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Q8UFV9 (SYP_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Proline--tRNA ligase
EC=6.1.1.15
Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS
Gene names
Name:proS
Ordered Locus Names:Atu1288
ORF Names:AGR_C_2372
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length440 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01570

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01570

Subunit structure

Homodimer By similarity. HAMAP MF_01570

Subcellular location

Cytoplasm By similarity HAMAP MF_01570.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

proline-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 440440Proline--tRNA ligase HAMAP MF_01570
PRO_0000248886

Sequences

Sequence LengthMass (Da)Tools
Q8UFV9 [UniParc].

Last modified July 5, 2004. Version 2.
Checksum: 33B8195D3A4560B2

FASTA44049,609
        10         20         30         40         50         60 
MRLSRYFLPI LKENPKEAEI VSHRLMLRAG MIRQQSAGIY SWLPLGKRVL DKVNKIIREE 

        70         80         90        100        110        120 
QNRAGAIELL MPTLQTAELW QESGRYDDYG KEMLRIKDRQ DRQMLYGPTN EEMITDIFRS 

       130        140        150        160        170        180 
YVKSYKNLPL NLYHIQLKFR DEVRPRFGTM RSREFLMKDA YSFDLTKEDA IHSYNKMFVA 

       190        200        210        220        230        240 
YLRTFERLGL RAIPMRADTG PIGGNHSHEF IILADTGESE VFCHKSFLDR AIPAESTDFD 

       250        260        270        280        290        300 
DVAALQGVFD EWTADYAATS EMHDDAAYDA IPEGERLSAR GIEVGHIFYF GTKYSEPMGA 

       310        320        330        340        350        360 
KVQGKDGKEH PVHMGSYGIG PTRLVPAIIE ASHDENGIIW PASVAPFDVV IINMKAGDAA 

       370        380        390        400        410        420 
CDAACEKLYY QLSNAGKDVL YDDTDDRAGQ KFATADLIGV PVQIIVGPRS VANGEVEVKD 

       430        440 
RKTGERETVT IEAAMNKALG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE007869 Genomic DNA. Translation: AAK87082.2.
PIRA97516.
AH2734.
RefSeqNP_354297.2. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UFV9.
SMRQ8UFV9. Positions 1-431.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8UFV9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1133326.
GenomeReviewsGene locus Atu1288 in contig AE007869_GR.
KEGGatu:Atu1288.
PATRIC20812339. VBIAgrTum91616_1304.

Phylogenomic databases

eggNOGCOG0442.
HOGENOMHBG403504.
OMAIQPAELW.
PhylomeDBQ8UFV9.
ProtClustDBPRK12325.

Enzyme and pathway databases

BioCycATUM176299-1:ATU1288-MONOMER.

Family and domain databases

HAMAPMF_01570. Pro_tRNA_synth_type2.
[Tree]
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-synth_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023716. Prolyl-tRNA_Synthase_IIa_type2.
[Graphical view]
Gene3DG3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KOK01881.
PANTHERPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. Anticodon_bd. 1 hit.
TIGRFAMsTIGR00409. ProS_fam_II. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYP_AGRT5
AccessionPrimary (citable) accession number: Q8UFV9
Secondary accession number(s): Q7CZK5
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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