Reviewed, UniProtKB/Swiss-Prot Q8UHP1 (SYGA_AGRT5)

Names and origin

Protein names

Recommended name:
    Glycyl-tRNA synthetase alpha subunit
    EC=6.1.1.14
Alternative name(s):
    Glycine--tRNA ligase alpha subunit
      Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	Atu0639
ORF Names:	AGR_C_1133

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00254
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Glycyl-tRNA synthetase alpha subunit HAMAP MF_00254		PRO_0000072823

Sequences

	Sequence		Length	Mass (Da)
	Q8UHP1-1 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 532B3D9E5D8CE913	FASTA	310	35,055
	10 20 30 40 50 60 MNPKRSFQAL ILTLHNYWAD KGCAVLQPYD MEVGAGTFHP ATTLRALGPK PWKAAYVQPS 70 80 90 100 110 120 RRPSDGRYGE NPNRLQHYYQ YQVILKPNPS NLQELYLGSL KAIGLDPLLH DVRFVEDDWE 130 140 150 160 170 180 SPTLGAWGLG WECWCDGMEV SQFTYFQQVC GIECSPVAGE LTYGLERLAM YVQGVDNVYD 190 200 210 220 230 240 LNFNGREGEE KISYGDVFLQ AEQEYSRHNF EFADTSMLHR HFIDAEKECL ALLAAGAPGD 250 260 270 280 290 300 NDNQRLHKCV FPAYDQCIKA SHIFNLLNAR GVISVTERER YIARVRDLAK ACGEAFLLTD 310 AGGLNWNRAA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK86446.2.
PIR	AI2654. E97436.
RefSeq	NP_353661.2.
3D structure databases
HSSP	HSSP built from PDB template 1J5W based on UniProtKB Q9WY59.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UHP1.
Genome annotation databases
GeneID	1132677.
GenomeReviews	Gene locus Atu0639 in contig AE007869_GR.
KEGG	atc:AGR_C_1133. atu:Atu0639.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UHP1.
Family and domain databases
HAMAP	MF_00254. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
ProDom	PD006985. tRNA_synt_2e. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00388. glyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_AGRT5

Accession

Primary (citable) accession number: Q8UHP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	September 1, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)