Reviewed, UniProtKB/Swiss-Prot Q8UG67 (HPPA_AGRT5)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Synonyms:	vppA
Ordered Locus Names:	Atu1174
ORF Names:	AGR_C_2169

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	714 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient. HAMAP MF_01130
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium. HAMAP MF_01130
Subunit structure	Homodimer Potential.
Subcellular location	Acidocalcisome membrane; Multi-pass membrane protein. Ref.5
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Membrane
Domain	Signal Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 714	694	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000013525
Regions
	Transmembrane	52 – 74	23	Potential
	Transmembrane	79 – 101	23	Potential
	Transmembrane	126 – 148	23	Potential
	Transmembrane	161 – 183	23	Potential
	Transmembrane	237 – 254	18	Potential
	Transmembrane	261 – 283	23	Potential
	Transmembrane	298 – 320	23	Potential
	Transmembrane	332 – 354	23	Potential
	Transmembrane	380 – 402	23	Potential
	Transmembrane	409 – 431	23	Potential
	Transmembrane	475 – 497	23	Potential
	Transmembrane	518 – 540	23	Potential
	Transmembrane	590 – 612	23	Potential
	Transmembrane	619 – 641	23	Potential
	Transmembrane	690 – 712	23	Potential
Sites
	Site	469	1	Determinant of potassium independence By similarity
Experimental info
	Sequence conflict	385	1	A → T in AAL14977. Ref.3
	Sequence conflict	409	1	G → A in AAL14977. Ref.3
	Sequence conflict	450 – 452	3	LDP → VDR Ref.3
	Sequence conflict	639 – 640	2	SM → PC in CAC80978. Ref.4
	Sequence conflict	697 – 700	4	AIKI → GHQD Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q8UG67-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 41C8F6721FBE19CD	FASTA	714	72,792
	10 20 30 40 50 60 MRMTVIPIVI LCGVLSVVYA VWTTKSVLDA DQGNERMREI AGYIREGAQA YLTRQYLTIA 70 80 90 100 110 120 IVGLIVAVLA WYLLSAIAAI GFVIGAVLSG VAGFVGMHVS VRANLRTAQA ASHSLGAGLD 130 140 150 160 170 180 IAFKSGAITG MLVAGLALLG VSIYYFVLTS VLGHPPGSRA VIDALVSLGF GASLISIFAR 190 200 210 220 230 240 LGGGIFTKGA DVGGDLVGKV EAGIPEDDPR NPATIADNVG DNVGDCAGMA ADLFETYAVS 250 260 270 280 290 300 VVATMVLAAI FFAGTPILES AMVYPLAICG ACILTSIAGT FFVKLGTNNS IMGALYKGLI 310 320 330 340 350 360 ATGVFSVAGL AVATYATVGW GTIGTVAGME ITGTNLFFCG LVGLVVTALI VVITEYYTGT 370 380 390 400 410 420 NKRPVNSIAQ ASVTGHGTNV IQGLAVSLES TALPAIVIVG GIIGTYQLGG LFGTGIAVTA 430 440 450 460 470 480 MLGLAGMIVA LDAFGPVTDN AGGIAEMAGL DPDVRKATDA LDAVGNTTKA VTKGYAIGSA 490 500 510 520 530 540 GLGALVLFAA YANDLSYFAA NGDTYPYFKD IGEISFSLAN PYVVAGLLFG GLIPYLFGGI 550 560 570 580 590 600 AMTAVGKAAS AIVEEVRRQF REKPGIMAGT EKPDYGRAVD LLTKAAIREM VIPSLLPVLA 610 620 630 640 650 660 PLVVYFGVLL ISGSKASAFA ALGASLLGVI INGLFVAISM TSGGGAWDNA KKSFEDGFID 670 680 690 700 710 KDGVRHVKGS EAHKASVTGD TVGDPYKDTA GPAVNPAIKI TNIVALLLLA VLAH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"High prevalence of the H+-proton-pumping pyrophosphatase gene in alpha proteobacteria and evidence of lateral transfer during its phylogeny." Jumas-Bilak E., Michaux-Charachon S., Teyssier C., Ramuz M. Submitted (SEP-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 248-454 AND 480-700.
[4]	"Presence of proton-translocating pyrophosphatase genes in root nodule-making bacteria (Rhizobia) and pathogenic tumour-making bacteria (Agrobacterium)." Perez-Castineira J.R., Losada M., Serrano A. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 441-644.
[5]	"Identification of organelles in bacteria similar to acidocalcisomes of unicellular eukaryotes." Seufferheld M., Vieira M.C.F., Ruiz F.A., Rodrigues C.O., Moreno S.N.J., Docampo R. J. Biol. Chem. 278:29971-29978(2003) [PubMed: 12783865] [Abstract] Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK86977.2. Different initiation. AF417520 Genomic DNA. Translation: AAL14977.1. AF417521 Genomic DNA. Translation: AAL14978.1. AJ251784 Genomic DNA. Translation: CAC80978.1.
PIR	AD2721. H97502.
RefSeq	NP_354192.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UG67.
Genome annotation databases
GeneID	1133212.
GenomeReviews	Gene locus Atu1174 in contig AE007869_GR.
KEGG	atc:AGR_C_2169. atu:Atu1174.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UG67.
OMA	PYLFGGI.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_AGRT5

Accession

Primary (citable) accession number: Q8UG67
Secondary accession number(s): Q8VPZ1, Q93AR6, Q93AR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)