Q8UI70 (OTC_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	Atu0429
ORF Names:	AGR_C_755

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000112872
Regions
	Region	54 – 58	5	Carbamoyl phosphate binding By similarity
	Region	261 – 264	4	Ornithine binding By similarity
Sites
	Binding site	105	1	Carbamoyl phosphate By similarity
	Binding site	132	1	Carbamoyl phosphate By similarity
	Site	29	1	Important for structural integrity By similarity
	Site	145	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UI70 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 1CDD72DC07740982	FASTA	305	33,517
	10 20 30 40 50 60 MASPKHFLDL SAVGPQDLRT ILDDARARKV ATKAGTAEKP LAGKMLAMIF EKPSTRTRVS 70 80 90 100 110 120 FDVGMRQLGG ETLFLSGTEM QLGRAETIGD TAKVLSRYVD AIMIRTTDHS RLLELAEHAT 130 140 150 160 170 180 VPVINGLTDD THPCQIMADI MTFEEHRGPV KGKTIAWTGD GNNVLHSFVE GSARFGYRMT 190 200 210 220 230 240 MAVPMGSEPH DKFMNWARNN GGEIALYHDA DKAVAGADCV VTDTWVSMNQ EHKARGHNIF 250 260 270 280 290 300 QPYQVNEALM AKAQKDALFM HCLPAHRGEE VTDAVIDGPQ SVVFDEAENR LHAQKSVIAW CMGVI

References

[1]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
[2]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK86242.1.
PIR	A97411. AC2629.
RefSeq	NP_353457.1. NC_003062.2.
3D structure databases
ProteinModelPortal	Q8UI70.
SMR	Q8UI70. Positions 3-305.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UI70.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1132467.
GenomeReviews	Gene locus Atu0429 in contig AE007869_GR.
KEGG	atu:Atu0429.
PATRIC	20810543. VBIAgrTum91616_0419.
Phylogenomic databases
eggNOG	COG0078.
HOGENOM	HBG579429.
OMA	KHLLSIK.
PhylomeDB	Q8UI70.
ProtClustDB	PRK00779.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU0429-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_AGRT5

Accession

Primary (citable) accession number: Q8UI70

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program