Q8UEQ7 (MOBA_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Molybdenum cofactor guanylyltransferase
Short name=MoCo guanylyltransferase
EC=2.7.7.77

Alternative name(s):
GTP:molybdopterin guanylyltransferase
Mo-MPT guanylyltransferase
Molybdopterin guanylyltransferase
Molybdopterin-guanine dinucleotide synthase
Short name=MGD synthase

Gene names

Name:	mobA
Ordered Locus Names:	Atu1698
ORF Names:	AGR_C_3120

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Agrobacterium fabrum

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers a GMP moiety from GTP to Mo-molybdopterin (Mo-MPT) cofactor (Moco or molybdenum cofactor) to form Mo-molybdopterin guanine dinucleotide (Mo-MGD) cofactor By similarity. HAMAP-Rule MF_00316
Catalytic activity	GTP + molybdenum cofactor = diphosphate + guanylyl molybdenum cofactor. HAMAP-Rule MF_00316
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00316
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00316
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00316.
Domain	The N-terminal domain determines nucleotide recognition and specific binding, while the C-terminal domain determines the specific binding to the target protein By similarity. HAMAP-Rule MF_00316
Sequence similarities	Belongs to the MobA family.

Ontologies

Keywords
Biological process	Molybdenum cofactor biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP guanylyltransferase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 218	218	Molybdenum cofactor guanylyltransferase HAMAP-Rule MF_00316		PRO_0000134877
Regions
	Nucleotide binding	18 – 20	3	GTP By similarity
Sites
	Metal binding	113	1	Magnesium By similarity
	Binding site	30	1	GTP By similarity
	Binding site	58	1	GTP By similarity
	Binding site	78	1	GTP By similarity
	Binding site	113	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8UEQ7 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: A95970D68BB09A94	FASTA	218	23,402
	10 20 30 40 50 60 MIAPPRKIPG LVPPGLILAG GLSRRMGSNK AMVTLGDAPL LSHVIRRVTP QVSDVTINAA 70 80 90 100 110 120 ITDKGSWAEG FGLPLLPDTL NGHAGPLAGV LAGMRHFRDR ETAGSHFLTA PADSPFFPND 130 140 150 160 170 180 LVVRLCEHLS DDAIVIAASS GQLHPVFALW PVALADDLED WLKNDANRRI RAFLARHVTI 190 200 210 GVAFPPLETA RGSLDPFFNI NTPDELALAR SQLETMET

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK87470.1.
PIR	AD2785. E97564.
RefSeq	NP_354685.1. NC_003062.2.
3D structure databases
ProteinModelPortal	Q8UEQ7.
ModBase	Search...
Protein-protein interaction databases
STRING	176299.Atu1698.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1133736.
KEGG	atu:Atu1698.
PATRIC	20813167. VBIAgrTum91616_1712.
Phylogenomic databases
eggNOG	COG0746.
HOGENOM	HOG000280423.
KO	K03752.
OMA	EMHPLFA.
ProtClustDB	PRK00317.
Family and domain databases
HAMAP	MF_00316. MobA.
InterPro	IPR025877. MobA-like_NTP_Trfase_dom. IPR013482. Molybde_CF_guanTrfase. [Graphical view]
Pfam	PF12804. NTP_transf_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR02665. molyb_mobA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MOBA_AGRT5

Accession

Primary (citable) accession number: Q8UEQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program