Q8UHA8 (KHSE_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	Atu0775
ORF Names:	AGR_C_1416

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00301
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00301
Sequence similarities	Belongs to the pseudomonas-type thrB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 322

322

Homoserine kinase HAMAP MF_00301

PRO_0000172184

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

322

Helix Strand Turn

Details...

Helix

9 – 16

8

Beta strand

24 – 30

7

Beta strand

36 – 45

10

Beta strand

48 – 53

6

Helix

61 – 74

14

Beta strand

92 – 94

3

Beta strand

97 – 103

7

Helix

115 – 131

17

Turn

132 – 134

3

Helix

145 – 155

11

Helix

156 – 161

6

Helix

166 – 180

15

Beta strand

187 – 190

4

Helix

196 – 198

3

Beta strand

199 – 202

4

Beta strand

205 – 209

5

Beta strand

216 – 219

4

Helix

220 – 231

12

Helix

241 – 252

12

Helix

259 – 286

28

Helix

299 – 309

11

Helix

314 – 317

4

Sequences

	Sequence		Length	Mass (Da)
	Q8UHA8 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 6F1E6CDC53744272	FASTA	322	36,243
	10 20 30 40 50 60 MAVYTDITED ELRNFLTQYD VGSLTSYKGI AEGVENSNFL LHTTKDPLIL TLYEKRVEKN 70 80 90 100 110 120 DLPFFLGLMQ HLAAKGLSCP LPLPRKDGEL LGELSGRPAA LISFLEGMWL RKPEAKHCRE 130 140 150 160 170 180 VGKALAAMHL ASEGFEIKRP NALSVDGWKV LWDKSEERAD EVEKGLREEI RPEIDYLAAH 190 200 210 220 230 240 WPKDLPAGVI HADLFQDNVF FLGDELSGLI DFYFACNDLL AYDVSICLNA WCFEKDGAYN 250 260 270 280 290 300 VTKGKALLEG YQSVRPLSEA ELEALPLLSR GSALRFFLTR LYDWLTTPAG ALVVKKDPLE 310 320 YLRKLRFHRT IANVAEYGLA GE

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE007869 Genomic DNA. Translation: AAK86584.1.

PIR

AI2671.
G97453.

RefSeq

NP_353799.1. NC_003062.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PPQ	X-ray	2.00	A	1-322	[»]

ProteinModelPortal

Q8UHA8.

SMR

Q8UHA8. Positions 5-320.

ModBase

Search...

Protein-protein interaction databases

STRING

Q8UHA8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1132813.

GenomeReviews

Gene locus Atu0775 in contig AE007869_GR.

KEGG

atu:Atu0775.

PATRIC

20811263. VBIAgrTum91616_0775.

Phylogenomic databases

HOGENOM

HBG309377.

OMA

ICLNAWC.

ProtClustDB

PRK05231.

Enzyme and pathway databases

BioCyc

ATUM176299-1:ATU0775-MONOMER.

Family and domain databases

HAMAP

MF_00301. Homoser_kinase_2.
[Tree]

InterPro

IPR002575. Aminoglycoside_PTrfase.
IPR005280. Homoserine_kinase_ThrB.
IPR011009. Kinase-like_dom.
[Graphical view]

KO

K02204.

Pfam

PF01636. APH. 1 hit.
[Graphical view]

SUPFAM

SSF56112. Kinase_like. 1 hit.

TIGRFAMs

TIGR00938. ThrB_alt. 1 hit.

ProtoNet

Search...

Entry information

Entry name

KHSE_AGRT5

Accession

Primary (citable) accession number: Q8UHA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program