Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8UIE8 (SYW_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:Atu0349
ORF Names:AGR_C_611
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136596

Regions

Motif14 – 229"HIGH" region HAMAP-Rule MF_00140
Motif217 – 2215"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8UIE8 [UniParc].

Last modified November 8, 2002. Version 2.
Checksum: 48DDCD4553902834

FASTA35438,647
        10         20         30         40         50         60 
MNAFKPLVFS GVQPTGNLHL GNYLGAIRKF VALQEDNDCI YCVVDMHAIT AQLVHSDLKA 

        70         80         90        100        110        120 
QTRSIAAAFI AAGIDPVKHI VFNQSAVPQH AELAWVFNCV ARIGWMERMT QFKDKSGKNA 

       130        140        150        160        170        180 
EQVSLGLLAY PSLMAADILV YRATHVPVGD DQKQHLELAR DIAQKFNIDF GGHIRNAGLG 

       190        200        210        220        230        240 
VNITVGDEPV HAYFPMVEPL IGGPAPRVMS LKDGTKKMSK SDPSDLSRIN LMDDVDAISK 

       250        260        270        280        290        300 
KIKKAKTDPD ALPSEVEGLK GRPEAENLVG IYAALSDKTK ADVLAEFGGQ QFSTFKPALV 

       310        320        330        340        350 
ELAVNVLAPV NNEMRRLLDD PTHIDAILSQ GGERARTIAE KTMNEVRDII GFLR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007869 Genomic DNA. Translation: AAK86166.2.
PIRAE2619.
E97401.
RefSeqNP_353381.2. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UIE8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu0349.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK86166; AAK86166; Atu0349.
GeneID1132387.
KEGGatu:Atu0349.
PATRIC20810381. VBIAgrTum91616_0340.

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059940.
KOK01867.
OMAGWGQFKP.
OrthoDBEOG686NJQ.
ProtClustDBPRK00927.

Enzyme and pathway databases

BioCycAGRO:ATU0349-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_AGRT5
AccessionPrimary (citable) accession number: Q8UIE8
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: February 19, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries