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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei63 – 631S-adenosyl-L-methionineUniRule annotation
Binding sitei88 – 881S-adenosyl-L-methionineUniRule annotation
Binding sitei115 – 1151S-adenosyl-L-methionineUniRule annotation
Active sitei137 – 1371By similarity
Binding sitei137 – 1371S-adenosyl-L-methionineUniRule annotation
Binding sitei141 – 1411SubstrateUniRule annotation
Binding sitei173 – 1731SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAGRO:ATU0363-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Atu0363
ORF Names:AGR_C_634
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813 Componenti: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 232232tRNA (guanine-N(7)-)-methyltransferasePRO_0000171284Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu0363.

Structurei

3D structure databases

ProteinModelPortaliQ8UID4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni211 – 2144Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiIGCEPFV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8UID4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEERRSRAT EAFFGRRKGK PLRNQQVDTI ENLLPLLKID LESVPPQNLV
60 70 80 90 100
ALFPADVRSI RLEIGFGGGE HLAHRAVENP ETGFIGVEPF VNSMAKLLAT
110 120 130 140 150
VRERELMNIR LYDDDATQLL DWLPEGSIDH IDLLYPDPWP KKKHWKRRFV
160 170 180 190 200
SDVNLARFHR VLKPGGKFCF ASDIDTYVNW TLQHCARHGG FEWTATSADD
210 220 230
WRTPYANWPG TRYENKAKRE GRSSAYLTFI RR
Length:232
Mass (Da):26,864
Last modified:June 1, 2002 - v1
Checksum:i7F8A29F4E1F00423
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86180.1.
PIRiAC2621.
C97403.
RefSeqiNP_353395.1. NC_003062.2.
WP_006310208.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK86180; AAK86180; Atu0363.
GeneIDi1132401.
KEGGiatu:Atu0363.
PATRICi20810409. VBIAgrTum91616_0354.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86180.1.
PIRiAC2621.
C97403.
RefSeqiNP_353395.1. NC_003062.2.
WP_006310208.1. NC_003062.2.

3D structure databases

ProteinModelPortaliQ8UID4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu0363.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK86180; AAK86180; Atu0363.
GeneIDi1132401.
KEGGiatu:Atu0363.
PATRICi20810409. VBIAgrTum91616_0354.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiIGCEPFV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciAGRO:ATU0363-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiTRMB_AGRFC
AccessioniPrimary (citable) accession number: Q8UID4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 1, 2002
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.