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Q8UHN3 (PUR2_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:Atu0647
ORF Names:AGR_C_1152
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151432

Regions

Domain107 – 312206ATP-grasp
Nucleotide binding133 – 19361ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8UHN3 [UniParc].

Last modified November 15, 2002. Version 2.
Checksum: 33A78649486772B8

FASTA42344,339
        10         20         30         40         50         60 
MKVLLIGSGG REHALAWKIA QSPLIDALYA APGNPGIADH ATLVSLDVED HAAVIAFAKE 

        70         80         90        100        110        120 
KAIDFVVIGP EAPLVAGLAD DLRAAGIATF GPSKAAAQLE GSKGFTKDLC ARYDIPTGAY 

       130        140        150        160        170        180 
ERFKAAEPAK DYVRAQGAPI VIKADGLAAG KGVTVAMTEA EALAAIDDCF DGAFGTAGTE 

       190        200        210        220        230        240 
VVVEAFLDGE EASFFCLSDG KTALALATAQ DHKRVGDGDT GPNTGGMGAY SPAPVMTPAM 

       250        260        270        280        290        300 
VERTMKEIIE PTISGMAKDG NPFSGVFFAG LMITAKGPEL IEYNVRFGDP ECQVLMMRLK 

       310        320        330        340        350        360 
SDLLPILYAT ATGTLDKVQA EWRDDAALTV VLASKGYPGA YDKNTPIAHI PEASEEAKVF 

       370        380        390        400        410        420 
HAGTALKDGK LVATGGRVLN VTAFGRNVTE AQARAYALAD KVEWENGFCR RDIGWQAIAR 


EKA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007869 Genomic DNA. Translation: AAK86455.2.
PIRAI2655.
F97437.
RefSeqNP_353670.2. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UHN3.
SMRQ8UHN3. Positions 1-420.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu0647.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK86455; AAK86455; Atu0647.
GeneID1132685.
KEGGatu:Atu0647.
PATRIC20810993. VBIAgrTum91616_0640.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMAIMMRLKT.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-900-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_AGRT5
AccessionPrimary (citable) accession number: Q8UHN3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 15, 2002
Last modified: July 9, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways