Reviewed, UniProtKB/Swiss-Prot Q8UI98 (PYRE_AGRT5)

Names and origin

Protein names

Recommended name:
    Orotate phosphoribosyltransferase
      Short name=OPRT
      Short name=OPRTase
    EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	Atu0400
ORF Names:	AGR_C_704

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity.
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_0000110662
Regions
	Region	133 – 141	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	107	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	108	1	5-phosphoribose 1-diphosphate By similarity
	Binding site	111	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	113	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	137	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UI98-1 [UniParc]. Last modified June 6, 2002. Version 1. Checksum: 2899B41B8CD6EE69	FASTA	232	25,513
	10 20 30 40 50 60 MNHFTFTDRT VVAELVAKML WEIKAVHFNS ESPYTFASGM KSPVYIDMRK LISFPRIRSA 70 80 90 100 110 120 AMDFAAAAVM REAGFEKFDC VAGGETAGIP FAAFLAERLG LPMIYCRKKP KGHGRNAQIE 130 140 150 160 170 180 GHMPDGARVL VIEDLTTAGG SMFTFIDAIR AAGGIVDHGI ALFYYGIFEE AEARFANGNV 190 200 210 220 230 KLHYLTTWRD VLAVARAEKL FDEKTLSGVE AFLDNPLPWS AKHGGVSELP QS

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK86215.2.
PIR	AG2625. F97407.
RefSeq	NP_353430.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UI98.
Genome annotation databases
GeneID	1132438.
GenomeReviews	Gene locus Atu0400 in contig AE007869_GR.
KEGG	atc:AGR_C_704. atu:Atu0400.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UI98.
Family and domain databases
HAMAP	MF_01208. [Tree]
InterPro	IPR004467. Or_phspho_trans. IPR002375. Pr/py_Pribosyl_transf_CS. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_AGRT5

Accession

Primary (citable) accession number: Q8UI98

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 6, 2002
Last modified:	September 1, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)