Q8UI99 (PYRC_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Atu0399
ORF Names:	AGR_C_703

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00219
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00219
Subunit structure	Homodimer By similarity. HAMAP MF_00219
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Dihydroorotase HAMAP MF_00219		PRO_0000147202
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1; via carbamate group By similarity
	Metal binding	99	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	247	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	99	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UI99 [UniParc]. Last modified July 26, 2002. Version 2. Checksum: CCD97B9569F1AF78	FASTA	345	37,950
	10 20 30 40 50 60 MKSLTLRRPD DWHLHLRDGA MLEGVIGDTS RHFARAIIMP NLVPPVVTTA DASAYRERIL 70 80 90 100 110 120 KAIPEGDRFE PLMTLYLTED TVADDVEEGK KSGLITAVKL YPAGATTNSH GGVRDFNKAM 130 140 150 160 170 180 PVLERMAKIG LPLCVHGEVT TPDVDIFDRE KAFIDTVLEP LRQRLPELKV TMEHITTRDG 190 200 210 220 230 240 VDYIKSSNAN LAGSITTHHL IINRNAILVG GIKPHYYCLP VAKREEHRLA LRAAATSGDA 250 260 270 280 290 300 RFFLGTDSAP HVDPLKECAC GCAGIYTSIN TMSCLAHVFE DENALDKLEA FASLNGPAWY 310 320 330 340 GLAPNDETIT LVKREEAVAF PEKIETGAGP VTVFDPMFPL HWDVN

References

[1]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
[2]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK86214.2.
PIR	AF2625. E97407.
RefSeq	NP_353429.2. NC_003062.2.
3D structure databases
ProteinModelPortal	Q8UI99.
SMR	Q8UI99. Positions 2-345.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UI99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1132437.
GenomeReviews	Gene locus Atu0399 in contig AE007869_GR.
KEGG	atu:Atu0399.
PATRIC	20810483. VBIAgrTum91616_0390.
Phylogenomic databases
eggNOG	COG0418.
HOGENOM	HBG628648.
OMA	CLPVAKR.
PhylomeDB	Q8UI99.
ProtClustDB	PRK05451.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU0399-MONOMER.
Family and domain databases
HAMAP	MF_00219. PyrC_type1. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. PyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_AGRT5

Accession

Primary (citable) accession number: Q8UI99

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	July 26, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program