Reviewed, UniProtKB/Swiss-Prot Q8UHD7 (DXS_AGRT5)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Atu0745
ORF Names:	AGR_C_1351

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189080

Sequences

	Sequence		Length	Mass (Da)
	Q8UHD7-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 9FFFA4DE4850E12D	FASTA	639	68,116
	10 20 30 40 50 60 MTGMPQTPLL DRVNFPSDLK EIDDRDLPEL ARELRDEMID AVSKTGGHLG AGLGVVELTI 70 80 90 100 110 120 AIHKVFNTPE DRLIFDVGHQ CYPHKILTGR RDRIRTLRQE DGLSGFTRRA ESEYDDFGAG 130 140 150 160 170 180 HSSTSISAGL GMAVAAGLDG SDRKVIAVIG DGSMSAGMAF EALNNAGALD ARLIVILNDN 190 200 210 220 230 240 DMSIAPPTGA MSAYLARLAS GRTYMGFRDF GKKLTAYLGK TIDRAITRAV THARGYVTGG 250 260 270 280 290 300 TLFEELGFYH IGPIDGHSFD HLLPVLRNVR DNQKGPVLIH VVTQKGKGYA PAEAAADKYH 310 320 330 340 350 360 GVNKFDVITG AQAKAKPNAP SYTSVFAEAL IQEATLDEKI IGVTAAMPNG TGLDKMAELF 370 380 390 400 410 420 PSRTFDVGIA EQHAVTFAAG LAADGYKPFC ALYSTFLQRG YDQLVHDVAI QSLPVRFPID 430 440 450 460 470 480 RAGFVGADGP THAGSFDTTF LATLPGMVVM AAADEAELKH MVRTAAAYDE GPISFRYPRG 490 500 510 520 530 540 EGVGVEMPAR GEILQIGKGR IIKEGTKVAL LSFGTRLAEC LAAAEDLDAA GLSTTVADAR 550 560 570 580 590 600 FAKPLDLDLI RQLAAHHEVL VTIEEGSVGG FGAHVLHFMA SAGLLDHGPK VRTLTLPDQW 610 620 630 VEQAKPETMY ANAGLDRAGI VSTVFNALGQ RQAGVGFAG

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK86554.1.
PIR	A97450. AC2668.
RefSeq	NP_353769.1.
3D structure databases
SMR	Q8UHD7. Positions 3-628.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UHD7.
Genome annotation databases
GeneID	1132783.
GenomeReviews	Gene locus Atu0745 in contig AE007869_GR.
KEGG	atc:AGR_C_1351. atu:Atu0745.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	QRFPDRY.
PhylomeDB	Q8UHD7.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU0745-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_AGRT5

Accession

Primary (citable) accession number: Q8UHD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)