.
    RefSeqi NP_353378.2. NC_003062.2.

    3D structure databases

    ProteinModelPortali Q8UIF1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176299.Atu0346.

    Proteomic databases

    PRIDEi Q8UIF1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK86163 ; AAK86163 ; Atu0346 .
    GeneIDi 1132384.
    KEGGi atu:Atu0346.
    PATRICi 20810375. VBIAgrTum91616_0337.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261779.
    KOi K00990.
    OMAi LYCLWDM.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci AGRO:ATU0346-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.

    Entry informationi

    Entry nameiGLND_AGRT5
    AccessioniPrimary (citable) accession number: Q8UIF1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 2002
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3