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Q8UET6 (TRMFO_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Synonyms:gid
Ordered Locus Names:Atu1669
ORF Names:AGR_C_3069
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970)
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length481 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037

Cofactor

FAD By similarity. HAMAP MF_01037

Subcellular location

Cytoplasm By similarity HAMAP MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 481481Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP MF_01037
PRO_0000117225

Regions

Nucleotide binding13 – 186FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8UET6 [UniParc].

Last modified November 28, 2002. Version 2.
Checksum: 96A3366044F91EA8

FASTA48151,801
        10         20         30         40         50         60 
MQDKTHSPIH VVGGGLAGSE AAWQIAESGV PVILHEMRGV RGTDAHKGDT LAELVCSNSF 

        70         80         90        100        110        120 
RSDDATANAV GVIHAEMRLA GSLIIACADK HQVPAGGALA VDRDGFSQAV TDMLENHPLV 

       130        140        150        160        170        180 
TVVREEVTGL PPKEWGSTVI ATGPLTSPDL AAAVQAETGE DALAFFDAIA PILHRDSINM 

       190        200        210        220        230        240 
DICWYQSRYD KVGPGGTGKD YINCPMDEEQ YNVFIDALIA GDTVGFKEWE GTPYFDGCLP 

       250        260        270        280        290        300 
IEIMAERGRQ TLRHGPMKPM GLTNAHNPTV KAYAVVQLRQ DNALGTLYNM VGFQTKLKYS 

       310        320        330        340        350        360 
VQADVFRMIP GLENAEFARL GGLHRNTYID SPILLDRSLR LKSRPDLRFA GQITGCEGYV 

       370        380        390        400        410        420 
ESASVGLLAG RFAAAERKGE APSLPPATTA LGSLLNHITG GHLSSDDEPG KRSFQPMNIN 

       430        440        450        460        470        480 
FGLFPELEPG SIVKPDGVKR FRGKDKTIMK RQLVAARALK DCAAWLGVER VATEQESDAT 


P

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE007869 Genomic DNA. Translation: AAK87440.2.
PIRAG2781.
G97560.
RefSeqNP_354655.2. NC_003062.2.

3D structure databases

ProteinModelPortalQ8UET6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8UET6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1133707.
GenomeReviewsGene locus Atu1669 in contig AE007869_GR.
KEGGatu:Atu1669.
PATRIC20813115. VBIAgrTum91616_1686.

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHBG285041.
OMARFAGQIT.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycATUM176299-1:ATU1669-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
[Tree]
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
KOK04094.
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. Gid_trmFO. 1 hit.
PROSITEPS01280. GIDA_1. False negative.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_AGRT5
AccessionPrimary (citable) accession number: Q8UET6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 28, 2002
Last modified: January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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