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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei63S-adenosyl-L-methionineUniRule annotation1
Binding sitei88S-adenosyl-L-methionineUniRule annotation1
Binding sitei115S-adenosyl-L-methionineUniRule annotation1
Active sitei137By similarity1
Binding sitei137S-adenosyl-L-methionineUniRule annotation1
Binding sitei141SubstrateUniRule annotation1
Binding sitei173SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAGRO:ATU0363-MONOMER
UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Atu0363
ORF Names:AGR_C_634
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712841 – 232tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST232

Proteomic databases

PRIDEiQ8UID4

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu0363

Structurei

3D structure databases

ProteinModelPortaliQ8UID4
SMRiQ8UID4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni211 – 214Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073969
KOiK03439
OMAiPDPWHKS

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

Q8UID4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEERRSRAT EAFFGRRKGK PLRNQQVDTI ENLLPLLKID LESVPPQNLV
60 70 80 90 100
ALFPADVRSI RLEIGFGGGE HLAHRAVENP ETGFIGVEPF VNSMAKLLAT
110 120 130 140 150
VRERELMNIR LYDDDATQLL DWLPEGSIDH IDLLYPDPWP KKKHWKRRFV
160 170 180 190 200
SDVNLARFHR VLKPGGKFCF ASDIDTYVNW TLQHCARHGG FEWTATSADD
210 220 230
WRTPYANWPG TRYENKAKRE GRSSAYLTFI RR
Length:232
Mass (Da):26,864
Last modified:June 1, 2002 - v1
Checksum:i7F8A29F4E1F00423
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA Translation: AAK86180.1
PIRiAC2621
C97403
RefSeqiNP_353395.1, NC_003062.2
WP_006310208.1, NC_003062.2

Genome annotation databases

EnsemblBacteriaiAAK86180; AAK86180; Atu0363
GeneIDi1132401
KEGGiatu:Atu0363
PATRICifig|176299.10.peg.354

Entry informationi

Entry nameiTRMB_AGRFC
AccessioniPrimary (citable) accession number: Q8UID4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 1, 2002
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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