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Q8UHJ6

- SYI_AGRT5

UniProt

Q8UHJ6 - SYI_AGRT5

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei600 – 6001Aminoacyl-adenylateUniRule annotation
Binding sitei644 – 6441ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAGRO:ATU0686-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Atu0686
ORF Names:AGR_C_1230
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Isoleucine--tRNA ligasePRO_0000098339Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu0686.

Structurei

3D structure databases

ProteinModelPortaliQ8UHJ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionAdd
BLAST
Motifi641 – 6455"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8UHJ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDTAEKLDY STTLYLPQTD FPMRAGLPQK EPETVKRWQD MGLYKKLRAS
60 70 80 90 100
AAGREKFVLH DGPPYANGNI HIGHALNKIL KDVITRSFQM RGFDANYVPG
110 120 130 140 150
WDCHGLPIEW KIEEAYRARG KNKDEVPVNE FRKECRDFAA GWIKVQSEEF
160 170 180 190 200
KRLGIEGDFE NPYTTMNFHA EARIAGELLK IARSGQLYRG SKPIMWSVVE
210 220 230 240 250
RTALAEAEVE YHDVESDMIW VKFPVKDAAP ALSGVSVVIW TTTPWTIPGN
260 270 280 290 300
RAIAFSSRVE YGLFEVESAQ NDFGPQTGEK LIFARKLADE AAAKAKLTFK
310 320 330 340 350
FVRDVKSEEL AAITCAHPLA SLGYDFPVPL LDGDHVTDDA GTGFVHTAPS
360 370 380 390 400
HGREDFDAWT NSVRTLEARG IDTKIPFPVD DAGFYTEDAP GFGPSAEGGA
410 420 430 440 450
ARVMDDNGKK GDANERVIKA LIAANNLFAR GRLKHSYPHS WRSKKPVIFR
460 470 480 490 500
NTPQWFVYMD KELGDGTTLR SRSLDAIDQT RFVPASGQNR LRAMIEGRPD
510 520 530 540 550
WVLSRQRSWG VPIAVFADEQ GEVLVDEAVN ARILEAFEAE GADAWFAEGA
560 570 580 590 600
KERFLGNDHD HAKWQQVMDI LDVWFDSGCT HTFTLEDRPD MKWPADVYLE
610 620 630 640 650
GSDQHRGWFH SSLLESCATR GRAPYNAVVT HGFTMDEQGR KQSKSLGNVV
660 670 680 690 700
APQDVMKESG ADILRLWVMT TDYWEDQRLG KAIIQTNIDA YRKLRNTVRW
710 720 730 740 750
MLGTLAHDNG EEIAYADLPE LEKLVLHRLS ELDKVVRDGY DAFDFKKITR
760 770 780 790 800
ALIDFANVEL SAFYFDIRKD TLYCDAPSSL KRRASLSVIA KLFDCLVTWL
810 820 830 840 850
APMLPFTTEE AWLSRYPDAE SVHLAQFPEI PAEWKNDALE AKWEKIRKVR
860 870 880 890 900
TVVTGALEVE RREKRIGSSL EAAPVVHIAD SDLLAALSGQ DFAEICITSA
910 920 930 940 950
ITVVGDEGPA DGFRLQEVAK VVVEQKLAEG VKCARSWRIT TDVGSDPQYP
960
DVSARDAAAL RELAALK
Length:967
Mass (Da):108,483
Last modified:June 1, 2002 - v1
Checksum:i709A52EC5623ED0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86495.1.
PIRiAH2660.
F97442.
RefSeqiNP_353710.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK86495; AAK86495; Atu0686.
GeneIDi1138191.
KEGGiatu:Atu0686.
PATRICi20811077. VBIAgrTum91616_0682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86495.1 .
PIRi AH2660.
F97442.
RefSeqi NP_353710.1. NC_003062.2.

3D structure databases

ProteinModelPortali Q8UHJ6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176299.Atu0686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK86495 ; AAK86495 ; Atu0686 .
GeneIDi 1138191.
KEGGi atu:Atu0686.
PATRICi 20811077. VBIAgrTum91616_0682.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AGRO:ATU0686-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiSYI_AGRT5
AccessioniPrimary (citable) accession number: Q8UHJ6
Secondary accession number(s): Q7D0X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3