Pyridoxine/pyridoxamine 5'-phosphate oxidase - Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Contribute

Send feedback

Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q8UHC2 (PDXH_AGRT5)

Last modified November 3, 2009. Version 49. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Pyridoxine/pyridoxamine 5'-phosphate oxidase
    EC=1.4.3.5
Alternative name(s):
    PNP/PMP oxidase
      Short name=PNPOx
    Pyridoxal 5'-phosphate synthase

Gene names

Name:	pdxH
Ordered Locus Names:	Atu0760
ORF Names:	AGR_C_1381

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Hide | Top

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

Hide | Top

General annotation (Comments)

Function	Catalyzes the oxidation of either pyridoxine 5'-phosphate (PNP) or pyridoxamine 5'-phosphate (PMP) into pyridoxal 5'-phosphate (PLP) By similarity.
Catalytic activity	Pyridoxamine 5'-phosphate + H₂O + O₂ = pyridoxal 5'-phosphate + NH₃ + H₂O₂. HAMAP MF_01629 Pyridoxine 5'-phosphate + O₂ = pyridoxal 5'-phosphate + H₂O₂. HAMAP MF_01629
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxamine 5'-phosphate: step 1/1. HAMAP MF_01629 Cofactor biosynthesis; B6 vitamer interconversion; pyridoxal 5'-phosphate from pyridoxine 5'-phosphate: step 1/1.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the pyridoxamine 5'-phosphate oxidase family.

Hide | Top

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: HAMAP pyridoxamine-phosphate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 206

206

Pyridoxine/pyridoxamine 5'-phosphate oxidase HAMAP MF_01629

PRO_0000167678

Regions

Nucleotide binding

68 – 69

FMN By similarity

Nucleotide binding

132 – 133

FMN By similarity

Region

183 – 185

Substrate binding By similarity

Sites

Binding site

FMN By similarity

Binding site

FMN; via amide nitrogen By similarity

Binding site

Substrate By similarity

Binding site

FMN By similarity

Binding site

115

Substrate By similarity

Binding site

119

Substrate By similarity

Binding site

123

Substrate By similarity

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q8UHC2-1 [UniParc].

Last modified June 1, 2002. Version 1.
Checksum: 3BE488AE5307C0C1
Show »

FASTA

206

23,721

        10         20         30         40         50         60 
MSETGLTSSD FTEENEPFAL FAEWLKDATA SEINDPNAVA LATVDENGLP NVRMVLLKGV 

        70         80         90        100        110        120 
DDRGFVFYTN FESQKGQEIL GQKKAAMCFH WKSLRRQVRL RGEVEIVSDE EADAYYASRP 

       130        140        150        160        170        180 
RGSRIGAWAS KQSRPLEGRF ALEKAVAEYT ARYAIGEIPR PSHWSGFRIR PVSIEFWHDR 

       190        200 
KFRLHDRIEF RRETPDGAWS KVRMYP

« Hide

Hide | Top

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58."
Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J.

Nester E.W.
Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58."
Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C.

Slater S.
Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Hide | Top

Cross-references

Sequence databases
	AE007869 Genomic DNA. Translation: AAK86569.1.
PIR	AB2670. H97451.
RefSeq	NP_353784.1.
3D structure databases
HSSP	HSSP built from PDB template 1JNW based on UniProtKB P28225.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UHC2.
Genome annotation databases
GeneID	1132798.
GenomeReviews	Gene locus Atu0760 in contig AE007869_GR.
KEGG	atc:AGR_C_1381. atu:Atu0760.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UHC2.
OMA	EPFALFA.
Family and domain databases
HAMAP	MF_01629. [Tree]
InterPro	IPR000659. Pyridoxamine_oxidase. IPR019740. Pyridoxamine_oxidase_CS. IPR019576. Pyridoxamine_oxidase_dimer_C. IPR011576. PyridoxamineP_oxidase_FMN-bd. IPR012349. Split_barrel_FMN_bd. [Graphical view]
Gene3D	G3DSA:2.30.110.10. PNPOx_FMN_bd. 1 hit.
PANTHER	PTHR10851. Pyridox_oxidase. 1 hit.
Pfam	PF10590. PNPOx_C. 1 hit. PF01243. Pyridox_oxidase. 1 hit. [Graphical view]
ProDom	PD006312. Pyridox_oxidase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00558. pdxH. 1 hit.
PROSITE	PS01064. PYRIDOX_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Hide | Top

Entry information

Entry name

PDXH_AGRT5

Accession

Primary (citable) accession number: Q8UHC2
Secondary accession number(s): Q7D0R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents