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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei137L-tryptophanUniRule annotation1
Binding sitei208ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 15ATPUniRule annotation3
Nucleotide bindingi21 – 22ATPUniRule annotation2
Nucleotide bindingi149 – 151ATPUniRule annotation3
Nucleotide bindingi217 – 221ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAGRO:ATU0349-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:Atu0349
ORF Names:AGR_C_611
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001365961 – 354Tryptophan--tRNA ligaseAdd BLAST354

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu0349

Structurei

3D structure databases

ProteinModelPortaliQ8UIE8
SMRiQ8UIE8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi14 – 22"HIGH" regionUniRule annotation9
Motifi217 – 221"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
KOiK01867
OMAiQRMTQFK

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8UIE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNAFKPLVFS GVQPTGNLHL GNYLGAIRKF VALQEDNDCI YCVVDMHAIT
60 70 80 90 100
AQLVHSDLKA QTRSIAAAFI AAGIDPVKHI VFNQSAVPQH AELAWVFNCV
110 120 130 140 150
ARIGWMERMT QFKDKSGKNA EQVSLGLLAY PSLMAADILV YRATHVPVGD
160 170 180 190 200
DQKQHLELAR DIAQKFNIDF GGHIRNAGLG VNITVGDEPV HAYFPMVEPL
210 220 230 240 250
IGGPAPRVMS LKDGTKKMSK SDPSDLSRIN LMDDVDAISK KIKKAKTDPD
260 270 280 290 300
ALPSEVEGLK GRPEAENLVG IYAALSDKTK ADVLAEFGGQ QFSTFKPALV
310 320 330 340 350
ELAVNVLAPV NNEMRRLLDD PTHIDAILSQ GGERARTIAE KTMNEVRDII

GFLR
Length:354
Mass (Da):38,647
Last modified:November 8, 2002 - v2
Checksum:i48DDCD4553902834
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA Translation: AAK86166.2
PIRiAE2619
E97401
RefSeqiNP_353381.2, NC_003062.2
WP_010970836.1, NC_003062.2

Genome annotation databases

EnsemblBacteriaiAAK86166; AAK86166; Atu0349
GeneIDi1132387
KEGGiatu:Atu0349
PATRICifig|176299.10.peg.340

Similar proteinsi

Entry informationi

Entry nameiSYW_AGRFC
AccessioniPrimary (citable) accession number: Q8UIE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: March 28, 2018
This is version 89 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health