Reviewed, UniProtKB/Swiss-Prot Q8UFA2 (SYM_AGRT5)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Synonyms:	metS
Ordered Locus Names:	Atu1496
ORF Names:	AGR_C_2757

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_01228
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Methionyl-tRNA synthetase HAMAP MF_01228		PRO_0000139204
Regions
	Motif	14 – 24	11	"HIGH" region HAMAP MF_01228
	Motif	302 – 306	5	"KMSKS" region HAMAP MF_01228
Sites
	Binding site	305	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UFA2-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: 4A4FD12C9BF57273	FASTA	516	57,931
	10 20 30 40 50 60 MTDKTPFYIT TAISYPNGKP HIGHAYELIA TDAMARFQRL DGMDVFFLTG TDEHGQKMQQ 70 80 90 100 110 120 TARAEGISPE ELAQRNSDQF REMGKLLNAS NDDFIRTTEE RHHETSRNIW NLMADSGDIY 130 140 150 160 170 180 KDSYAGWYSV RDEAYYAEDE TEVRADGVRY GPQGTPVEWV EEESYFFKLS EYQEKLLKLY 190 200 210 220 230 240 EENPDFIGPA ERRNEIISFV KSGLKDLSIS RTTFDWGIKV PDDPKHVMYV WVDALTNYIT 250 260 270 280 290 300 ATGYIEDRNG PRAKYWPADV HIIGKDIIRF HAVYWPAFLM SAKLPLPKRV YAHGFLLNKG 310 320 330 340 350 360 EKMSKSLGNV VDPANLVAHF GLDPVRYFFM REVSFGQDGS YSEEGIATRI NADLANGIGN 370 380 390 400 410 420 LASRSLSMIV KNCDGQIPTP GAFNDDDKAM LASADGLLAV CREEMGKQLI HRALAAIIAV 430 440 450 460 470 480 VSETDRYFAS QEPWALKKTD PERMGTVLYV TAEVVRQIGI LLQPFMPQSC EKLLDLVAAP 490 500 510 ADGRDFTALG EAGRLAPGTP LEAPKPVFPR YVAPEA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK87287.1.
PIR	AH2760. F97541.
RefSeq	NP_354502.1.
3D structure databases
HSSP	HSSP built from PDB template 1A8H based on UniProtKB P23395.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UFA2.
Genome annotation databases
GeneID	1133534.
GenomeReviews	Gene locus Atu1496 in contig AE007869_GR.
KEGG	atc:AGR_C_2757. atu:Atu1496.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UFA2.
OMA	KMSKYAD.
Family and domain databases
HAMAP	MF_01228. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_AGRT5

Accession

Primary (citable) accession number: Q8UFA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	June 1, 2002
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)