Reviewed, UniProtKB/Swiss-Prot Q8UFK2 (LEXA_AGRT5)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	Atu1395
ORF Names:	AGR_C_2577

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	LexA repressor HAMAP MF_00015		PRO_0000169998
Regions
	DNA binding	26 – 46	21	H-T-H motif By similarity
Sites
	Active site	160	1	For autocatalytic cleavage activity By similarity
	Active site	198	1	For autocatalytic cleavage activity By similarity
	Site	125 – 126	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UFK2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 2FA935A5EBA20180	FASTA	240	26,033
	10 20 30 40 50 60 MLTRKQQELL LFIHERMKES GVPPSFDEMK DALDLASKSG IHRLITALEE RGFIRRLPNR 70 80 90 100 110 120 ARALEVIKLP EAYTPGARPQ RGFSPSVIEG SLGKPKEPEP APAVKAPAND FAGAATIPVM 130 140 150 160 170 180 GRIAAGVPIS AIQNNTHDLA VPVDMLGSGE HYALEVKGDS MIEAGIFDGD TVIIRNGNTA 190 200 210 220 230 240 NPGDIVVALV DDEEATLKRF RRKGASIALE AANPAYETRI FGPDRVKIQG KLVGLIRRYH

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK87187.1.
PIR	AC2748. B97529.
RefSeq	NP_354402.1.
3D structure databases
SMR	Q8UFK2. Positions 117-240.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UFK2.
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	1133433.
GenomeReviews	Gene locus Atu1395 in contig AE007869_GR.
KEGG	atc:AGR_C_2577. atu:Atu1395.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HBG679610.
OMA	AILTFIR.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU1395-MONOMER.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_AGRT5

Accession

Primary (citable) accession number: Q8UFK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	June 1, 2002
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)