Seryl-tRNA synthetase - Agrobacterium tumefaciens (strain C58 / ATCC 33970)

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Reviewed, UniProtKB/Swiss-Prot Q8UEQ2 (SYS_AGRT5)

Last modified September 1, 2009. Version 40. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Seryl-tRNA synthetase
    EC=6.1.1.11
Alternative name(s):
    Seryl-tRNA(Ser/Sec) synthetase
    Serine--tRNA ligase
      Short name=SerRS

Gene names

Name:	serS
Ordered Locus Names:	Atu1703
ORF Names:	AGR_C_3129

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Complete proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

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Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). HAMAP MF_00176 ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec). HAMAP MF_00176
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1. HAMAP MF_00176
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

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Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	selenocysteine biosynthetic process Inferred from electronic annotation. Source: HAMAP seryl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP serine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 427

427

Seryl-tRNA synthetase HAMAP MF_00176

PRO_0000121992

Regions

Nucleotide binding

262 – 264

ATP By similarity

Nucleotide binding

349 – 352

ATP By similarity

Region

231 – 233

Serine binding By similarity

Sites

Binding site

285

Serine By similarity

Binding site

385

Serine By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

Q8UEQ2-1 [UniParc].

Last modified April 23, 2003. Version 2.
Checksum: 95FA03327A365C26
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FASTA

427

47,600

        10         20         30         40         50         60 
MHDIKWIREN PEAFDAALAR RSVEPAAAGL IALDEKRRSV IQSLQDMQSR RNAASKEIGA 

        70         80         90        100        110        120 
AMAQKNMELA EKLKAEVADI KENMPRAEEE DRQVSAELND ALSRLPNMPF DDVPDGKDEH 

       130        140        150        160        170        180 
DNVVARVTGQ KPGWNHAAKE HFEIGEALGY MDFERAAKLS GSRFTVLTSQ LARLERALGQ 

       190        200        210        220        230        240 
FMIDLHTSEH GYTEVSSPLM VRDEAMFGTG QLPKFAEDLF RTTDGRWLIP TAEVTLTNLV 

       250        260        270        280        290        300 
SGEILEQEKL PLRFTALTPS FRSEAGSAGR DTRGMLRQHQ FWKCELVSIT DAESAVAEHE 

       310        320        330        340        350        360 
RMTACAEEVL KRLGLHFRTL TLCTGDMGFS ARKTYDLEVW LPGQNTYREI SSCSVCGDFQ 

       370        380        390        400        410        420 
ARRMNARYRG KDDKATKFVH TLNGSGTAVG RCLIAVLENY LNDDGSVTIP DVLLPYMGGL 


TRIEKAA

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References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58."
Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J.

Nester E.W.
Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58."
Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C.

Slater S.
Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases
	AE007869 Genomic DNA. Translation: AAK87476.2.
PIR	AI2785. C97565.
RefSeq	NP_354691.2.
3D structure databases
HSSP	HSSP built from PDB template 1SER based on UniProtKB P34945.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8UEQ2.
Genome annotation databases
GeneID	1133741.
GenomeReviews	Gene locus Atu1703 in contig AE007869_GR.
KEGG	atc:AGR_C_3129. atu:Atu1703.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8UEQ2.
Family and domain databases
HAMAP	MF_00176. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002317. Ser-tRNA-synth_IIa. IPR018156. Ser-tRNA-synth_IIa_C. IPR015866. Ser-tRNA-synth_IIa_N. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
TIGRFAMs	TIGR00414. serS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

SYS_AGRT5

Accession

Primary (citable) accession number: Q8UEQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	April 23, 2003
Last modified:	September 1, 2009
This is version 40 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents