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Q8UID4 (TRMB_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:Atu0363
ORF Names:AGR_C_634
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex ›

Protein attributes

Sequence length232 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA (guanine-N7)-methylation

Inferred from electronic annotation. Source: GOC

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 232232tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171284

Regions

Region211 – 2144Substrate binding Potential

Sites

Binding site631S-adenosyl-L-methionine By similarity
Binding site881S-adenosyl-L-methionine By similarity
Binding site1151S-adenosyl-L-methionine By similarity
Binding site1371S-adenosyl-L-methionine By similarity
Binding site1411Substrate By similarity
Binding site1731Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
Q8UID4 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 7F8A29F4E1F00423

FASTA23226,864
        10         20         30         40         50         60 
MTEERRSRAT EAFFGRRKGK PLRNQQVDTI ENLLPLLKID LESVPPQNLV ALFPADVRSI 

        70         80         90        100        110        120 
RLEIGFGGGE HLAHRAVENP ETGFIGVEPF VNSMAKLLAT VRERELMNIR LYDDDATQLL 

       130        140        150        160        170        180 
DWLPEGSIDH IDLLYPDPWP KKKHWKRRFV SDVNLARFHR VLKPGGKFCF ASDIDTYVNW 

       190        200        210        220        230 
TLQHCARHGG FEWTATSADD WRTPYANWPG TRYENKAKRE GRSSAYLTFI RR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE007869 Genomic DNA. Translation: AAK86180.1.
PIRAC2621.
C97403.
RefSeqNP_353395.1. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UID4.
ModBaseSearch...

Protein-protein interaction databases

STRING176299.Atu0363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1132401.
KEGGatu:Atu0363.
PATRIC20810409. VBIAgrTum91616_0354.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMALYPDPWP.
ProtClustDBPRK00121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMB_AGRT5
AccessionPrimary (citable) accession number: Q8UID4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 1, 2002
Last modified: May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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