Reviewed, UniProtKB/Swiss-Prot Q8UG98 (PUR5_AGRT5)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
Alternative name(s):
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase

Gene names

Name:	purM
Ordered Locus Names:	Atu1141
ORF Names:	AGR_C_2111

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 357	357	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741		PRO_0000148193

Sequences

	Sequence		Length	Mass (Da)
	Q8UG98-1 [UniParc]. Last modified November 28, 2003. Version 2. Checksum: C57C009287B9B9CF	FASTA	357	36,799
	10 20 30 40 50 60 MSQSGKNGLT YSDAGVDIDA GNLMVEKIKP AVRSTRRPGA DGEIGGFGGL FDLKAAGFTD 70 80 90 100 110 120 PVLVAANDGV GTKLKIAIDA DYHDTVGIDL VAMCVNDLVV QGAEPLFFLD YFATGKLDPD 130 140 150 160 170 180 QGAAIVSGIA AGCRESGCAL IGGETAEMPG MYSDGDYDLA GFAVGAAERG QLLPAGDIAE 190 200 210 220 230 240 GDVILGLSSS GVHSNGFSLV RKIVSLSGLE WSAPAPFADG KKLGEALLTP TRIYVKPLLK 250 260 270 280 290 300 AIRETGALKA LAHITGGGFP ENIPRVLPKH LAAEIDLAAI KVPAVFSWLA KTGGVEAHEM 310 320 330 340 350 LRTFNCGVGM IVVVSAENAT KVTEALTAEG ETVFPLGRMV AREDGAHGTI YKGTLGL

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK86944.2.
PIR	AD2717. G97498.
RefSeq	NP_354159.2.
3D structure databases
HSSP	HSSP built from PDB template 1CLI based on UniProtKB P08178.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UG98.
Genome annotation databases
GeneID	1133179.
GenomeReviews	Gene locus Atu1141 in contig AE007869_GR.
KEGG	atc:AGR_C_2111. atu:Atu1141.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UG98.
Family and domain databases
HAMAP	MF_00741. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_AGRT5

Accession

Primary (citable) accession number: Q8UG98

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 28, 2003
Last modified:	September 1, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)