Reviewed, UniProtKB/Swiss-Prot Q8UH55 (BETA_AGRT5)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Atu0830
ORF Names:	AGR_C_1517

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity. HAMAP MF_00750
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity. HAMAP MF_00750
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	Choline dehydrogenase HAMAP MF_00750		PRO_0000205583
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	465	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UH55-1 [UniParc]. Last modified February 16, 2004. Version 2. Checksum: 6AFD59ED94037C16	FASTA	549	60,627
	10 20 30 40 50 60 MQADYVIVGS GSAGSAIAYR LSEDGRYSVI VIEAGGSDFG PFIQMPAALA WPMSMKRYNW 70 80 90 100 110 120 GYLSEPEPNL DNRRITAPRG KVIGGSSSIN GLVYVRGHAE DFNRWEELGA QGWAYADVLP 130 140 150 160 170 180 YFKRMEHSHG GEEGWRGTDG PLHVQRGPVS NPLFHAFIQA GAQAGFELTD DYNGSKQEGF 190 200 210 220 230 240 GLMEQTIHNG RRWSAANAYL KPALKRGNVT LVNGFARKVI IENGRAVGVE IERRGRVETV 250 260 270 280 290 300 KANREVIVSA SSFNSPKLLM LSGIGPAAHL KDMGIEVKAD RPGVGANLQD HMEFYFQQVS 310 320 330 340 350 360 TKPVSLYSWL PWFWQGVAGA QWLLSKGGLG ASNQFEACAF LRSAPGLKQP DIQYHFLPVA 370 380 390 400 410 420 ISYDGKAAAK SHGFQAHVGY NLSKSRGNVT LRSADPHDDP VIRFNYMSHP EDWEKFRHCV 430 440 450 460 470 480 RLTREIFGQK AFDDFRGPEI QPGENIETDE QIDAFLREHL ESAYHPCGTC RMGDRNDPMA 490 500 510 520 530 540 VVDPECRVIG VEGLRVADSS IFPHVTYGNL NGPSIMTGEK AADHILGKTP LPRSNQEPWV NPRAAVSDR

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK86636.2.
PIR	AF2678. C97460.
RefSeq	NP_353851.2.
3D structure databases
SMR	Q8UH55. Positions 2-525.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UH55.
Genome annotation databases
GeneID	1132868.
GenomeReviews	Gene locus Atu0830 in contig AE007869_GR.
KEGG	atc:AGR_C_1517. atu:Atu0830.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2303.
HOGENOM	HBG734713.
OMA	HMTVKNG.
PhylomeDB	Q8UH55.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU0830-MONOMER.
Family and domain databases
HAMAP	MF_00750. Choline_dehydrogen. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_AGRT5

Accession

Primary (citable) accession number: Q8UH55

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	February 16, 2004
Last modified:	February 9, 2010
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)