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Q8UIF1

- GLND_AGRT5

UniProt

Q8UIF1 - GLND_AGRT5

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, Atu0346, AGR_C_606
Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciAGRO:ATU0346-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Atu0346
ORF Names:AGR_C_606
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 942942Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_0000192714Add
BLAST

Proteomic databases

PRIDEiQ8UIF1.

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu0346.

Structurei

3D structure databases

ProteinModelPortaliQ8UIF1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini523 – 60684HDAdd
BLAST
Domaini730 – 81182ACT 1Add
BLAST
Domaini841 – 92080ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 373373UridylyltransferaseUniRule annotationAdd
BLAST
Regioni374 – 729356Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8UIF1-1 [UniParc]FASTAAdd to Basket

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MAIKDLDFSD ILDVSALKRD CDVIFKEDSK RIADIKSDLL PIFRRASTEG    50
REKARELLKA DGSGIDCARR ISWLQDRLIE TLYDLACQHV YPKDAPQIAV 100
AAVGGYGRGT LAPGSDIDLL FLLPAKNTPD MHKAVEFVLY LLWDLGFKVG 150
HATRTVDECI RLSKSDMTIR TAILEVRAIC GRKSLTDDLE KRFESEVVTG 200
TGPEFIAAKL AERDQRHRKA GDTRYLVEPN VKEGKGGLRD LHTLFWIAKY 250
YYHVRDTADL VKLGVLSRSE LKLFEKADDF LWAVRCQMHF ITGKAEERLS 300
FDIQREIADA LNYQPRPGLS AVERFMKHYF LVAKDVGDLT RIVCAALEDR 350
QAKDVPGLSG VLSRFAHRVR KIPGSVEFVE DRGRIALARP DVFKNDPVNL 400
IRLFHIADIN NLELHPDALR VVTRSLSLIN DELRENEEAN RLFLSILTSR 450
RDPALTLRRM NEAGVLGKFI PEFGKIVAMM QFNMYHHYTV DEHLIRSVGV 500
LSEVDKGTAV DAHPLANQLM PGVEEREALY VAVLLHDVAK GRQEDHSIAG 550
ARVARKLCQR FRLTGKQTET VVWLIEQHLL MSMVAQTRDL HDRKTITDFA 600
DKVQSMERLK MLLILTVCDI RAVGPGVWNG WKGQLLRSLY YETELLLSGG 650
FSEVSRKERA QIARQALYDA LEDWGQKARR KYTKLHYEPY LLTVALEDQV 700
RHTRFMREAD KQEKALSTMV RTHSFHAITE ITVLAPDHPR LLSIITGACA 750
AAGANIADAQ IFTTSDGRAL DTILINREFP IDEDETRRGA NVGKLIEEVL 800
SGKQRLPEMI ATRTKSRRKK SAFTIPPSVT ISNGLSNKFT VIEVECLDRP 850
GLLADMTAVI ADLSLDIHSA RITTFGEKVI DTFYVTDLFG QKVTNDNRQA 900
SIAQRLKAVM SEQEDELRDR MPNGIIAHPD VAALPAARTA KA 942
Length:942
Mass (Da):106,204
Last modified:June 1, 2002 - v1
Checksum:iB0EBDF337C7CE945
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK86163.2.
PIRiAB2619.
B97401.
RefSeqiNP_353378.2. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK86163; AAK86163; Atu0346.
GeneIDi1132384.
KEGGiatu:Atu0346.
PATRICi20810375. VBIAgrTum91616_0337.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK86163.2 .
PIRi AB2619.
B97401.
RefSeqi NP_353378.2. NC_003062.2.

3D structure databases

ProteinModelPortali Q8UIF1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176299.Atu0346.

Proteomic databases

PRIDEi Q8UIF1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK86163 ; AAK86163 ; Atu0346 .
GeneIDi 1132384.
KEGGi atu:Atu0346.
PATRICi 20810375. VBIAgrTum91616_0337.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci AGRO:ATU0346-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiGLND_AGRT5
AccessioniPrimary (citable) accession number: Q8UIF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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