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Q8UFG1

- LIPA_AGRT5

UniProt

Q8UFG1 - LIPA_AGRT5

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Protein
Lipoyl synthase
Gene
lipA, Atu1436, AGR_C_2646
Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi87 – 871Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi91 – 911Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAGRO:ATU1436-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:Atu1436
ORF Names:AGR_C_2646
OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
ProteomesiUP000000813: Chromosome circular

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Lipoyl synthaseUniRule annotation
PRO_0000102281Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176299.Atu1436.

Structurei

3D structure databases

ProteinModelPortaliQ8UFG1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiTIRAVRH.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8UFG1-1 [UniParc]FASTAAdd to Basket

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MVTILDRTSS DEKRIRHPEK AHRPDTEVMR KPEWIRVKAP TSKGYHETRE    50
LVRSHKLVTV CEEAGCPNIG ECWEKKHATF MIMGEICTRA CAFCNVATGK 100
PNALDMDEPE NVAKAVKQMG LSHVVITSVD RDDLADGGAE HFEKVIWAIR 150
AASPTTTIEI LTPDFLKKPG ALERVVAAKP DVFNHNMETV PGNYLTVRPG 200
ARYFHSVRLL QRVKELDPTM FTKSGIMVGL GEERNEVLQL MDDLRSADVD 250
FLTIGQYLQP TRKHHKVEAF VTPEEFKSYE TVAYAKGFLM VSSSPLTRSS 300
HHAGDDFERL RAAREKKLLA AAE 323
Length:323
Mass (Da):36,341
Last modified:June 1, 2002 - v1
Checksum:i74B294773BC784D2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK87227.1.
PIRiAD2753.
B97534.
RefSeqiNP_354442.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK87227; AAK87227; Atu1436.
GeneIDi1133474.
KEGGiatu:Atu1436.
PATRICi20812645. VBIAgrTum91616_1457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE007869 Genomic DNA. Translation: AAK87227.1 .
PIRi AD2753.
B97534.
RefSeqi NP_354442.1. NC_003062.2.

3D structure databases

ProteinModelPortali Q8UFG1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176299.Atu1436.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK87227 ; AAK87227 ; Atu1436 .
GeneIDi 1133474.
KEGGi atu:Atu1436.
PATRICi 20812645. VBIAgrTum91616_1457.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi TIRAVRH.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci AGRO:ATU1436-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C58 / ATCC 33970.

Entry informationi

Entry nameiLIPA_AGRT5
AccessioniPrimary (citable) accession number: Q8UFG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: June 1, 2002
Last modified: May 14, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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