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Q8UHJ6

- SYI_AGRT5

UniProt

Q8UHJ6 - SYI_AGRT5

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Atu0686, AGR_C_1230
Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei600 – 6001Aminoacyl-adenylate By similarity
Binding sitei644 – 6441ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAGRO:ATU0686-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Atu0686
ORF Names:AGR_C_1230
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 967967Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098339Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu0686.

Structurei

3D structure databases

ProteinModelPortaliQ8UHJ6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi64 – 7411"HIGH" regionUniRule annotation
Add
BLAST
Motifi641 – 6455"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8UHJ6-1 [UniParc]FASTAAdd to Basket

« Hide

MSDTAEKLDY STTLYLPQTD FPMRAGLPQK EPETVKRWQD MGLYKKLRAS    50
AAGREKFVLH DGPPYANGNI HIGHALNKIL KDVITRSFQM RGFDANYVPG 100
WDCHGLPIEW KIEEAYRARG KNKDEVPVNE FRKECRDFAA GWIKVQSEEF 150
KRLGIEGDFE NPYTTMNFHA EARIAGELLK IARSGQLYRG SKPIMWSVVE 200
RTALAEAEVE YHDVESDMIW VKFPVKDAAP ALSGVSVVIW TTTPWTIPGN 250
RAIAFSSRVE YGLFEVESAQ NDFGPQTGEK LIFARKLADE AAAKAKLTFK 300
FVRDVKSEEL AAITCAHPLA SLGYDFPVPL LDGDHVTDDA GTGFVHTAPS 350
HGREDFDAWT NSVRTLEARG IDTKIPFPVD DAGFYTEDAP GFGPSAEGGA 400
ARVMDDNGKK GDANERVIKA LIAANNLFAR GRLKHSYPHS WRSKKPVIFR 450
NTPQWFVYMD KELGDGTTLR SRSLDAIDQT RFVPASGQNR LRAMIEGRPD 500
WVLSRQRSWG VPIAVFADEQ GEVLVDEAVN ARILEAFEAE GADAWFAEGA 550
KERFLGNDHD HAKWQQVMDI LDVWFDSGCT HTFTLEDRPD MKWPADVYLE 600
GSDQHRGWFH SSLLESCATR GRAPYNAVVT HGFTMDEQGR KQSKSLGNVV 650
APQDVMKESG ADILRLWVMT TDYWEDQRLG KAIIQTNIDA YRKLRNTVRW 700
MLGTLAHDNG EEIAYADLPE LEKLVLHRLS ELDKVVRDGY DAFDFKKITR 750
ALIDFANVEL SAFYFDIRKD TLYCDAPSSL KRRASLSVIA KLFDCLVTWL 800
APMLPFTTEE AWLSRYPDAE SVHLAQFPEI PAEWKNDALE AKWEKIRKVR 850
TVVTGALEVE RREKRIGSSL EAAPVVHIAD SDLLAALSGQ DFAEICITSA 900
ITVVGDEGPA DGFRLQEVAK VVVEQKLAEG VKCARSWRIT TDVGSDPQYP 950
DVSARDAAAL RELAALK 967
Length:967
Mass (Da):108,483
Last modified:June 1, 2002 - v1
Checksum:i709A52EC5623ED0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK86495.1.
PIRiAH2660.
F97442.
RefSeqiNP_353710.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK86495; AAK86495; Atu0686.
GeneIDi1138191.
KEGGiatu:Atu0686.
PATRICi20811077. VBIAgrTum91616_0682.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK86495.1 .
PIRi AH2660.
F97442.
RefSeqi NP_353710.1. NC_003062.2.

3D structure databases

ProteinModelPortali Q8UHJ6.
ModBasei Search...

Protein-protein interaction databases

STRINGi 176299.Atu0686.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK86495 ; AAK86495 ; Atu0686 .
GeneIDi 1138191.
KEGGi atu:Atu0686.
PATRICi 20811077. VBIAgrTum91616_0682.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci AGRO:ATU0686-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiSYI_AGRT5
AccessioniPrimary (citable) accession number: Q8UHJ6
Secondary accession number(s): Q7D0X7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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