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Q8UHJ6

- SYI_AGRT5

UniProt

Q8UHJ6 - SYI_AGRT5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei600 – 6001Aminoacyl-adenylateUniRule annotation
    Binding sitei644 – 6441ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciAGRO:ATU0686-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Atu0686
    ORF Names:AGR_C_1230
    OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
    Taxonomic identifieri176299 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    ProteomesiUP000000813: Chromosome circular

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 967967Isoleucine--tRNA ligasePRO_0000098339Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi176299.Atu0686.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8UHJ6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi64 – 7411"HIGH" regionAdd
    BLAST
    Motifi641 – 6455"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8UHJ6-1 [UniParc]FASTAAdd to Basket

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    MSDTAEKLDY STTLYLPQTD FPMRAGLPQK EPETVKRWQD MGLYKKLRAS    50
    AAGREKFVLH DGPPYANGNI HIGHALNKIL KDVITRSFQM RGFDANYVPG 100
    WDCHGLPIEW KIEEAYRARG KNKDEVPVNE FRKECRDFAA GWIKVQSEEF 150
    KRLGIEGDFE NPYTTMNFHA EARIAGELLK IARSGQLYRG SKPIMWSVVE 200
    RTALAEAEVE YHDVESDMIW VKFPVKDAAP ALSGVSVVIW TTTPWTIPGN 250
    RAIAFSSRVE YGLFEVESAQ NDFGPQTGEK LIFARKLADE AAAKAKLTFK 300
    FVRDVKSEEL AAITCAHPLA SLGYDFPVPL LDGDHVTDDA GTGFVHTAPS 350
    HGREDFDAWT NSVRTLEARG IDTKIPFPVD DAGFYTEDAP GFGPSAEGGA 400
    ARVMDDNGKK GDANERVIKA LIAANNLFAR GRLKHSYPHS WRSKKPVIFR 450
    NTPQWFVYMD KELGDGTTLR SRSLDAIDQT RFVPASGQNR LRAMIEGRPD 500
    WVLSRQRSWG VPIAVFADEQ GEVLVDEAVN ARILEAFEAE GADAWFAEGA 550
    KERFLGNDHD HAKWQQVMDI LDVWFDSGCT HTFTLEDRPD MKWPADVYLE 600
    GSDQHRGWFH SSLLESCATR GRAPYNAVVT HGFTMDEQGR KQSKSLGNVV 650
    APQDVMKESG ADILRLWVMT TDYWEDQRLG KAIIQTNIDA YRKLRNTVRW 700
    MLGTLAHDNG EEIAYADLPE LEKLVLHRLS ELDKVVRDGY DAFDFKKITR 750
    ALIDFANVEL SAFYFDIRKD TLYCDAPSSL KRRASLSVIA KLFDCLVTWL 800
    APMLPFTTEE AWLSRYPDAE SVHLAQFPEI PAEWKNDALE AKWEKIRKVR 850
    TVVTGALEVE RREKRIGSSL EAAPVVHIAD SDLLAALSGQ DFAEICITSA 900
    ITVVGDEGPA DGFRLQEVAK VVVEQKLAEG VKCARSWRIT TDVGSDPQYP 950
    DVSARDAAAL RELAALK 967
    Length:967
    Mass (Da):108,483
    Last modified:June 1, 2002 - v1
    Checksum:i709A52EC5623ED0F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007869 Genomic DNA. Translation: AAK86495.1.
    PIRiAH2660.
    F97442.
    RefSeqiNP_353710.1. NC_003062.2.

    Genome annotation databases

    EnsemblBacteriaiAAK86495; AAK86495; Atu0686.
    GeneIDi1138191.
    KEGGiatu:Atu0686.
    PATRICi20811077. VBIAgrTum91616_0682.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007869 Genomic DNA. Translation: AAK86495.1 .
    PIRi AH2660.
    F97442.
    RefSeqi NP_353710.1. NC_003062.2.

    3D structure databases

    ProteinModelPortali Q8UHJ6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176299.Atu0686.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK86495 ; AAK86495 ; Atu0686 .
    GeneIDi 1138191.
    KEGGi atu:Atu0686.
    PATRICi 20811077. VBIAgrTum91616_0682.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci AGRO:ATU0686-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.

    Entry informationi

    Entry nameiSYI_AGRT5
    AccessioniPrimary (citable) accession number: Q8UHJ6
    Secondary accession number(s): Q7D0X7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3