Reviewed, UniProtKB/Swiss-Prot Q8UET6 (TRMFO_AGRT5)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid
Ordered Locus Names:	Atu1669
ORF Names:	AGR_C_3069

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 481	481	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000117225
Regions
	Nucleotide binding	13 – 18	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UET6-1 [UniParc]. Last modified November 28, 2002. Version 2. Checksum: 96A3366044F91EA8	FASTA	481	51,801
	10 20 30 40 50 60 MQDKTHSPIH VVGGGLAGSE AAWQIAESGV PVILHEMRGV RGTDAHKGDT LAELVCSNSF 70 80 90 100 110 120 RSDDATANAV GVIHAEMRLA GSLIIACADK HQVPAGGALA VDRDGFSQAV TDMLENHPLV 130 140 150 160 170 180 TVVREEVTGL PPKEWGSTVI ATGPLTSPDL AAAVQAETGE DALAFFDAIA PILHRDSINM 190 200 210 220 230 240 DICWYQSRYD KVGPGGTGKD YINCPMDEEQ YNVFIDALIA GDTVGFKEWE GTPYFDGCLP 250 260 270 280 290 300 IEIMAERGRQ TLRHGPMKPM GLTNAHNPTV KAYAVVQLRQ DNALGTLYNM VGFQTKLKYS 310 320 330 340 350 360 VQADVFRMIP GLENAEFARL GGLHRNTYID SPILLDRSLR LKSRPDLRFA GQITGCEGYV 370 380 390 400 410 420 ESASVGLLAG RFAAAERKGE APSLPPATTA LGSLLNHITG GHLSSDDEPG KRSFQPMNIN 430 440 450 460 470 480 FGLFPELEPG SIVKPDGVKR FRGKDKTIMK RQLVAARALK DCAAWLGVER VATEQESDAT P

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE007869 Genomic DNA. Translation: AAK87440.2.
PIR	AG2781. G97560.
RefSeq	NP_354655.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1133707.
GenomeReviews	Gene locus Atu1669 in contig AE007869_GR.
KEGG	atc:AGR_C_3069. atu:Atu1669.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UET6.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_AGRT5

Accession

Primary (citable) accession number: Q8UET6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
Last sequence update:	November 28, 2002
Last modified:	June 16, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)