Q8UH62 (CYSA1_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sulfate/thiosulfate import ATP-binding protein CysA 1
EC=3.6.3.25

Alternative name(s):
Sulfate-transporting ATPase 1

Gene names

Name:	cysA1
Ordered Locus Names:	Atu0823
ORF Names:	AGR_C_1506

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Agrobacterium fabrum

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex CysAWTP involved in sulfate/thiosulfate import. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + sulfate(Out) = ADP + phosphate + sulfate(In).
Subunit structure	The complex is composed of two ATP-binding proteins (CysA), two transmembrane proteins (CysT and CysW) and a solute-binding protein (CysP) Potential.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Sulfate transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ATP catabolic process Inferred from electronic annotation. Source: GOC sulfate transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate transmembrane-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Sulfate/thiosulfate import ATP-binding protein CysA 1		PRO_0000092246
Regions
	Domain	3 – 237	235	ABC transporter
	Nucleotide binding	35 – 42	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8UH62 [UniParc]. Last modified August 22, 2003. Version 2. Checksum: B43889EEA07E3990	FASTA	346	38,511
	10 20 30 40 50 60 MEVKVSGITK QFDRFPALND VSLDIRSGEL IALLGPSGSG KTTLLRLIAG LEQPTQGQIF 70 80 90 100 110 120 FGDEDASHRT VQERNVGFVF QHYALFRHMT VADNIAFGLK VRPSASRPPK AEIRRRVSEL 130 140 150 160 170 180 LDMVHLTGLE KRYPTQLSGG QRQRVALARA VAIEPKVLLL DEPFGALDAK VRKELRRWLR 190 200 210 220 230 240 EFHDRTGHTT VFVTHDQEEA LELADRVVVM SQGKIEQVGT ADDVYDTPNS PFVFSFIGES 250 260 270 280 290 300 SSLPVTIRDG HVLFQGESIG INEGGTGEGE LFFRPEDVVL TDEKDALYGK VTTCRRLAGT 310 320 330 340 RIAEIDIANN GHEPYHLEIE VPLNAAVAVG AELRFRPTRW KVFGKK

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK86630.2.
PIR	AG2677. E97459.
RefSeq	NP_353845.2. NC_003062.2.
3D structure databases
ProteinModelPortal	Q8UH62.
ModBase	Search...
Protein-protein interaction databases
STRING	176299.Atu0823.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1132861.
KEGG	atu:Atu0823.
PATRIC	20811357. VBIAgrTum91616_0820.
Phylogenomic databases
eggNOG	COG1118.
KO	K02045.
OMA	MGIRIHR.
ProtClustDB	CLSK863358.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR026251. ABC_ATP-bd_CysA. IPR014769. ABC_CysA_ATP-bd_C. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR005666. Sulph_transpt1. [Graphical view]
PANTHER	PTHR24220:SF48. PTHR24220:SF48. 1 hit.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00968. 3a0106s01. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51237. CYSA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSA1_AGRT5

Accession

Primary (citable) accession number: Q8UH62

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	August 22, 2003
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program