Reviewed, UniProtKB/Swiss-Prot Q8UFV9 (SYP_AGRT5)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	Atu1288
ORF Names:	AGR_C_2372

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01570
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01570
Subunit structure	Homodimer By similarity. HAMAP MF_01570
Subcellular location	Cytoplasm By similarity HAMAP MF_01570.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Prolyl-tRNA synthetase HAMAP MF_01570		PRO_0000248886

Sequences

	Sequence		Length	Mass (Da)
	Q8UFV9-1 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: 33B8195D3A4560B2	FASTA	440	49,609
	10 20 30 40 50 60 MRLSRYFLPI LKENPKEAEI VSHRLMLRAG MIRQQSAGIY SWLPLGKRVL DKVNKIIREE 70 80 90 100 110 120 QNRAGAIELL MPTLQTAELW QESGRYDDYG KEMLRIKDRQ DRQMLYGPTN EEMITDIFRS 130 140 150 160 170 180 YVKSYKNLPL NLYHIQLKFR DEVRPRFGTM RSREFLMKDA YSFDLTKEDA IHSYNKMFVA 190 200 210 220 230 240 YLRTFERLGL RAIPMRADTG PIGGNHSHEF IILADTGESE VFCHKSFLDR AIPAESTDFD 250 260 270 280 290 300 DVAALQGVFD EWTADYAATS EMHDDAAYDA IPEGERLSAR GIEVGHIFYF GTKYSEPMGA 310 320 330 340 350 360 KVQGKDGKEH PVHMGSYGIG PTRLVPAIIE ASHDENGIIW PASVAPFDVV IINMKAGDAA 370 380 390 400 410 420 CDAACEKLYY QLSNAGKDVL YDDTDDRAGQ KFATADLIGV PVQIIVGPRS VANGEVEVKD 430 440 RKTGERETVT IEAAMNKALG

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK87082.2.
PIR	A97516. AH2734.
RefSeq	NP_354297.2.
3D structure databases
SMR	Q8UFV9. Positions 1-431.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UFV9.
Genome annotation databases
GeneID	1133326.
GenomeReviews	Gene locus Atu1288 in contig AE007869_GR.
KEGG	atc:AGR_C_2372. atu:Atu1288.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG403504.
OMA	DFVLGPT.
PhylomeDB	Q8UFV9.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU1288-MONOMER.
Family and domain databases
HAMAP	MF_01570. Pro_tRNA_synth_type2. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II_cons-dom. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_AGRT5

Accession

Primary (citable) accession number: Q8UFV9
Secondary accession number(s): Q7CZK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	February 9, 2010
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)